Tetracycline-regulated expression of OLIG2 gene in human dental pulp stem cells lead to mouse sciatic nerve regeneration upon transplantation

Askari, Nahid; Yaghoobi, Mohammad; Shamsara, Mehdi; Esmaeili‐Mahani, Saeed

doi:10.1016/j.neuroscience.2015.07.088

Cited by 22 publications

(17 citation statements)

References 37 publications

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“…They exhibit all the characteristics of MSCs that make them appropriate options for clinical application, being capable of following multi-lineage differentiation, (including neural differentiation [202]) when under suitable culture conditions; presenting self-renewal capacity [203]; expressing MSC phenotypic markers [204], stemness-related markers, cytoskeleton-related markers [205]; and, as expected, not expressing hematopoietic markers (Figure 4b) [203]. Specifically, DPSCs express neural markers [206,207]; produce neurotrophic factors; stimulate the differentiation, growth, and orientation of growing axons; and differentiate into active and functional neurons [208,209]. Compared to other types of MSCs, DPSCs have higher clonogenic capacity, high proliferation, and a larger stem/progenitor cell population.…”

Section: Dental Pulp Mesenchymal Stem Cellsmentioning

confidence: 86%

“…Knowing the importance of oligodendrocyte lineage transcription factor 2 in the oligodendrogenic pathway, Askari et al were capable to induce the differentiation of DPSCs into oligodendrocytes by transfection of a tetracycline (Tet)-inducible system expressing oligodendrocyte lineage transcription factor 2 gene. These differentiated cells were then used in the treatment of a local demyelinating lesion of the mouse sciatic nerve by lysolecithin, with observation of repair and regeneration of the injured nerve [209]. The comparative use of DPSCs and neuronal cells originating from the differentiation of DPSCs in the treatment of a 5 mm lesion in the rat sciatic nerve proved that both cell types promoted functional and muscle contraction improvements, associated with the identification of specific markers for angiogenesis, even though no specific differences between the two cell types in promoting nerve regeneration were identified [219].…”

Section: Dental Pulp Mesenchymal Stem Cellsmentioning

confidence: 99%

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Biomaterials and Cellular Systems at the Forefront of Peripheral Nerve Regeneration

Alvites¹,

Branquinho²,

Caseiro³

et al. 2020

Peripheral Nerve Disorders and Treatment

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Peripheral nerve injuries remain a common clinical complication, and currently available therapies present significant limitations, often resulting in poor and suboptimal outcomes. Despite significant developments in microsurgical approaches in the last decades, no effective treatment options have been disclosed. Current research focuses on the optimization of such microsurgical techniques and on their combination with other pro-regenerative factors, such as mesenchymal stem cells or biomaterials. Mesenchymal stem cells present a remarkable capacity for bioactive molecule production that modulates inflammatory and regenerative processes, stimulating peripheral nerve regeneration. In parallel, efforts have been directed towards the development of biomaterial nerve guidance channels and nerve conduits. These biomaterials have been optimized in terms of biodegradability, ability to release bioactive factors, incorporation of cellular agents, and internal matrix architecture (to enable cellular migration and mimic native tissue morphology and to generate and bear specific electrical activity). The current literature review presents relevant advances in the development of mesenchymal stem cell and biomaterial-based therapeutic approaches aiming at the peripheral nerve tissue regeneration in diverse lesion scenarios, also exploring the advances achieved by our research group in this field in recent years.

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Section: Dental Pulp Mesenchymal Stem Cellsmentioning

confidence: 86%

Section: Dental Pulp Mesenchymal Stem Cellsmentioning

confidence: 99%

Biomaterials and Cellular Systems at the Forefront of Peripheral Nerve Regeneration

