Th1 and Th2 Cytokines Activity during Transformation and Lymphoma Formation Stage in Chicken Naturally Infected with Marek's disease virus.

Abd-Ellatieff, Hoda A.; Rawash, A. A. Abou

doi:10.9790/2380-1001017280

Cited by 3 publications

(1 citation statement)

References 36 publications

(27 reference statements)

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“…MDV, which belongs to Alphaherpesvirinae subfamily, affects T-lymphocyte and causes nervous symptoms and ocular lesions [ 2 ]. REV, which belongs to the gammaretrovirus genus, causes bursal tumors by affecting pre-B and pre-T lymphocytes.…”

Section: Introductionmentioning

confidence: 99%

Genetic variability of the Avian leukosis virus subgroup J gp85 gene in layer flocks in Lower Egypt

et al. 2020

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Aim: This study aimed to determine the prevalence of layer flock tumor disease in Lower Egypt during the period of 2018- 2019 and to undertake molecular characterization and determine the genetic diversity of all identified viruses. Materials and Methods: Forty samples were collected from layer chicken located in six governorates of Lower Egypt during the period of 2018-2019. Samples were taken from tumors in different organs. Tumor tissues were identified by histopathological sectioning and then further confirmed by a reverse-transcription polymerase chain reaction. Finally, genetic evolution of Avian leukosis virus (ALV-J) gp85 gene was studied. Results: All the study samples were negative for Marek's disease virus, reticuloendotheliosis virus A,B,C and D and 20 samples were positive for ALV-J in backyard in six governrates. Sequencing of ALV-J gp85 gene was performed for six representative samples (one from each governorate), and they were found to be genetically related to prototype virus HPRS-1003 (identity percentage: 91.2-91.8%), but they were from a different group that was similar to the AF88-USA strain (first detected in 2000) with specific mutations, and they differed from a strain that was previously isolated in Egypt in 2005, forming two different subgroups (I and II) that had mutations in the hr1domain (V128F, R136A) and hr2 domain (S197G, E202K). Conclusion: The ALV-J virus was the main cause of neoplastic disease in layer chickens from Lower Egypt in the period of 2018-2019. We found that the genetic evolution of ALV-J gp85 gene was related to prototype virus HPRS-1003 but in a different group with a specific mutation. Further studies are needed to evaluate the antigenicity and pathogenicity of recently detected ALV-J strains.

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Section: Introductionmentioning

confidence: 99%

Genetic variability of the Avian leukosis virus subgroup J gp85 gene in layer flocks in Lower Egypt

et al. 2020

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Molecular characterisation and phylogenetic analysis of Marek’s disease virus in Turkish layer chickens

Yilmaz

Turan

Bayraktar

et al. 2020

British Poultry Science

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Molecular characterization and phylogenetic analysis of a virulent Marek’s disease virus field strain in broiler chickens in Japan

et al. 2017

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Marek's disease is a lymphoproliferative disease causing a serious threat in poultry production. Field strains of Marek's disease virus (MDVs) are continuously re-emerging, causing great economical losses to the poultry industry worldwide in spite of the intensive vaccination and restrictive management policy used. Histopathological and molecular characterizations of MDVs are essential for monitoring the changes of viruses and evaluating the effectiveness of existing vaccines. During 2016, 190 visceral tumour tissues representing 30 vaccinated chicken flocks from the Gifu prefecture, Japan, were analysed. A pathological examination revealed the presence of lymphoproliferative lesions in the visceral organs. Polymerase chain reaction screening of tissue specimens using specific primers for avian leucosis virus, reticuloendotheliosis virus, and MDV was positive only for MDV. The polymerase chain reaction products of meq, pp38, virus-induced IL-8 homology, and glycoprotein MDV genes were sequenced and used for homology, phylogenetic, and similarity level analysis with the published reference of MDVs in the database. The results revealed high similarity between the field isolates, vv and vv+ strains of MDV from the USA and China. Several point mutations in the nucleotide sequence of the field isolates and their deduced amino acid sequences were detected in those genes. The present molecular analyses indicated that nucleotide and amino acid changes could be valuable criteria for differentiation and determination of the pathogenicity and oncogenicity of MDVs according to the Avian Disease and Oncology Laboratory pathotyping in vivo studies. Furthermore, the results suggest that development of a new vaccine must be considered to overcome this devastating avian oncogenic viral disease.

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Th1 and Th2 Cytokines Activity during Transformation and Lymphoma Formation Stage in Chicken Naturally Infected with Marek's disease virus.

Cited by 3 publications

References 36 publications

Genetic variability of the Avian leukosis virus subgroup J gp85 gene in layer flocks in Lower Egypt

Genetic variability of the Avian leukosis virus subgroup J gp85 gene in layer flocks in Lower Egypt

Molecular characterisation and phylogenetic analysis of Marek’s disease virus in Turkish layer chickens

Molecular characterization and phylogenetic analysis of a virulent Marek’s disease virus field strain in broiler chickens in Japan

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