Tetraploid Black Locust (<i>Robinia Pseudoacacia</i>L.) Increased Salt Tolerance by Activation of the Antioxidant System

Meng, Fanjuan; Pang, Hongying; Huang, Fenglang; Liu, Lei; Wang, Yanjie

doi:10.5504/bbeq.2012.0110

Cited by 14 publications

(15 citation statements)

References 51 publications

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“…However, some studied showed that there is relationship between the ploidy level of plants and the number of chloroplast in stomatal guard cells in Acacia mearnsii 14 and Brassica oleracea 15 . In addition, our previous study showed that salt stress resulted in distorted chloroplasts, swollen thylakoid membranes, accumulation of plastoglobules, and increased starch grains in diploid black locust compared to those in tetraploid black locust 16 . Therefore, there is direct or indirect relationship between nuclear genome ploidy and chloroplast function.…”

Section: Discussionmentioning

confidence: 93%

Physiological and proteomic responses to salt stress in chloroplasts of diploid and tetraploid black locust (Robinia pseudoacacia L.)

Meng

Luo

Wang

et al. 2016

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Salinity is an important abiotic stressor that negatively affects plant growth. In this study, we investigated the physiological and molecular mechanisms underlying moderate and high salt tolerance in diploid (2×) and tetraploid (4×) Robinia pseudoacacia L. Our results showed greater H2O2 accumulation and higher levels of important antioxidative enzymes and non-enzymatic antioxidants in 4× plants compared with 2× plants under salt stress. In addition, 4× leaves maintained a relatively intact structure compared to 2× leaves under a corresponding condition. NaCl treatment didn’t significantly affect the photosynthetic rate, stomatal conductance or leaf intercellular CO2 concentrations in 4× leaves. Moreover, proteins from control and salt treated 2× and 4× leaf chloroplast samples were extracted and separated by two-dimensional gel electrophoresis. A total of 61 spots in 2× (24) and 4× (27) leaves exhibited reproducible and significant changes under salt stress. In addition, 10 proteins overlapped between 2× and 4× plants under salt stress. These identified proteins were grouped into the following 7 functional categories: photosynthetic Calvin-Benson Cycle (26), photosynthetic electron transfer (7), regulation/defense (5), chaperone (3), energy and metabolism (12), redox homeostasis (1) and unknown function (8). This study provides important information of use in the improvement of salt tolerance in plants.

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Section: Discussionmentioning

confidence: 93%

Physiological and proteomic responses to salt stress in chloroplasts of diploid and tetraploid black locust (Robinia pseudoacacia L.)

Meng

Luo

Wang

et al. 2016

Sci Rep

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“…Several studies have examined the relationship between polyploidy and enhanced ecological tolerance through altered nutrient uptake and performance under diverse soil conditions (te Beest et al, 2012). Increased salinity tolerance in polyploids has been found in a wide range of species (e.g., Meng et al, 2011, 2012; Yan et al, 2015). Polyploid Solidago gigantea also exhibited greater tolerance to certain calcium treatments (Schlaepfer et al, 2010).…”

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Enhanced rhizobial symbiotic capacity in an allopolyploid species ofGlycine(Leguminosae)

Powell

Doyle

2016

American J of Botany

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The allopolyploid is characterized by transgressive responses to rhizobia for some variables, while also combining certain parental diploid responses such that its capacity for interactions with rhizobia appears to be greater than for either diploid progenitor. This overall enhanced nodulation capacity and the ability to make greater gains from exposure to both rhizobia and additional nitrogen indicate a greater potential of the allopolyploid to benefit from these factors both generally and in a context of colonization.

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“…TRP is both a promising and preferred species for timber and reforestation because of its rapid growth rate (Meng et al 2012;Wang et al 2013) and because it can be used as a source of nectar and as a soil improvement species (i.e., nitrogen-fixing) (Li and Jiang 2006). Moreover, it can also be used as a fine feed for domestic fowl and livestock because its fleshy leaves contain various nutrients (Meng et al 2012). Importantly, TRP is extremely adaptable to adverse environments such as salt, drought, cold, pests, and nutrient deficiency (Wang et al 2011.…”

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confidence: 99%

Comparative proteomic analysis of tetraploid black locust (Robinia pseudoacacia L.) cuttings in different phases of adventitious root development

Sheng

Zhao

Zhang

et al. 2014

Trees

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Key message Comparative proteome analysis revealed the proteins that are differentially expressed during the processes of tetraploid black locust (Robinia pseudoacacia L.) adventitious root formation, which are involved in carbohydrate binding, energy and metabolism, and stress response. Abstract The tetraploid black locust (Robinia pseudoacacia L.) is an important fast-growing tree used in afforestation and is also a protein-rich feed for poultry. Currently, the primary approach for tetraploid black locust reproduction is through cutting propagation. To identify the specific proteins in the rooting process, including the formation of adventitious root primordium (ARP) and the elongation of adventitious root (EAR), a comparative proteomic analysis of the ARP and EAR of tetraploid black locust was performed to identify related proteins that may be involved in regulating ARP formation and the process of EAR. High-resolution two-dimensional electrophoresis (2-DE) followed by colloidal Coomassie staining and mass spectrometric (MS) analysis was used to identify differentially expressed proteins in ARP and EAR. A total of 84 protein spots showed significant expression changes by 2-DE and were successfully identified by MALDI TOF/ TOF MS/MS. Of these, 29 proteins were differentially expressed during the ARP phase, and the remaining 55 proteins did so in the EAR phase. The majority of identified proteins were classified into functional categories, including carbohydrate binding, energy and metabolism, protein degradation/folding and import, cellular cytoskeleton, hormonerelated and oxidation reactions, stress response, cell proliferation, and transcription regulation. Quantitative real-time PCR analysis was performed for selected proteins and showed that not all of the protein expression levels were consistent with the mRNA levels. This study provides the basis for further functional studies of differentially expressed proteins, which will contribute to the understanding of the biochemical processes in adventitious root primordium formation and the elongation of adventitious root. Abbreviations 2-DE Two-dimensional gel electrophoresis MALDI Matrix assisted laser desorption ionization TOF Time of flight TOF MS Time of flight mass spectrometry MS Mass spectrometry IntroductionTetraploid black locust (Robinia pseudoacacia L.) (TRP), which can be produced by doubling the chromosomes of a diploid black locust (2n 9 2) artificially, is a leguminous Communicated by R. Hampp. Electronic supplementary materialThe online version of this article (

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Tetraploid Black Locust (Robinia PseudoacaciaL.) Increased Salt Tolerance by Activation of the Antioxidant System

Cited by 14 publications

References 51 publications

Physiological and proteomic responses to salt stress in chloroplasts of diploid and tetraploid black locust (Robinia pseudoacacia L.)

Physiological and proteomic responses to salt stress in chloroplasts of diploid and tetraploid black locust (Robinia pseudoacacia L.)

Enhanced rhizobial symbiotic capacity in an allopolyploid species ofGlycine(Leguminosae)

Comparative proteomic analysis of tetraploid black locust (Robinia pseudoacacia L.) cuttings in different phases of adventitious root development

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