Teucrium polium Complex with Molybdate Enhance Cultured Islets Secretory Function

Monfared, Seyed Sajad Mohseni Salehi; Pournourmohammadi, Shirin

doi:10.1007/s12011-009-8424-8

Cited by 14 publications

(8 citation statements)

References 25 publications

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“…TP aqueous extract supplemented rats had significant decrease in cornstarch induced acute hyperglycemia 45 min post starch intragastric administration (Kasabri et al, 2011). TP antidiabetic activity was further evaluated in animal models, and through gavage and oral administration of a hydroalcoholic and water extract of TP, insulin secretion levels increased in the circulating blood in rats (Esmaeili and Yazdanparast, 2004;Mohseni Salehi Monfared and Pournourmohammadi, 2010;Tabatabaie and Yazdanparast, 2017). Insulinotropic properties of TP extracts was attributed to the presence of apigenin (5-hydroxy-4', 7-dimethoxyflavone) existing only in methanolic, ethanolic, but not in aqueous/ethanolic and fractions (Mirghazanfari et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…T. polium ethanol/water extract fed-diabetic rats experienced significant reductions in serum glucose levels and blood insulin level was enhanced by almost 160% (Shahraki et al, 2007). Gavage and oral administration of a hydroalcoholic and water extract of TP increased insulin secretion from rat-isolated islets (Mohseni Salehi Monfared and Pournourmohammadi, 2010) and insulin levels in rats (Esmaeili and Yazdanparast, 2004 (Esmaeili and Yazdanparast, 2004). In addition, TP ethyl acetate extract decreased serum, liver, and muscle triglyceride content of sucroseinduced insulin resistance in rats (Mousavi et al, 2012).…”

Section: Advancement In Medicinal Plant Researchmentioning

confidence: 99%

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Teucrium polium extracts stimulate GLUT4 translocation to the plasma membrane in L6 muscle cells

Kadan¹,

Sasson²,

Abu‐Reziq³

et al. 2018

Adv Med Plant Res

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Teucrium polium L. (TP) is recommended by herbal and integrative practitioners for the treatment of diabetes. However, its mechanism of action in stimulating GLUT4 activity and translocation to the plasma membrane is still unknown. This in vitro study examined the chemical composition, cytotoxicity, and antidiabetic activity of three TP distinct extracts: water/ethanol (WTP), methanol (MTP), and hexane (HTP). The compositions of the TP extracts were determined by GC/MS. MTT and LDH assays were used to assess the toxicity of the extracts. The efficacies of the TP extracts in enhancing glucose transporter-4 (GLUT4) translocation to plasma membrane (PM) were tested in L6 muscle cells, stably expressing myctagged GLUT4 using the cell-ELISA test. GC/MS phytochemical analysis of MTP and HTP extracts revealed 10 compounds in each extract, and only palmitic acid was communal in these two extracts. WTP, MTP, and HTP extracts were safe up to 63, 63, and 250 µg/ml, respectively. The HTP extract was the most efficient in GLUT4 translocation enhancement, while the least efficient was the WTP extract. In addition, the HTP extract increased the GLUT4 translocation at 32 µg/ml by 2-and 3-fold relative to the control in the absence and presence of insulin, respectively. A similar result was obtained with the MTP extract at 63 µg/ml. In contrast, WTP extract in the presence of insulin had no effect and in the absence of insulin had slightly enhanced GLUT4 translocation. These findings indicate that TP antidiabetic activity is mediated in part by enhancing GLUT4 translocation to the PM in skeletal muscle.

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Section: Discussionmentioning

confidence: 99%

Section: Advancement In Medicinal Plant Researchmentioning

confidence: 99%

Teucrium polium extracts stimulate GLUT4 translocation to the plasma membrane in L6 muscle cells

Kadan¹,

Sasson²,

Abu‐Reziq³

et al. 2018

Adv Med Plant Res

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“…For instance, both are able to inactivate glycogen synthase (Thompson et al ., ) and stimulate glucose uptake in rat adipocytes in the presence of H 2 O 2 (Chaves et al ., ). In addition, they also enhance insulin secretion by means of increasing cultured islets' function (Mohseni Salehi Monfared & Pournourmohammadi, ). In spite of the fact that their glucose lowering mechanisms are alike in diabetic rats, we did not find any effect on the hypersecretion of insulin in fructose rats.…”

Section: Discussionmentioning

confidence: 99%

Sodium molybdate prevents hypertension and vascular prostanoid imbalance in fructose‐overloaded rats

