TGN38 is required for the metaphase I/anaphase I transition and asymmetric cell division during mouse oocyte meiotic maturation

Chen, Lei; Ge, Zhao‐Jia; Wang, Zhenbo; Sun, Tianyi; Ouyang, Ying‐Chun; Sun, Qing‐Yuan; Sun, Yingpu

doi:10.4161/15384101.2015.945828

Cited by 7 publications

(5 citation statements)

References 38 publications

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“…However, our results identified MAP1B mRNA expression in porcine oocytes before and after IVM. The role of microtubule formation during nuclear and cytoplasmic maturation in mammalian oocytes, particularly in mouse, bovine, and pigs, is well recognized (Chen et al ., 2014; Zhang et al ., 2014; Jeon et al ., 2015; Mahdipour et al ., 2015; Solc et al ., 2015). However, most results are related to the oocyte's cytoskeleton, centrosome, or microtubule modifications during cell cycle progression and chromosome segregation during meiotic oocyte maturation.…”

Section: Discussionmentioning

confidence: 99%

Morphogenesis-related gene-expression profile in porcine oocytes before and after in vitro maturation

Budna

Chachuła

Kaźmierczak

et al. 2017

Zygote

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Mammalian oocyte maturation is achieved when oocytes reach metaphase II (MII) stage, and accumulate mRNA and proteins in the cytoplasm following fertilization. It has been shown that oocytes investigated before and after in vitro maturation (IVM) differ significantly in transcriptomic and proteomic profiles. Additionally, folliculogenesis and oogenesis is accompanied by morphogenetic changes, which significantly influence further zygote formation and embryo growth. This study aimed to determine new transcriptomic markers of porcine oocyte morphogenesis that are associated with cell maturation competence. An Affymetrix microarray assay was performed on an RNA template isolated from porcine oocytes before (n = 150) and after (n = 150) IVM. The brilliant cresyl blue (BCB) staining test was used for identification of cells with the highest developmental capacity. DAVID (Database for Annotation, Visualization, and Integrated Discovery) software was used for the extraction of the genes belonging to a cell morphogenesis Gene Ontology group. The control group consisted of freshly isolated oocytes. In total, 12,000 different transcripts were analysed, from which 379 genes were downregulated and 40 were upregulated in oocytes following IVM. We found five genes, SOX9, MAP1B, DAB2, FN1, and CXCL12, that were significantly upregulated in oocytes after IVM (in vitro group) compared with oocytes analysed before IVM (in vivo group). In conclusion, we found new transcriptomic markers of oocyte morphogenesis, which may be also recognized as significant mediators of cellular maturation capacity in pigs. Genes SOX9, MAP1B, DAB2, FN1, and CXCL12 may be involved in the regulation of the MII stage oocyte formation and several other processes that are crucial for porcine reproductive competence.

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Section: Discussionmentioning

confidence: 99%

Morphogenesis-related gene-expression profile in porcine oocytes before and after in vitro maturation

Budna

Chachuła

Kaźmierczak

et al. 2017

Zygote

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“…Microinjection of mRNAs and siRNAs were performed as previously described. 51,52 The corresponding mRNA concentration was 0.1 mg/ml for protein localization and 1 mg/ml for protein overexpression. The same amount of RNase-free water was injected as control.…”

Section: Microinjection Of Mrna and Sirnamentioning

confidence: 99%

Casein kinase 1 (α, δ and ε) localize at the spindle poles, but may not be essential for mammalian oocyte meiotic progression

Wang

Huang³

et al. 2015

Cell Cycle

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CK1 (casein kinase 1) is a family of serine/threonine protein kinase that is ubiquitously expressed in eukaryotic organism. CK1 members are involved in the regulation of many cellular processes. Particularly, CK1 was reported to phosphorylate Rec8 subunits of cohesin complex and regulate chromosome segregation in meiosis in budding yeast and fission yeast. (1-3) Here we investigated the expression, subcellular localization and potential functions of CK1α, CK1δ and CK1ε during mouse oocyte meiotic maturation. We found that CK1α, CK1δ and CK1ε all concentrated at the spindle poles and co-localized with γ-tubulin in oocytes at both metaphase I (MI) and metaphase II (MII) stages. However, depletion of CK1 by RNAi or overexpression of wild type or kinase-dead CK1 showed no effects on either spindle organization or chromosome segregation during oocyte meiotic maturation. Thus, CK1 is not the kinase that phosphorylates Rec8 cohesin in mammalian oocytes, and CK1 may not be essential for spindle organization and meiotic progression although they localize at spindle poles.

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“…The trans-Golgi network (TGN) is part of the secretory pathway of eukaryotic cells which is distinct from the Golgi stack and important in the later stages of protein secretion. Trans-Golgi network 38 (TGN38) is an integral membrane protein and predominantly localized to the TGN in NRK cells [6]. Interestingly, a few reports have implicated that ZnT7 may be involved in the regulation of insulin and glucose metabolism.…”

Section: Introductionmentioning

confidence: 99%

Protective Effect of Znt7 on High Glucose-Induced Epithelial-to-Mesenchymal Transition in Renal Tubular Epithelial Cells

Zhang

Liang³

et al. 2018

Kidney Blood Press Res

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Background/Aims: Evidence from our and other groups has demonstrated that zinc transporter 7 in SLC30 family (ZnT7) inhibited epithelial-to-mesenchymal transition (EMT) and apoptosis in rat peritoneal mesothelial cells (RPMCs) under high glucose (HG) concentration. In the present study, we investigated the effect of ZnT7 on EMT of renal tubular epithelial cells (RTECs) in an in vitro model of diabetic nephropathy (DN). Methods: A dual-fluorescent staining protocol was used for detection of ZnT7 in a normal rat kidney tubular epithelial cell line (NRK-52E cells). EMT was induced with HG (30 mM). NRK-52E cells were transfected with plasmids codifying for hZnT7-EGFP and interfering RNA for determination of the effect of ZnT7 over-expression and silencing, respectively. Expression of ZnT7, activation of the MAPK/ERK and TGF-β/Smad pathways were analyzed with by means of Western blot. Results: ZnT7 was localized in the perinuclear region and Golgi apparatus. In HG-induced EMT of NRK-52E cells, ZnT7 was up-regulated. Over-expression of ZnT7 led to inhibition of HG-induced EMT, while knock-down of ZnT7 increased EMT. Furthermore, knock-down of ZnT7 and increased HG-induced EMT was accompanied by activation of the MAPK/ERK and TGF-β/Smad pathways. Conclusion: The present study provides evidence that ZnT7 has a protective effect over EMT of RTECs in DN and suggests that the inhibition of HG-induced EMT may be achieved through the MAPK/ERK and TGF-β/Smad pathways. Thereby, ZnT7 could be a potential target for translation medicine and prevention program in DN.

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TGN38 is required for the metaphase I/anaphase I transition and asymmetric cell division during mouse oocyte meiotic maturation

Cited by 7 publications

References 38 publications

Morphogenesis-related gene-expression profile in porcine oocytes before and after in vitro maturation

Morphogenesis-related gene-expression profile in porcine oocytes before and after in vitro maturation

Casein kinase 1 (α, δ and ε) localize at the spindle poles, but may not be essential for mammalian oocyte meiotic progression

Protective Effect of Znt7 on High Glucose-Induced Epithelial-to-Mesenchymal Transition in Renal Tubular Epithelial Cells

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