Text Mining Analysis of Tourism Consciousness of Aged People in Japan

Abstract

In order to understand the mechanism for maintaining life of animals based on the search of dynamics of bio-molecules, I have developed several sensitive and selective methods for their quantiˆcation. Using the methods of derivatization with the developed benzofurazan ‰uorogenic reagents (4-‰uoro-7-nitro-2,1,3-benzoxadiazole (NBD F), ammonium 7-‰uoro-2,1,3-benzoxadiazole 4-sulfonate (SBD F) and etc.) followed by high-performance liquid chromatography (HPLC)-‰uorescence detection, a certain kind of biological and clinical importances was demonstrated of chiral bio-molecules (D-amino acids, D-lactic acid and so on), peptides and proteins. The proposed method (derivati-zation with SBD F, isolation of the ‰uorescent proteins by two-dimensional HPLC, enzymatic digestion and identiˆca-tion of the altered proteins by HPLC mass spectrometry (MS)/MS with database-searching algorithm) for proteomics studies revieled the changed proteins in the islets of Langerhans of the dexamethazone-induced diabetic rats. An importance of catecholamine metabolism on the blood pressure regulation was also suggested by the method of HPLC chemiluminescence detection of catecholamines and their 3-O-methylmetabolites. A newˆeldnewˆeld of Analytical Chemistry, i.e., Bio-Analytical Chemistry, was also proposed.