TFIIH: A multi-subunit complex at the cross-roads of transcription and DNA repair

Kolesnikova, Olga; Radu, Laura; Poterszman, Arnaud

doi:10.1016/bs.apcsb.2019.01.003

Cited by 42 publications

(34 citation statements)

References 220 publications

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“…Arabidopsis CDKD;1–3 are closely related homologues of budding yeast Kin28, fission yeast Msc6 and metazoan CDK7 protein kinases, which together with cyclin H and the MAT1 (Menage a trois 1) assembly factor form the trimeric kinase module (TFIIK) of general transcription factor TFIIH (Rimel and Taatjes, ; Kolesnikova et al ., ). When unbound of TFIIH, TFIIK plays a pivotal role in cell cycle control through activation of cell cycle kinases by T‐loop phosphorylation.…”

Section: Discussionmentioning

confidence: 97%

“…In budding yeast, Cak1 is not part of the TFIIH (Kaldis et al ., ) but nonetheless remains associated with RNAPII and mediates T‐loop phosphorylation and activation of Kin28, Bur1 and Ctk1 RNAPII CTD kinases (Espinoza et al ., ; Kimmelman et al ., ; Yao and Prelich, ; Ostapenko and Solomon, ). During PIC formation, TFIIH mediates ATP hydrolysis‐dependent opening of promoter region around the transcription start site (TSS) (Rimel and Taatjes, ; Kolesnikova et al ., ). In the horseshoe‐like structure of TFIIH (Figure ), which is recruited by the Mediator to PIC, the terminal XPB and XPD DNA helicases interact with each other and the MAT1 subunit of kinase module (Greber et al ., ).…”

Section: Discussionmentioning

confidence: 97%

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Gene modification by fast‐track recombineering for cellular localization and isolation of components of plant protein complexes

Ghosh

Stolze

et al. 2019

The Plant Journal

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To accelerate the isolation of plant protein complexes and study cellular localization and interaction of their components, an improved recombineering protocol is described for simple and fast site-directed modification of plant genes in bacterial artificial chromosomes (BACs). Coding sequences of fluorescent and affinity tags were inserted into genes and transferred together with flanking genomic sequences of desired size by recombination into Agrobacterium plant transformation vectors using three steps of E. coli transformation with PCR-amplified DNA fragments. Application of fast-track recombineering is illustrated by the simultaneous labelling of CYCLIN-DEPENDENT KINASE D (CDKD) and CYCLIN H (CYCH) subunits of kinase module of TFIIH general transcription factor and the CDKD-activating CDKF;1 kinase with green fluorescent protein (GFP) and mCherry (green and red fluorescent protein) tags, and a PIPL (His 18 -StrepII-HA) epitope. Functionality of modified CDKF;1 gene constructs is verified by complementation of corresponding T-DNA insertion mutation. Interaction of CYCH with all three known CDKD homologues is confirmed by their co-localization and co-immunoprecipitation. Affinity purification and mass spectrometry analyses of CDKD;2, CYCH, and DNA-replication-coupled HISTONE H3.1 validate their association with conserved TFIIH subunits and components of CHROMATIN ASSEMBLY FACTOR 1, respectively. The results document that simple modification of plant gene products with suitable tags by fast-track recombineering is well suited to promote a wide range of protein interaction and proteomics studies.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

Gene modification by fast‐track recombineering for cellular localization and isolation of components of plant protein complexes

Ghosh

Stolze

et al. 2019

The Plant Journal

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“…The XPG gene locating at chromosome 13q33 consists of 15 exons[10], which is considered to cut the DNA at the 3’ terminus, initiate transcription-coupled DNA repair, and participate in RNA polymerase II transcription[32]. In the process of DNA repair, XPG binds to XPB as part of the transcription factor IIH (TFIIH) complex and strongly interacts with the TFIIH complex, a multi-subunit complex located at the intersection of transcription and DNA repair[33], which is involved in the DNA demethylation induced by overexpression of Gadd45a[10]. Meanwhile, XPG-related nucleases are used hierarchically for the excision of double-stranded rDNA break resection[34].…”

