TGP attenuates endoplasmic reticulum stress and regulates the expression of thioredoxin-interacting protein in the kidneys of diabetic rats

Shao, Yunxia; Qi, Xiangming; Xu, Xin-Xing; Wang, Kun; Wu, Yuangang; Xia, Lingling

doi:10.5582/bst.2016.01188

Cited by 6 publications

(3 citation statements)

References 37 publications

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“…The total glucosides of paeony (TGP), an active ingredient derived from the root of Paeonia alba , possess anti-inflammatory, anti-apoptotic, antioxidant, and immunomodulatory pharmacological properties ( Jin and Zhang, 2022 ). Shao et al (2017) indicated that TGP significantly diminished urinary protein in diabetic rats, an effect linked to its inhibition of ER stress-related markers and TXNIP expression.…”

Section: Modulatory Role Of Chinese Herbal Medicine In Er Stress In D...mentioning

confidence: 97%

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The role of Chinese herbal medicine in the treatment of diabetic nephropathy by regulating endoplasmic reticulum stress

Wei

Liu

et al. 2023

Front. Pharmacol.

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Diabetic nephropathy (DN), a prevalent microvascular complication of diabetes mellitus, is the primary contributor to end-stage renal disease in developed countries. Existing clinical interventions for DN encompass lifestyle modifications, blood glucose regulation, blood pressure reduction, lipid management, and avoidance of nephrotoxic medications. Despite these measures, a significant number of patients progress to end-stage renal disease, underscoring the need for additional therapeutic strategies. The endoplasmic reticulum (ER) stress response, a cellular defense mechanism in eukaryotic cells, has been implicated in DN pathogenesis. Moderate ER stress can enhance cell survival, whereas severe or prolonged ER stress may trigger apoptosis. As such, the role of ER stress in DN presents a potential avenue for therapeutic modulation. Chinese herbal medicine, a staple in Chinese healthcare, has emerged as a promising intervention for DN. Existing research suggests that some herbal remedies may confer renoprotective benefits through the modulation of ER stress. This review explores the involvement of ER stress in the pathogenesis of DN and the advancements in Chinese herbal medicine for ER stress regulation, aiming to inspire new clinical strategies for the prevention and management of DN.

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Section: Modulatory Role Of Chinese Herbal Medicine In Er Stress In D...mentioning

confidence: 97%

“…(Jin and Zhang, 2022). Shao et al (2017) indicated that TGP significantly diminished urinary protein in diabetic rats, an effect linked to its inhibition of ER stress-related markers and TXNIP expression.…”

Section: Lignansmentioning

confidence: 99%

The role of Chinese herbal medicine in the treatment of diabetic nephropathy by regulating endoplasmic reticulum stress

Wei

Liu

et al. 2023

Front. Pharmacol.

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“…Diabetes research has demonstrated an association between TNF-α [50-51] and ESR [52]. Apoptosis of VECs is merely the initial stage of cardiovascular disease, and the ERS signaling pathway plays an important role in the regulation of VEC dysfunction [53].…”

Section: Discussionmentioning

confidence: 99%

Effect of the Diabetic Environment On the Expression of MiRNAs in Endothelial Cells: Mir-149-5p Restoration Ameliorates the High Glucose-Induced Expression of TNF-α and ER Stress Markers

Yuan

Chen

et al. 2017

Cell Physiol Biochem

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Background/Aims: This study aimed to screen microRNAs and their corresponding target genes that are associated with vascular injury in type two diabetes mellitus (T2DM), investigate the effects of differentially expressed miRNAs and their target genes on high glucose-induced vascular injury and establish the mechanism underlying these effects. Methods: A high-throughput digital gene expression (DGE) sequencing was performed to sequence microRNAs (miRNAs) and messenger RNAs (mRNAs) and determine their differential expression in human umbilical vein endothelial cells (HUVECs) incubated with serum samples from patients with T2DM and healthy volunteers. The HUVECs were transfected with si-TNF-α (tumor necrosis factor α) and a miR-149-5p inhibitor or mimic in vitro and then treated with normal or high glucose. The relative content of nitric oxide (NO) in the cells was detected using the Griess Reagent System. The mRNA and protein expression of endothelial nitric oxide synthase (eNOS) were determined by qRT-PCR and Western blotting. The content of endothelin-1 (ET-1), von Willebrand factor (vWF), and intercellular adhesion molecular-1 (ICAM-1) were detected using an enzyme-linked immunosorbent assay (ELISA) kit. Apoptosis was determined by flow cytometry using the Annexin V/PI apoptosis detection kit. The mRNA and protein expression levels of ER stress (ERS) markers were determined by qRT-PCR and Western blotting. Results: Based on the high-energy sequencing and in vitro pre-experiment studies, we determined that miR-149-5p and TNF-α were a differentially expressed mRNA/miRNA pair in T2DM with vascular injury. The luciferase reporter assay results demonstrated that miR-149-5p could directly target TNF-α. The upregulation of miR-149-5p reduced the high glucose-induced dysfunction in the HUVECs by significantly decreasing the levels of ET-1, vWF, and ICAM-1 and increasing the level of NO and the expression of eNOS. Additionally, we found that miR-149-5p can improve cell injury and reduce apoptosis by restoring the ameliorated high glucose-induced expression of ERS markers. Conclusion: TNF-α and miR-149-5p were differentially expressed in T2DM vascular endothelial injury. The over-expression of miR-149-5p ameliorates the high glucose-induced injury in the HUVECs by regulating the expression of TNF-α and ERS markers.

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Endoplasmic reticulum stress mechanisms and exercise intervention in type 2 diabetes mellitus

Chen,

Zhao,

et al. 2024

Biomedicine & Pharmacotherapy

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TGP attenuates endoplasmic reticulum stress and regulates the expression of thioredoxin-interacting protein in the kidneys of diabetic rats

Cited by 6 publications

References 37 publications

The role of Chinese herbal medicine in the treatment of diabetic nephropathy by regulating endoplasmic reticulum stress

The role of Chinese herbal medicine in the treatment of diabetic nephropathy by regulating endoplasmic reticulum stress

Effect of the Diabetic Environment On the Expression of MiRNAs in Endothelial Cells: Mir-149-5p Restoration Ameliorates the High Glucose-Induced Expression of TNF-α and ER Stress Markers

Endoplasmic reticulum stress mechanisms and exercise intervention in type 2 diabetes mellitus

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