TGF-β1 induces type I collagen deposition in granulosa cells via the AKT/GSK-3β signaling pathway-mediated MMP1 down-regulation

Shen, Jie; Wang, Zeping; Zhao, Weimin; Fu, Yanfeng; Li, Bixia; Cheng, Jinhua; Deng, Yanfei; Li, Saijiao; Li, Hui

doi:10.1016/j.repbio.2022.100705

Cited by 4 publications

(3 citation statements)

References 43 publications

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“…KEGG analysis revealed that these genes could be enriched in the TGF-β signaling pathway, p53 signaling pathway, GnRH signaling pathway, NF-κB signaling pathway, ovarian steroidogenesis, and other signaling pathways related to the animal reproductive process. The TGF-β signaling pathway is important in mammals, and studies have shown that TGF-β1 mediates MMP1 down-regulation of type I collagen deposition in granulosa cells through the AKT/GSK-3β signaling pathway, which inhibits luteinization and progesterone production in granulosa cells [ 34 ]. It also down-regulates DHCR24 expression via the GSK-3β/EZH2/H3K27me3 signaling pathway, which in turn inhibits cholesterol denovo synthesis in granulosa-lutein cells [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals Differentially Expressed circRNAs Associated with Fecundity in Small-Tail Han Sheep Thyroid with Different FecB Genotypes

Chang,

He,

et al. 2023

Animals

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Litter size is an economically important trait in sheep, and it is a complex trait controlled by multiple genes in multiple organs. Among them, the regulation of lamb number trait by the thyroid gland is a very important part. However, the molecular mechanisms of the thyroid gland in sheep reproduction remain unclear. Here, RNA-seq was used to detect transcriptome expression patterns in the thyroid gland between follicular phase (FP) and luteal phase (LP) in FecB BB (MM) and FecB ++ (ww) STH sheep, respectively, and to identify differentially expressed circRNAs (DECs) associated with reproduction. Bioinformatic analysis of the source genes of these DECs revealed that they can be enriched in multiple signaling pathways involved in the reproductive process of animals. We found that the source genes of these DECs, such as GNAQ, VEGFC, MAPK1, STAT1, and HSD17B7, may play important roles in the reproductive process of animals. To better understand the function of these DECs, we constructed circRNA–miRNA co-expression networks. Dual luciferase reporter assays suggested that a ceRNA regulatory mechanism between circ_0003259-oar-miR-133-TXLNA and circ_0012128-oar-miR-370-3p-FGFR1 may hold. All of these DEC expression profiles in the thyroid gland provide a novel resource for elucidating the regulatory mechanisms underlying STH sheep prolificacy.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals Differentially Expressed circRNAs Associated with Fecundity in Small-Tail Han Sheep Thyroid with Different FecB Genotypes

Chang,

He,

et al. 2023

Animals

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“…These findings indicate that FBN1 plays an important role in follicular development together with the interacting proteins in these species. losa cells and cell markers for theca interna and granulosa cells [22][23][24]. Otherwise, the predicted protein network map of human FBN1 provided by the BioGRID database showed that KIAA1429, which is related with follicular development, has the high correlation with FBN1 besides the above interacting proteins (Figure 3E).…”

Section: Protein Structure and Interaction Network Analysis On Fbn1mentioning

confidence: 97%

“…In addition, Homo sapiens would also been involved in the protein interaction network analysis because there are many similarities in the reproduction mechanism between humans and sheep (bicorned uterus and similar ovulation rate) and in-depth function studies have been carried out on FBN1 protein in Homo sapiens [20,21]. Protein interaction network analysis for Capra hircus, Homo sapiens and Bos taurus demonstrated the 10 proteins most closely interacting with FBN1 (Figure 3B-D), and, among them, the five proteins (ELN, LUM, MFAP5, COL1A2, COL5A2) were associated with the proliferation of ovarian granulosa cells and cell markers for theca interna and granulosa cells [22][23][24]. Otherwise, the predicted protein network map of human FBN1 provided by the BioGRID database showed that KIAA1429, which is related with follicular development, has the high correlation with FBN1 besides the above interacting proteins (Figure 3E).…”