Section: Discussionmentioning

confidence: 99%

Association of XPG rs2094258 polymorphism with gastric cancer prognosis

Wang¹,

Terry²,

Yang³

et al. 2019

WJG

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BACKGROUNDThe xeroderma pigmentosum group G (XPG) gene at chromosome 13q33 consists of 15 exons, which may be related to the occurrence and development of gastric cancer (GC).AIMTo examine the association of several common single nucleotide polymorphisms (SNPs) of the XPG gene with GC risk and survival.METHODSFive SNPs of XPG (rs2094258, rs751402, rs873601, rs2296147, and rs1047768) were genotyped by PCR restriction fragment length polymorphism in 956 histologically confirmed GC cases and 1012 controls in North China. GC patients were followed for survival status and, if deceased, cause of death. Logistic regression and Cox regression were used for analysing associations of XPG SNPs with risk of GC and prognosis, respectively. For rs2094258, heterozygous model (CT vs CC), homozygous model (TT vs CC), recessive model (TT vs CT + CC), and dominant model (TT + CT vs CC) were analyzed.RESULTSNone of the examined loci were statistically associated with GC risk, although rs2296147 was marginally associated with GC risk (P = 0.050). GC patients with the rs2094258 CT + CC genotype showed worse survival than those with the TT genotype (log-rank test, P = 0.028), and patients with the CC genotype had a tendency of unfavourable prognosis compared with those with the TT + CT genotype (log-rank test, P = 0.039). The increase in C alleles of rs2094258 [hazard ratio (HR) = 1.19, 95% confidence interval (CI): 1.02-1.45, P = 0.037] were associated with the long-term survival of GC cases. Other risk factors for survival included tumor differentiation (HR = 4.51, 95%CI: 1.99-8.23, P < 0.001), lymphovascular invasion (HR = 1.97, 95%CI: 1.44-3.01, P < 0.001), and use of chemotherapy (HR = 0.81, 95%CI: 0.63-0.98, P = 0.041).CONCLUSIONThe XPG rs2094258 polymorphism may be associated with overall survival in GC patients.

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“…1 Besides participating to this repair pathway, proteins implicated in NER are also involved in other DNA repair mechanisms and in DNAassociated processes such as transcription and chromatin architecture. [2][3][4] Genetic defects in NER lead to a broad variety of autosomal recessive overlapping diseases, namely Cerebro-Oculo-Facio-Skeletal syndrome (COFS), Cockayne syndrome (CS), Xeroderma Pigmentosum (XP), Trichothiodystrophy (TTD), and UV-sensitive syndrome (UVSS). [5][6][7][8] Patients with CS, COFS, combined XP-CS phenotype or TTD display progressive neurodegenerative symptoms of varying severity, which play a leading role for the prognosis of these patients, as their life expectancy is variably reduced depending on the severity of these symptoms.…”

Section: Introductionmentioning

confidence: 99%

Early‐onset nucleotide excision repair disorders with neurological impairment: Clues for early diagnosis and prognostic counseling

et al. 2020

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Nucleotide excision repair associated diseases comprise overlapping phenotypes and a wide range of outcomes. The early stages still remain under‐investigated and underdiagnosed, even although an early recognition of the first symptoms is of utmost importance for appropriate care and genetic counseling. We systematically collected clinical and molecular data from the literature and from newly diagnosed NER patients with neurological impairment, presenting clinical symptoms before the age of 12 months, including foetal cases. One hundred and eighty‐five patients were included, 13 with specific symptoms during foetal life. Arthrogryposis, microcephaly, cataracts, and skin anomalies are the most frequently reported signs in early subtypes. Non ERCC6/CSB or ERCC8/CSA genes are overrepresented compared to later onset cohorts: 19% patients of this cohort presented variants in ERCC1, ERCC2/XPD, ERCC3/XPB or ERCC5/XPG. ERCC5/XPG is even the most frequently involved gene in foetal cases (10/13 cases, [4/7 families]). In this cohort, the mutated gene, the age of onset, the type of disease, severe global developmental delay, IUGR and skin anomalies were associated with earlier death. This large survey focuses on specific symptoms that should attract the attention of clinicians towards early‐onset NER diagnosis in foetal and neonatal period, without waiting for the completeness of classical criteria.

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TFIIH: A multi-subunit complex at the cross-roads of transcription and DNA repair

Cited by 42 publications

References 220 publications

Gene modification by fast‐track recombineering for cellular localization and isolation of components of plant protein complexes

Gene modification by fast‐track recombineering for cellular localization and isolation of components of plant protein complexes

Association of XPG rs2094258 polymorphism with gastric cancer prognosis

Early‐onset nucleotide excision repair disorders with neurological impairment: Clues for early diagnosis and prognostic counseling

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