Tgfbr1 maps to Chromosome 4

Analysis of loss of heterozygosity in lymphoma and leukaemia arising in F1 hybrid mice locates a common region of chromosome 4 loss

“…The type I receptor for the transforming growth factor gene, Tgfbr1, has been mapped by Kuan and Kono (1998) to proximal chromosome 4 between D4Mit4 (16.4 cM, MIT F2 intercross) and D4Mit217 (30.6 cM, MIT F2 intercross). Unfortu-nately, the PCR polymorphism for Tgfbr1 was not informative for the F1 hybrid mice studied here.…”

Loss of heterozygosity at the proximal-mid part of mouse chromosome 4 defines two novel tumor suppressor gene loci in T-cell lymphomas

“…Such a pattern has been observed on mouse chromosome 4 in dierent types of primary tumors, including lung cancers induced by vinyl carbamate (Herzog et al, 1994(Herzog et al, , 1995 or by 4-methylnitrosamino-1-3-pyridyl-1-butanone (Hegi et al, 1994), 2',3'-dideoxycytidine-and 1,3-butadiene-induced primary lymphomas (Zhuang et al, 1996), and mammary tumors induced by medroxyprogesterone acetate and 7,12-dimethylbenzoanthracene (Aldaz et al, 1996). In addition, primary tumors arising in mice transgenic for the mammary tumor virus /v-Ha-ras (Radany et al, 1997) and for the neu proto-oncogene (Ritland et al, 1997), as well as in cell lines derived from hepatocarcinomas (Lee et al, 1995;Miyasaka et al, 1995), also revealed frequent loss of heterozygosity (LOH) on mouse chromosome 4. Recent studies in our laboratory reported a high frequency of allelic losses on this chromosome in g-radiation-induced T-cell lymphomas of F1 hybrid mice (Santos et al, 1996(Santos et al, , 1998.…”

“…These results and the existence of tumors with LOH occurring exclusively at D4Mit21, clearly suggest that another dierent thymic lymphoma suppressor region (TLSR5) may be de®ned on the proximal-mid part of chromosome 4. Interestingly, the D4Mit21 marker lies within the interval de®ned by D4Mit4 (12.1 cM) and D4Mit217 (28.6 cM), where Tgfbr1 (type I receptor for TGF-b) gene was mapped (Kuan and Kono, 1998), and TGF-b can induce the transcriptional activation of p15 INK4b (Hannon and Beach, 1994). The orthologous human type I receptor for TGF-b (TGFBR1) was initially mapped to the chromosome band 9q33 ± 34 (Johnson et al, 1995), where frequent allelic losses were found in several types of tumors (Miura et al, 1995;Schultz et al, 1995;Simoneau et al, 1996;Takita et al, 1997).…”

“…All this evidence supports the notion that Tgfbr1 is a good candidate tumor suppressor gene for TLSR5. To determine the possible involvement of this gene in T-cell lymphomagenesis, we intended to perform a LOH analysis using a PCR-RL polymorphism described by Kuan and Kono (1998). Unfortunately, this marker was not informative in our F1 hybrid mice.…”