Section: Protein Structure and Interaction Network Analysis On Fbn1mentioning

confidence: 99%

Association Analyses between Single Nucleotide Polymorphisms in ZFAT, FBN1, FAM184B Genes and Litter Size of Xinggao Mutton Sheep

Gong,

Chen,

et al. 2023

Animals

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Previous studies have screened key candidate genes for litter size in sheep, including fibrillin-1 (FBN1), family with sequence similarity 184 member B (FAM184B) and zinc finger and AT-hook domain containing (ZFAT). Therefore, it is necessary to verify these genes in the Xinggao mutton sheep population and determine the associated loci for litter size. In this study, three loci (FBN1 g.160338382 T > C, FAM184B g.398531673 C > T and ZFAT g.20150315 C > T) were firstly screened based on the population differentiation coefficient between the polytocous and monotocous sheep groups. Then, population genetic analysis and association analysis were performed on these loci. The results revealed that the g.160338382 T > C in FBN1 was significantly associated with the litter size of sheep. Moreover, there was no significant interaction effect between the g.160338382 T > C locus and FecB on litter size. Notably, g.160338382 T > C is adjacent to the anterior border of exon 58 and belongs to a splice polypyrimidine tract variant, which may lead to alternative splicing and ultimately cause changes in the structure and function of the protein. In summary, our results provided a potentially effective genetic marker for improving the litter size of sheep.

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Extracellular vesicles secreted by cumulus cells contain microRNAs that are potential regulatory factors of mouse oocyte developmental competence

Fiorentino,

Merico,

Zanoni

et al. 2024

Molecular Human Reproduction

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The role of cumulus cells (CCs) in the acquisition of oocyte developmental competence is not yet fully understood. In a previous study, we matured cumulus-denuded fully-grown mouse oocytes to metaphase II (MII) on a feeder layer of CCs (FL-CCs) isolated from developmentally competent (FL-SN-CCs) or incompetent (FL-NSN-CCs) SN (surrounded nucleolus) or NSN (not surrounding nucleolus) oocytes, respectively. We observed that oocytes cultured on the former could develop into blastocyst, while those matured on the latter arrested at the 2-cell stage. To investigate the CC factors contributing to oocyte developmental competence, here we focused on the CCs release into the medium of extracellular vesicles (EVs) and on their miRNA content. We found that, during the 15-hr transition to MII, both FL-SN-CCs and FL-NSN-CCs release EVs that can be detected, by confocal microscopy, inside the zona pellucida (ZP) or the ooplasm. The majority of EVs are <200 nm in size, which is compatible with their ability to cross the ZP. Next-generation sequencing of the miRNome of FL-SN-CC versus FL-NSN-CC EVs highlighted 74 differentially expressed miRNAs, with 43 up- and 31 down-regulated. Although most of these miRNAs do not have known roles in the ovary, in silico functional analysis showed that seven of these miRNAs regulate 71 target genes with specific roles in meiosis resumption (N = 24), follicle growth (N = 23), fertilisation (N = 1) and the acquisition of oocyte developmental competence (N = 23). Overall, our results indicate CC EVs as emerging candidates of the CC-to-oocyte communication axis and uncover a group of miRNAs as potential regulatory factors.

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TGF-β1 induces type I collagen deposition in granulosa cells via the AKT/GSK-3β signaling pathway-mediated MMP1 down-regulation

Cited by 4 publications

References 43 publications

Transcriptome Analysis Reveals Differentially Expressed circRNAs Associated with Fecundity in Small-Tail Han Sheep Thyroid with Different FecB Genotypes

Transcriptome Analysis Reveals Differentially Expressed circRNAs Associated with Fecundity in Small-Tail Han Sheep Thyroid with Different FecB Genotypes

Association Analyses between Single Nucleotide Polymorphisms in ZFAT, FBN1, FAM184B Genes and Litter Size of Xinggao Mutton Sheep

Extracellular vesicles secreted by cumulus cells contain microRNAs that are potential regulatory factors of mouse oocyte developmental competence

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