Tetrazolium reduction assays under-report cell death provoked by clinically relevant concentrations of proteasome inhibitors

Harris, Michael A.; Hawkins, Christine J.; Miles, Mark A.

doi:10.1007/s11033-020-05530-3

Cited by 1 publication

(1 citation statement)

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“…The use of cell viability for peptides' molecular validation has limitations because cell viability is very diverse due to the redox potential of the cell population, cell membrane integrity, or the activity of cellular enzymes that form a snapshot of cytotoxicity or drug efficacy [86]. Even specially designed cell viability indicators, such as fluorescence microscopes, microplate readers or flow cytometers, have positive and negative attributes with their sensitivity, reliability and compatibility being determined by relevant cell lines [87]. Williams et al, 2017 [84] employed site-directed mutagenesis to generate variant HDPs from previously identified ones and used molecular validation techniques, such as a lateral flow device (LFD) binding assay and nanotechnologies recombinant technologies, to explore their activities against HIV p24.…”

Section: Molecular Validation Techniques Used For Hdpsmentioning

confidence: 99%

Biomedical Relevance of Novel Anticancer Peptides in the Sensitive Treatment of Cancer

Bakare

Gokul

et al. 2021

Biomolecules

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The global increase in cancer mortality and economic losses necessitates the cautious quest for therapeutic agents with compensatory advantages over conventional therapies. Anticancer peptides (ACPs) are a subset of host defense peptides, also known as antimicrobial peptides, which have emerged as therapeutic and diagnostic candidates due to several compensatory advantages over the non-specificity of the current treatment regimens. This review aimed to highlight the ravaging incidence of cancer, the use of ACPs in cancer treatment with their mechanisms, ACP discovery and delivery methods, and the limitations for their use. This would create awareness for identifying more ACPs with better specificity, accuracy and sensitivity towards the disease. It would also promote their efficacious utilization in biotechnology, medical sciences and molecular biology to ease the severity of the disease and enable the patients living with these conditions to develop an accommodating lifestyle.

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Section: Molecular Validation Techniques Used For Hdpsmentioning

confidence: 99%