Peredo

Andrade

Donoso

et al. 2013

Auton Autocoid Pharmacol

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(1) Fructose (F) overload produces elevated blood pressure (BP), hyperglycaemia, hypertriglyceridemia and insulin resistance, resembling human metabolic syndrome. Previously, we found altered vascular prostanoid (PR) production in this model. (2) Sodium molybdate (Mo), as well as sodium tungstate, causes insulin-like effects and normalizes plasma glucose levels in streptozotocin-treated diabetic rats. We studied the effects of Mo on BP, metabolic parameters and release of PR from the mesenteric vascular bed (MVB) in F-overloaded rats. (3) Four groups of male Sprague-Dawley rats were analysed: Control, tap water to drink; F, F solution 10% W/V to drink; CMo, Mo 100 mg kg day(-1) and FMo, both treatments. After 9 weeks, the animals were killed and MVBs removed and the released PRs measured. (4) F increased BP, glycemia, triglyceridemia and insulinemia. Mo treatment prevented the increases in BP and glycemia, but did not modify triglyceridemia or insulinemia. In addition, Mo decreased BP in controls. (5) Prostaglandins (PG) F2 alpha and E2, PG 6-ketoF1 alpha and thromboxane (TX) B2 , as well as inactive metabolites of prostacyclin (PGI2 ) and TXA2 were detected. F decreased the production of vasodilator PRs PGI2 and PGE2 in MVB. Mo prevented these alterations and increased PGE2 in controls. Vasoconstrict or PRs PGF2 alpha and TXA2 release was not modified. (6) Mo treatment, beyond its known lowering effect on glycemia, prevents the reduction in the vascular release of vasodilator PR observed in this model. This could be one of the mechanisms by which Mo avoids the increase in BP caused by F overload in the rat.

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“…The cell culture chambers were also constantly perfused with preincubation medium applied with a flow driven by gas pressure system at 4 psi for 30 min establishing basal levels of secretion. Each group of islets were then treated with 350 μL of different concentrations of T3s (α, γ, and δ; Cayman Chemical, USA) treatment mediums that were perfused at 4 psi for 1 h. The final concentrations of T3s in the treatment mediums (D-PBS with 10% v/v FBS) were 150, 300, 600, and 1200 μM in glucose alone (2.8 and 16.7 mM, as basal and stimulant, respectively; Wicksteed et al, 2003; Ronnebaum et al, 2006; Monfared and Pournourmohammadi, 2010) or together with 30 mM KCl (Sato and Henquin, 1998; Yajima et al, 1999; Shackman et al, 2004). The treated groups were all compared with their respective positive control group (1 μM of glyburide; Kinard and Satin, 1995; Wicksteed et al, 2003) and negative control group (glucose and, or KCl medium only).…”

Section: Methodsmentioning

confidence: 99%

Effects of Tocotrienols on Insulin Secretion-Associated Genes Expression of Rat Pancreatic Islets in a Dynamic Culture

et al. 2016

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Tocotrienols (T3) are well-known for their antioxidant properties besides showing therapeutic potential in clinical complications such as hyperlipidemia induced by diabetes. The aim of this study was to determine the effects of δ-T3, γ-T3, and α-T3 on insulin secretion-associated genes expression of rat pancreatic islets in a dynamic culture. Pancreatic islets freshly isolated from male Wistar rats were treated with T3 for 1 h at 37°C in a microfluidic system with continuous operation. The cells were collected for total RNA extraction and reverse-transcribed, followed by measurement of insulin secretion-associated genes expression using quantitative real-time polymerase chain reaction. Molecular docking experiments were performed to gain insights on how the T3 bind to the receptors. Short-term exposure of δ- and γ-T3 to pancreatic β cells in a stimulant glucose condition (16.7 mM) significantly regulated preproinsulin mRNA levels and insulin gene transcription. In contrast, α-T3 possessed less ability in the activation of insulin synthesis level. Essentially, potassium chloride (KCl), a β cell membrane depolarising agent added into the treatment further enhanced the insulin production. δ- and γ-T3 revealed significantly higher quantitative expression in most of the insulin secretion-associated genes groups containing 16.7 mM glucose alone and 16.7 mM glucose with 30 mM KCl ranging from 600 to 1200 μM (p < 0.05). The findings suggest the potential of δ-T3 in regulating insulin synthesis and glucose-stimulated insulin secretion through triggering pathway especially in the presence of KCl.

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Teucrium polium Complex with Molybdate Enhance Cultured Islets Secretory Function

Cited by 14 publications

References 25 publications

Teucrium polium extracts stimulate GLUT4 translocation to the plasma membrane in L6 muscle cells

Teucrium polium extracts stimulate GLUT4 translocation to the plasma membrane in L6 muscle cells

Sodium molybdate prevents hypertension and vascular prostanoid imbalance in fructose‐overloaded rats

Effects of Tocotrienols on Insulin Secretion-Associated Genes Expression of Rat Pancreatic Islets in a Dynamic Culture

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