Tetracycline residues in honey after hive treatment

Martel, Anne‐Claire; Zeggane, Sarah; Drajnudel, Patrick; Faucon, Jean-Paul; Aubert, Michel

doi:10.1080/02652030500469048

Cited by 87 publications

(60 citation statements)

References 6 publications

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“…HPLC analysis condition was fixed (as/at) : Flow 0.7 ml/ dk, Mobil phase (mobile phase A: 1% acetonitrile, mobile phase 2: 80% acetonitrile), temperature of colon and reactor: 45 ºC, injected sample: 70 μ, and time 36 min; FLOURESAN detector condition was fixed as : excitation wavelength 400, and Emission wavelength 495 nm (Martel et al, 2006;Münstedt, ThortenRademacher & Petz, 2005;Reybroeck, 2003).…”

Section: Investigation Of Different Botanical Origin Of Honey Types Wmentioning

confidence: 99%

“…The antibiotic analysis diagnosed in the honey samples was by Charm II honey Assay (Martel, Zeggane, Drajnudel, Faucon, & Aubert, 2006;Münstedt, ThortenRademacher & Petz, 2005;Reybroeck, 2003). Standard solutions (10 ppb) of Sulfamethazine, Tetracycline, and Streptomycin were used as a reference to determine the residue limit point in the honey samples.…”

Section: Antibiotic Analysismentioning

confidence: 99%

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Investigation of Different Botanical Origin of Honey Types with Respect to Food Safety

2017

American Research Journal of Agriculture

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Abstract:In this research, antibiotic (sulfamethazine, tetracycline, streptomycin), pesticides (amitraz, coumaphos, fluvalinate) and naphthalene and phosphine residues in 210 honey samples collected from 7 different geographic regions of Turkey were investigated. Accordingly, in the investigations conducted during 2007, out of 210 analyzed honey samples, 92 samples tested positive for antibiotic, 19 samples tested positive for pesticides, 7 samples tested positive for naphthalene and 11 samples tested positive for phosphine residue. Also, antibiotic, pesticides and naphthalene residues were not found in 92 samples out of 210. The obtained results from the study confirmed the routine use of antibiotics and pesticides by beekeepers for the control of certain honey bee diseases and parasites. The results show that some beekeepers in Turkey used antibiotics and pesticides whereas some beekeepers did not use these veterinary drugs. Generally, the risk of residue remaining in the honey in not high and could be reduced with the use of licensed for honey bee veterinary drugs, the correct use of these veterinary drugs and correct application of these prescribed treatment against honey bee disease.

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Section: Investigation Of Different Botanical Origin Of Honey Types Wmentioning

confidence: 99%

Section: Antibiotic Analysismentioning

confidence: 99%

Investigation of Different Botanical Origin of Honey Types with Respect to Food Safety

2017

American Research Journal of Agriculture

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“…For example, tetracycline has been used to inhibit Paenibacillus larvae growth to limit American foulbrood (AFB) and Melissococcus plutonius growth to limit European foulbrood (EFB) (Martel et al, 2006). For microsporidia (Nosema) infections, treatment with fumagillin B has been found to be very effective, including in the control of infections by treating the hive box with fumagillin B (Higes et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Pathogen detection and gut bacteria identification in Apis cerana indica in Thailand

Nonthapa¹,

Chanchao²

2015

Afr. J. Biotechnol.

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Pathogen infection of honeybees can lead to economic losses in apiculture. The earlier the pathogen contamination can be found the better it will likely be for the treatment of the infected colony and prevention of the spread of the pathogen within and between colonies. A total of 50 colonies of Apis cerana were sampled in Samut-Songkhram (five colonies) and Chumphon (45 colonies) provinces in the central and the south of Thailand, respectively. Diagnostic multiplex polymerase chain reaction (PCR) revealed that 20, 6, 4, 20 and 0% of the samples were infected by Paenibacillus larvae, Nosema ceranae, Nosema apis, Ascosphaera apis and Sacbrood virus (Morator aetatulus), respectively. Positive amplified PCR products of target genes were sequenced. A phylogenetic tree was constructed by the neighbor-joining (NJ) distance based, using the Phylogenetic Analysis Using Parsimony (PAUP 4.0b10) program. The phylogenetic relationship of each pathogen, based on the partial sequence of the 16S rRNA of P. larvae, N. ceranae and N. apis, and of the 5.8S rRNA of A. apis, revealed a significant difference from the non-Thai isolates of these pathogens, but no significant geographical isolation between the different Thai apiaries, although it separated some into closely related clusters. In order to reduce the use of antibiotics in an apiary, bacteria in the gut of healthy bees were focused. Interestingly, Bifidobacterium species, Lactobacillus species, Bacillus species, Lactobacillus spp. and other lactic acid bacteria, were isolated from larvae and adult workers, but gave conflicting preliminary identities based on their biochemistry-morphology versus sequence analysis of a partial fragment (1.4 kb) of their 16S rRNA.

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“…Kurittu et al [11] used microbiological inhibition test technique to measure TET residues, but the test lacked specificity and sensitivity. Therefore, a lot of traditional analytical methods have been developed for the replacement of microbiological assays, such as high performance liquid chromatography (HPLC) [12][13][14], capillary electrophoresis (CE) [15,16], fluorescence (FL) [17,18] and mass spectrometric detection (MS) [19,20]. These traditional analytical methods are highly sensitive and specific, but they are not suitable for widespread and rapid detection.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Aptasensor Based on Prussian Blue-Chitosan-Glutaraldehyde for the Sensitive Determination of Tetracycline

et al. 2014

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Abstract:In this paper, a novel and sensitive electrochemical aptasensor for detecting tetracycline (TET) with prussian blue (PB) as the label-free signal was fabricated. A PB-chitosan-glutaraldehyde (PB-CS-GA) system acting as the signal indicator was developed to improve the sensitivity of the electrochemical aptasensor. Firstly, the PB-CS-GA was fixed onto the glass carbon electrode surface. Then, colloidal gold nanoparticles (AuNPs) were droped onto the electrode to immobilize the anti-TET aptamer for preparation of the aptasensor. The stepwise assembly process of the aptasensor was characterized by cyclic voltammetry (C-V) and scanning electron microscope (SEM). The target TET captured onto the electrode induced the current response of the electrode due to the non-conducting biomoleculars. Under the optimum operating conditions, the response of differential pulse voltammetry (DPV) was used for detecting the concentration of TET. The proposed aptasensor showed a high sensitivity and a wide linear range of 10 −9 ∼ 10 −5 M and 10 −5 ∼ 10 −2 M with the correlation coefficients of 0.994 and 0.992, respectively. The detection limit was 3.2×10 −10 M (RSD 4.12%). Due to its rapidity, sensitivity and low cost, the proposed aptasensor could be used as a pre-scanning method in TET determination for the analysis of livestock products.

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Tetracycline residues in honey after hive treatment

Cited by 87 publications

References 6 publications

Investigation of Different Botanical Origin of Honey Types with Respect to Food Safety

Investigation of Different Botanical Origin of Honey Types with Respect to Food Safety

Pathogen detection and gut bacteria identification in Apis cerana indica in Thailand

Electrochemical Aptasensor Based on Prussian Blue-Chitosan-Glutaraldehyde for the Sensitive Determination of Tetracycline

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