Tetrahymena Pot2 Is a Developmentally Regulated Paralog of Pot1 That Localizes to Chromosome Breakage Sites but Not to Telomeres

Cranert, Stacey; Heyse, Serena R.; Linger, Benjamin R.; Lescasse, Rachel; Price, Carolyn M.

doi:10.1128/ec.00204-14

Cited by 12 publications

(8 citation statements)

References 53 publications

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“…Pot2p is the first factor shown to associate specifically with Cbs regions in vivo, at the time of chromosome breakage (Cranert et al, 2014). Pot2p is a paralog of Pot1p, which is required for telomere maintenance.…”

Section: Resultsmentioning

confidence: 99%

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Structure of the germline genome of Tetrahymena thermophila and relationship to the massively rearranged somatic genome

Hamilton

Kapusta

Huvos

et al. 2016

eLife

139

264

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The germline genome of the binucleated ciliate Tetrahymena thermophila undergoes programmed chromosome breakage and massive DNA elimination to generate the somatic genome. Here, we present a complete sequence assembly of the germline genome and analyze multiple features of its structure and its relationship to the somatic genome, shedding light on the mechanisms of genome rearrangement as well as the evolutionary history of this remarkable germline/soma differentiation. Our results strengthen the notion that a complex, dynamic, and ongoing interplay between mobile DNA elements and the host genome have shaped Tetrahymena chromosome structure, locally and globally. Non-standard outcomes of rearrangement events, including the generation of short-lived somatic chromosomes and excision of DNA interrupting protein-coding regions, may represent novel forms of developmental gene regulation. We also compare Tetrahymena’s germline/soma differentiation to that of other characterized ciliates, illustrating the wide diversity of adaptations that have occurred within this phylum.DOI: http://dx.doi.org/10.7554/eLife.19090.001

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Section: Resultsmentioning

confidence: 99%

“…It is notable that a homolog of a telomerase subunit, Pot2, was recently found to associate with chromosome breakage sites in T. thermophila (Cranert et al, 2014). It may take part in the telomerase-dependent healing of the newly broken ends.…”

Section: Cbs Duplication Clades: Three Case Historiesmentioning

confidence: 99%

Structure of the germline genome of Tetrahymena thermophila and relationship to the massively rearranged somatic genome

Hamilton

Kapusta

Huvos

et al. 2016

eLife

139

264

View full text Add to dashboard Cite

show abstract

“…The Tetrahymena TtPOT1-encoded protein is essential for telomere length maintenance and prevents checkpoint activation much like the vertebrate POT1 proteins (110). However, TtPOT2 protein does not associate with chromosome ends, but instead localizes to internal sites in macronuclear chromosomes that are destined for developmentally programmed cleavage and de novo telomere formation (111). In E. crassus, the telomere endbinding protein caps chromosome ends (109).…”

Section: Gene Duplication: Refining the Landscape Of Telomere Proteinmentioning

confidence: 99%

Back to the future: The intimate and evolving connection between telomere-related factors and genotoxic stress

Barcenilla

Shippen

2019

Journal of Biological Chemistry

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Edited by Patrick Sung The conversion of circular genomes to linear chromosomes during molecular evolution required the invention of telomeres. This entailed the acquisition of factors necessary to fulfill two new requirements: the need to fully replicate terminal DNA sequences and the ability to distinguish chromosome ends from damaged DNA. Here we consider the multifaceted functions of factors recruited to perpetuate and stabilize telomeres. We discuss recent theories for how telomere factors evolved from existing cellular machineries and examine their engagement in nontelomeric functions such as DNA repair, replication, and transcriptional regulation. We highlight the remarkable versatility of protection of telomeres 1 (POT1) proteins that was fueled by gene duplication and divergence events that occurred independently across several eukaryotic lineages. Finally, we consider the relationship between oxidative stress and telomeres and the enigmatic role of telomere-associated proteins in mitochondria. These findings point to an evolving and intimate connection between telomeres and cellular physiology and the strong drive to maintain chromosome integrity.

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“…Cbs напоминает сайты-мишени для транспозаз на концах транспозонов [13]. Интересно, что консенсус Cbs содержит в своем составе последовательность C 2 A 2 C 2 , похожую на перестроенный теломерный повтор t. thermophila C 4 A 2 [26], а белок Pot2, специфически взаимодействующий с Cbs при фрагментации хромосом [27], относится к тому же семейству, что и белок Pot1, участвующий в поддержании теломер. Являются ли эти совпадения случайными или отражают эволюцию Cbs, пока остается неясным, но предполагают, что сами Cbs и пока неизвестный белок, узнающий Cbs и инициирующий разрыв, могли произойти из мобильного элемента, доместицированного в геноме именно для обеспечения фрагментации хромосом при формировании генома МАК [13].…”

Section: особенности формирования генома макронуклеуса у Tetrahymena unclassified

RNA interference in formation of the somatic genome of ciliates Paramecium and Tetrahymena

Nekrasova

Потехин

2018

Ecological genetics

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Ciliates are the model of choice to study RNA interference, the mechanism playing key role in biology of these protists. The genome scanning processes of two ciliates, Tetrahymena and Paramecium (Oligohymenophorea), leading to formation of the somatic genome from the chromosomes of the generative nucleus are compared in the review. Matching of several simulta neously present in one cell genomes is mediated by small RNAs and results in precise reproduction of maternal somatic genome in the sexual progeny.

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Tetrahymena Pot2 Is a Developmentally Regulated Paralog of Pot1 That Localizes to Chromosome Breakage Sites but Not to Telomeres

Cited by 12 publications

References 53 publications

Structure of the germline genome of Tetrahymena thermophila and relationship to the massively rearranged somatic genome

Structure of the germline genome of Tetrahymena thermophila and relationship to the massively rearranged somatic genome

Back to the future: The intimate and evolving connection between telomere-related factors and genotoxic stress

RNA interference in formation of the somatic genome of ciliates Paramecium and Tetrahymena

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