Th1 and Th2 immune response to P30 and ROP18 peptides in human toxoplasmosis

Torres-Morales, Elizabeth; Taborda, Laura; Cardona, Néstor; de-la-Torre, Alejandra; Sepúlveda-Arias, Juan Carlos; Patarroyo, Manuel A.; Gómez-Marín, Jorge Enrique

doi:10.1007/s00430-014-0339-0

Cited by 44 publications

(23 citation statements)

References 24 publications

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“…Although the eye has immune privilege, the ocular immune response is connected with the peripheral response. We previously demonstrated that patients with ocular toxoplasmosis have different peripheral immune responses compared to those of patients without ocular involvement (9). Both retina and brain are damaged by Toxoplasma infection in humans.…”

Section: Discussionmentioning

confidence: 99%

“…OT is characterized by intraocular inflammation and is the most common clinical manifestation of toxoplasmosis (7). Particularly, OT causes severe pathology in the eye in South America that is more severe than in other parts of the world, and it is characterized by deviated T helper 2 (Th2) immune responses (8,9). Lesions can originate both from congenital infection and from infections acquired after birth (10,11).…”

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confidence: 99%

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Genetic Polymorphisms in Cytokine Genes in Colombian Patients with Ocular Toxoplasmosis

et al. 2018

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Toxoplasmosis is caused by infection with the protozoan parasite , which has the capacity to infect all warm-blooded animals worldwide. Toxoplasmosis is a major cause of visual defects in the Colombian population; however, the association between genetic polymorphisms in cytokine genes and susceptibility to ocular toxoplasmosis has not been studied in this population. This work evaluates the associations between polymorphisms in genes coding for the cytokines tumor necrosis factor alpha (TNF-α) (rs1799964, rs1800629, rs1799724, rs1800630, and rs361525), interleukin 1β (IL-1β) (rs16944, rs1143634, and rs1143627), IL-1α (rs1800587), gamma interferon (IFN-γ) (rs2430561), and IL-10 (rs1800896 and rs1800871) and the presence of ocular toxoplasmosis (OT) in a sample of a Colombian population (61 patients with OT and 116 healthy controls). Genotyping was performed with the "dideoxynucleotide (ddNTP) primer extension" technique. Functional-effect predictions of single nucleotide polymorphisms (SNPs) were done by using FuncPred. A polymorphism in the IL-10 gene promoter (-1082G/A) was significantly more prevalent in OT patients than in controls ( = 1.93e-08; odds ratio [OR] = 5.27e+03; 95% confidence interval [CI] = 3.18 to 8.739; Bonferroni correction [BONF] = 3.48e-07). In contrast, haplotype "AG" of the IL-10 gene promoter polymorphisms (rs1800896 and rs1800871) was present at a lower frequency in OT patients ( = 7e-04; OR = 0.10; 95% CI = 0.03 to 0.35). The +874A/T polymorphism of IFN-γ was associated with OT ( = 3.37e-05; OR = 4.2; 95% CI = 2.478 to 7.12; BONF = 6.07e-04). Haplotype "GAG" of the IL-1β gene promoter polymorphisms (rs1143634, rs1143627, and rs16944) appeared to be significantly associated with OT ( = 0.0494). The IL-10, IFN-γ, and IL-1β polymorphisms influence the development of OT in the Colombian population.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Genetic Polymorphisms in Cytokine Genes in Colombian Patients with Ocular Toxoplasmosis

et al. 2018

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“…Recently, scientists in Colombia have attempted to correlate severity of clinical toxoplasmosis in patients with genetic make of the strain and host responses (cytokine production), and found that the virulent allele of T. gondii ROP18 in ocular toxoplasmosis was correlated with severe ocular inflammatory response [ 98 ]. This study additionally found that the cytokine profile in Colombian patients with ocular toxoplasmosis was deviated to a Th2 profile [ 99 ]; instead, French patients had a Th1 preferential response [ 100 ]. Altogether, these results indicate that some Colombian strains cause more severe ocular toxoplasmosis due to an inhibition of the protective effect of IFN-γ.…”

Section: Reviewmentioning

confidence: 99%

An overview of seventy years of research (1944 – 2014) on toxoplasmosis in Colombia, South America

et al. 2014

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This paper summarizes prevalence of Toxoplasma gondii in humans and animals and associated correlates of infection, clinical spectrum of disease in humans, and genetic diversity of T. gondii isolates from Colombia. Recent studies, especially in the states of Antioquia, Quindío and Cundinamarca, indicate that toxoplasmosis is a major public health problem. Approximately half of the women of child bearing age have T. gondii antibodies, and the clinical disease in congenitally infected children is more severe than in Europe. Limited studies indicate that the strains of T. gondii from Colombia are genetically and phenotypically different than in Europe and North America. However, epidemiological factors, such as the involvement of domestic and/or wild animals in transmission, the distribution of strain diversity by natural geographic regions, and the variation in risk factors between regions that are associated with human infection in Colombia, remain unknown. Areas of research for the future are outlined. This review should be of interest to biologists, veterinarians, physicians, and parasitologists.

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“…In the present study, oral infections of sheep with T. gondii tissue cysts were performed to determine the entry site and subsequent distribution of the parasite following the natural infection route. Furthermore, we monitored the local IFN-gamma response during the acute phase of the infection, because of its important role as a mediator of host resistance against Toxoplasma as demonstrated in mice, sheep and humans [ 17 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Parasite distribution and associated immune response during the acute phase of Toxoplasma gondiiinfection in sheep

Verhelst

Craeye

Entrican³

et al. 2014

BMC Vet Res

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BackgroundIn many countries, Toxoplasma gondii (T. gondii) is a major cause of reproductive disorders and abortions in the sheep industry, and therefore responsible for important financial and economic losses. In addition, undercooked infected lamb is an important risk factor for human toxoplasmosis.In the present study, the initial phase of the infection was investigated: the parasite’s entry site, the subsequent distribution of the parasite and the host-immune response.ResultsParasite DNA was already detected in the cranial small intestinal mucosa the first days after oral infection with T. gondii tissue cysts. Simultaneously, high IFN-gamma and IL-12 responses were induced mainly in the mesenteric lymph nodes. The emergence of IgG1 (at 8dpi), and IgG2 (at 11 dpi) was accompanied by a decrease or even disappearance of the IFN-gamma and IL-12 response in the Peyers’ patches (PP), PBMC’s and popliteal LN’s. Meanwhile the parasite DNA could be recovered from most mucosal and systemic tissues to become undetectable in the small intestine, popliteal LN, PBMC and spleen 3 weeks pi.ConclusionsOur results indicate that parasites enter the cranial small intestine the first days after infection and that after an increase the first two weeks after infection, the parasite DNA levels in the intestine drop below the detection limit three weeks after infection. This coincides with an increase in parastic-specific serum IgG1 and IgG2 and a decrease of the antigen-specific IFN-gamma response in PP, PBMC and popliteal LN. We suggest a role for IFN-gamma and IL-12 in controlling the infection.

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Th1 and Th2 immune response to P30 and ROP18 peptides in human toxoplasmosis

Cited by 44 publications

References 24 publications

Genetic Polymorphisms in Cytokine Genes in Colombian Patients with Ocular Toxoplasmosis

Genetic Polymorphisms in Cytokine Genes in Colombian Patients with Ocular Toxoplasmosis

An overview of seventy years of research (1944 – 2014) on toxoplasmosis in Colombia, South America

Parasite distribution and associated immune response during the acute phase of Toxoplasma gondiiinfection in sheep

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