Alvites¹,

Branquinho²,

Caseiro³

et al. 2020

Peripheral Nerve Disorders and Treatment

View full text Add to dashboard Cite

show abstract

“…When isolated from patients with neurofibromatosis type 1, DPSCs have a proliferation rate higher than that of normal cells; thus, DPSCs represent a suitable model for neurofibromatosis type 1 [ 72 ]. Moreover, DPSC-derived oligoprogenitor cells showed high therapeutic potential in an animal model of sciatic nerve injury [ 73 ], indicating its potential as a therapeutic for amelioration of myelin injuries in the PNS [ 74 ]. When SHED-CM was investigated for peripheral nerve regeneration, SHED-CM-treated Schwann cells exhibited a significantly increased number of neuronal and angiogenesis related genes.…”

Section: Dscs and Neurodegenerative Disordersmentioning

confidence: 99%

Mechanisms underlying dental-derived stem cell-mediated neurorestoration in neurodegenerative disorders

et al. 2018

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BackgroundNeurodegenerative disorders have a complex pathology and are characterized by a progressive loss of neuronal architecture in the brain or spinal cord. Neuroprotective agents have demonstrated promising results at the preclinical stage, but this has not been confirmed at the clinical stage. Thus far, no neuroprotective drug that can prevent neuronal degeneration in patients with neurodegenerative disorders is available.Main bodyRecent studies have focused on neurorestorative measures, such as cell-based therapy, rather than neuroprotective treatment. The utility of cell-based approaches for the treatment of neurodegenerative disorders has been explored extensively, and the results have been somewhat promising with regard to reversing the outcome. Because of their neural crest origin, ease of harvest, accessibility, ethical suitability, and potential to differentiate into the neurogenic lineage, dental-derived stem cells (DSCs) have become an attractive source for cell-based neurorestoration therapies. In the present review, we summarize the possible use of DSC-based neurorestoration therapy as an alternative treatment for neurodegenerative disorders, with a particular emphasis on the mechanism underlying recovery in neurodegenerative disorders.ConclusionTransplantation research in neurodegenerative diseases should aim to understand the mechanism providing benefits both at the molecular and functional level. Due to their ease of accessibility, plasticity, and ethical suitability, DSCs hold promise to overcome the existing challenges in the field of neurodegeneration through multiple mechanisms, such as cell replacement, bystander effect, vasculogenesis, synaptogenesis, immunomodulation, and by inhibiting apoptosis.

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“…The conditioned medium of SHED containing the factors involved in multiple neuro-regenerative mechanisms might account for the results. Iran scientists also showed that improvement of behavioral symptoms was efficiently observed in the mouse model of local sciatic demyelination damage by lysolecithin after transplantation of the DPSCs, to which a tetracycline (Tet)-inducible system expressing OLIG2 gene had been transfected (Askari et al, 2015). And Maraldi et al confirmed strong potential of bioengineered constructs of stem cell (including dental pulp derived stem cell)-collagen scaffold for correcting large cranial defects in a rat model and highlighting the role of stem cells in neovascularization during skeletal defect reconstruction (Maraldi et al, 2013).…”

Section: Application Of Oral Derived Stem Cells In Non-oral Diseasesmentioning

confidence: 99%

Application of Stem Cells in Oral Disease Therapy: Progresses and Perspectives

Yang¹,

Qiu²,

Zhou³

et al. 2017

Front. Physiol.

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Stem cells are undifferentiated and pluripotent cells that can differentiate into specialized cells with a more specific function. Stem cell therapies become preferred methods for the treatment of multiple diseases. Oral and maxillofacial defect is one kind of the diseases that could be most possibly cured by stem cell therapies. Here we discussed oral diseases, oral adult stem cells, iPS cells, and the progresses/challenges/perspectives of application of stem cells for oral disease treatment.

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Tetracycline-regulated expression of OLIG2 gene in human dental pulp stem cells lead to mouse sciatic nerve regeneration upon transplantation

Cited by 22 publications

References 37 publications

Biomaterials and Cellular Systems at the Forefront of Peripheral Nerve Regeneration

Biomaterials and Cellular Systems at the Forefront of Peripheral Nerve Regeneration

Mechanisms underlying dental-derived stem cell-mediated neurorestoration in neurodegenerative disorders

Application of Stem Cells in Oral Disease Therapy: Progresses and Perspectives

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