Texturized Soy Protein as an Alternative Low-Cost Media for Bacteria Cultivation

Abstract

Most of the culture media used in bacterial growth is composed of complex ingredients, increasing the value of the product. This makes its acquisition unavailable by educational institutions without sufficient funding, making even more difficult the practical teaching of microbiology. Therefore, the development of an alternative medium of simple composition and low-cost becomes necessary. This work aimed to use texturized soy protein (TSP) as a low cost culture medium that allows the bacterial growth. For the composition of the broths, concentrations between 0.5% and 10% were prepared. Thirty-eight bacteria, including important pathogens associated with food, were inoculated and the concentration of 7.5% TSP allowed the growth of 100% of the tested bacteria, with a production cost of approximately 86% and 68% lower than tryptic soy broth and agar, respectively. This work demonstrates that the use of a culture medium of easy acquisition and low cost is feasible and has good results.

Use of products of vegetable origin and waste from hortofruticulture for alternative culture media

“…The work demonstrated the viability of an easily formulated, low cost, and highly efficient culture medium (Cruz et al, 2020).…”

“…Agro-industrial waste has great diversity and has been shown to have potential for application in different areas, such as use as a source of fibers, polyphenols and bioactive compounds in general (Costa et al, 2020;Tagliani et al, 2019), organization and management of fruit and vegetable residues for human and animal consumption (Ramírez et al, 2020) and application in the development of alternative culture media (Cruz et al, 2020).…”

“…In developing countries, the high cost of culture media hampers practical microbiology classes and scientific research in institutions with insufficient financial resources (Uthayasooriyan et al, 2016;Jadhav et al, 2018;Cruz et al, 2020). Thus, some studies have noted that vegetable-based formulations maybe advantageous as alternative (non-commercial) culture media, since these substrates provide several nutrients, including proteins, essential for the growth of microorganisms .…”

Development of a Novel Selective Medium for Culture of Gram-negative Bacteria

“…It is also predicted that a large number of bacteria species in the environment also cannot be cultured due to the lack of appropriate media which are needed for their growth [6]. This is the main driving force behind the constant effort for the formulation and development of newer media for bacterial culture [8][9][10]. Such new media will allow scientists to grow and study currently un-culturable bacterial species Plants are rich in a wide variety of chemical compounds such as tannins, terpenoids, alkaloids, and avonoids which have been found to possess antimicrobial properties against wide variety of bacterial pathogens.…”

Development of a novel selective medium for culture of Gram-negative bacteria

“…It is also predicted that a large number of bacteria species in the environment also cannot be cultured due to the lack of appropriate media which are needed for their growth [ 6 ]. This is the main driving force behind the constant effort for the formulation and development of newer media for culture of bacteria [ 8 – 10 ]. Such new media will allow scientists to grow and study currently un-culturable bacterial species.…”