Tetrahedral framework nucleic acids regulate osteogenic differentiation potential of osteoporotic adipose-derived stem cells

Effects of tension on mitochondrial autophagy and osteogenic differentiation of periodontal ligament stem cells

“…The regulation of intracellular mitochondrial content is a potential mechanism by which autophagy regulates the early targeted differentiation of stem cells, and mitochondrial autophagy may be a potential therapeutic target in bone-related diseases. [32][33][34] Therefore, this study focused on exploring the changes in the osteogenic differentiation ability of PDLSCs under CTS. The autophagy process was affected by the addition of the autophagy chloroquine and the autophagy inducer rapamycin, and the changes in mitochondrial apoptosis in PDLSCs after applying CTS and the relationship between these changes and osteogenic differentiation were discussed.…”

“…Additionally, rapamycin promotes the mitochondrial autophagy pathway, thus promoting the osteogenic differentiation of stem cells. The regulation of intracellular mitochondrial content is a potential mechanism by which autophagy regulates the early targeted differentiation of stem cells, and mitochondrial autophagy may be a potential therapeutic target in bone‐related diseases 32–34 …”

Advanced glycation end products inhibit the osteogenic differentiation potential of adipose-derived stem cells in mice through autophagy

“…Among them, adipose-derived stem cells (ASCs) are easy to obtain in large quantities and have a good multidirectional differentiation potential, such as osteogenic, adipogenic, and angiogenic, and have become a hotspot in bone regeneration research [11][12][13][14].…”

Tailorable 3DP Flexible Scaffolds with Porosification of Filaments Facilitate Cell Ingrowth and Biomineralized Deposition

“…11,12 In addition, the microstructure could affect specific protein adsorption to regulate stem cell differentiation. 13,14 With the development of high-precision fabrication technology toward medicine, 3D printing technology provides a new means for fabricating tissue engineering scaffolds with adjustable pores and mechanics. 15−17 Poly(lactic-co-glycolic acid) (PLGA) with good biocompatibility, adjustable biodegradability, and mechanical properties had been widely used as 3D printing bone scaffold inks.…”

“…The design of bone repair materials inspired by the compositions and structures of healthy bone had proven to be an ideal approach to benefit bone defect regeneration. , Natural cancellous bone consists of interconnected bone trabecula networks with a large number of pores, which provides sufficient mechanical support and helps to maintain the biological functions of bone tissue . Various bone grafts with porous structures that mimic the natural cancellous bone have been made and have shown great advances in bone defect repair. , Studies had shown that the porous structures endowed bone scaffolds multiple advantages. , For example, larger sized pores (200∼350 μm) provided space for bone ingrowth and angiogenesis, , interconnected pores facilitated cell immigration and nutrient transport, and smaller sized pores (<20 μm) increased the specific surface area of the scaffold to promote the absorption of endogenous bone-inducing biomolecules and other biological factors, which acted as reservoirs for these bioactive factors. , In addition, the microstructure could affect specific protein adsorption to regulate stem cell differentiation. , …”