TGF-β1 suppresses de novo cholesterol biosynthesis in granulosa-lutein cells by down-regulating DHCR24 expression via the GSK-3β/EZH2/H3K27me3 signaling pathway

Li, Hui; Shen, Jie; Ma, Shinan; Zhao, Fang; Zhao, Weimin; Chen, Fang; Fu, Yanfeng; Li, Bixia; Cheng, Jinhua; Deng, Yanfei

doi:10.1016/j.ijbiomac.2022.10.196

Cited by 5 publications

(1 citation statement)

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“…The TGF-β signaling pathway is important in mammals, and studies have shown that TGF-β1 mediates MMP1 down-regulation of type I collagen deposition in granulosa cells through the AKT/GSK-3β signaling pathway, which inhibits luteinization and progesterone production in granulosa cells [ 34 ]. It also down-regulates DHCR24 expression via the GSK-3β/EZH2/H3K27me3 signaling pathway, which in turn inhibits cholesterol denovo synthesis in granulosa-lutein cells [ 35 ]. TGF-β1/SMAD4/miR-183-96-182/FoxO1 may be one of the pathways regulating follicular atresia and female reproduction [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals Differentially Expressed circRNAs Associated with Fecundity in Small-Tail Han Sheep Thyroid with Different FecB Genotypes

Chang,

He,

et al. 2023

Animals

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Litter size is an economically important trait in sheep, and it is a complex trait controlled by multiple genes in multiple organs. Among them, the regulation of lamb number trait by the thyroid gland is a very important part. However, the molecular mechanisms of the thyroid gland in sheep reproduction remain unclear. Here, RNA-seq was used to detect transcriptome expression patterns in the thyroid gland between follicular phase (FP) and luteal phase (LP) in FecB BB (MM) and FecB ++ (ww) STH sheep, respectively, and to identify differentially expressed circRNAs (DECs) associated with reproduction. Bioinformatic analysis of the source genes of these DECs revealed that they can be enriched in multiple signaling pathways involved in the reproductive process of animals. We found that the source genes of these DECs, such as GNAQ, VEGFC, MAPK1, STAT1, and HSD17B7, may play important roles in the reproductive process of animals. To better understand the function of these DECs, we constructed circRNA–miRNA co-expression networks. Dual luciferase reporter assays suggested that a ceRNA regulatory mechanism between circ_0003259-oar-miR-133-TXLNA and circ_0012128-oar-miR-370-3p-FGFR1 may hold. All of these DEC expression profiles in the thyroid gland provide a novel resource for elucidating the regulatory mechanisms underlying STH sheep prolificacy.

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Section: Discussionmentioning

confidence: 99%