Tetrahydroxystilbene Glucoside Exerts Cytoprotective Effect against Hydrogen Peroxide-induced Cell Death Involving ROS Production and Antioxidant Enzyme Activation

Abstract

The impact of gastric diseases on human health, such as chronic gastritis, duodenal, gastric ulceration and adenocarcinoma, remains a big issue deserving worldwide attention. Oxidative stress is the major gastric pathologic feature and plays an essential role in the multiple progressions of gastric diseases [1] . The excessive generation of reactive oxygen species (ROS) is associated with cell death and generation of ROS is associated with cell death an keep cellular homeostasis, excessive ROS can cause damage to lipids and proteins as well as single stranded DNA breaks [2] and gastric epithelium is exposed to higher ROS lever than other tissues [3,4] . Therefore, it is necessary for cells to effectively counteract ROS generation by triggering their own defensive mechanisms with the help of antioxidants. Hemeoxygenase-1 (HO-1) is a highly inducible, stress-responsive protein (also called heat shock protein 32), which catalyzes the first and ratelimiting step in heme degradation, a potent oxidant [5] . Indeed, ample evidence currently supports the notion that HO-1 serves to provide potent cytoprotective effects in many in vitro and in vivo models of oxidantinduced cellular and tissue injury. The NADPHquinone oxidoreductase 1 (NQO1) is a predominantly cytosolic enzyme which provides cells with multiple layers of protection against oxidative stress, including the direct detoxification of highly reactive quinones [6,7] . *Address for correspondence E-mail: zhj@zcmu.edu.cnThis is an open access article distributed under terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

Biological Effects of Tetrahydroxystilbene Glucoside: An Active Component of a Rhizome Extracted from Polygonum multiflorum

“…NADPH-quinone oxidoreductase 1 (NQO1) is a predominantly cytosolic enzyme that provides cells with multiple layers of protection against oxidative stress, including the direct detoxification of highly reactive quinones [ 29 ]. The pretreatment of cells with TSG (5 and 10 μ M) for 24 h can attenuate H 2 O 2 -induced ROS production and upregulate the expression of antioxidant enzymes HO-1 and NQO1 in a dose-dependent manner [ 30 ]. These results suggest that TSG is a potent antioxidant.…”