Tetracycline inhibits the nitric oxide synthase activity induced by endotoxin in cultured murine macrophages

D’Agostino, Pietro; Arcoleo, Francesco; Barbera, C.; Bella, G. Di; Rosa, Massimo La; Misiano, Gabriella; Milano, Salvatore; Brai, M.; Cammarata, Giuseppe; Feo, Salvatore; Cillari, Enrìco

doi:10.1016/s0014-2999(98)00046-6

Cited by 40 publications

(18 citation statements)

References 31 publications

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“…The effects of tetracyclines include the inhibition of matrix metalloproteases [15,16]. It has recently been shown that tetracyclines inhibit the nitric oxide synthase activity induced by endotoxin in cultured murine macrophages [17,18] and human OA-affected cartilage [19,20]. However, the effects of tetracyclines on the inducible NO synthase of OA synovial cells are unknown.…”

Section: Introductionmentioning

confidence: 99%

Tetracyclines inhibit nitrosothiol production by cytokine-stimulated osteoarthritic synovial cells

et al. 2001

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Section: Introductionmentioning

confidence: 99%

Tetracyclines inhibit nitrosothiol production by cytokine-stimulated osteoarthritic synovial cells

et al. 2001

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“…It may also inhibit the activity of matrix metalloproteinases (MMPs),6 – 8 the synthesis of these proteases,9 10 collagen,11 interleukin-1 (IL-1),12 13 nitrous oxide (NO) in lipopolysaccharide (LPS) activated macrophage14 15 and activated B cell function 16. Potentially these properties all relate to the ability of doxycycline to chelate metal ions4 and contribute to its therapeutic benefits.…”

mentioning

confidence: 99%

Does orally administered doxycycline reach the tear film?

Smith

Khan-Lim

Cook

et al. 2008

British Journal of Ophthalmology

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“…However, anti-inflammatory properties of tetracyclines have been described. D'Agostino et al reported a suppression of nitric oxide synthase by tetracycline in macrophages, thereby inhibiting the generation of oxygen radicals [49]. Furthermore, tetracyclines have been shown to have beneficial immunomodulatory properties in several inflammatory conditions, such as multiples sclerosis, Parkinson's disease and rheumatoid arthritis [50].…”

Section: Discussionmentioning

confidence: 99%

Large-Scale Integrative Analysis of Epigenetic Modifications Induced by Isotretinoin, Doxycycline and Metronidazole in Murine Colonic Intestinal Epithelial Cells

et al. 2017

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Environmental factors are playing a central role in triggering inflammatory responses in the intestine. There is increasing evidence that the development of inflammatory bowel disease (IBD) is deriving from an aberrant immune response to the commensal gut microbiota triggered by various environmental factors in a susceptible host. A vitamin A derivate used in acne therapy (isotretinoin) has been inconsistently associated with the onset of IBD. However, what needs to be considered is the previous treatment of acne patients with antibiotics that are also associated with the development of IBD, thus representing a crucial confounding factor. Here, we studied whether doxycycline (acne therapy), metronidazole (IBD therapy) or isotretinoin are able to induce alterations in DNA methylation and microRNA expression patterns in murine colonic intestinal epithelial cells (IECs). Additionally, we analyzed time-dependent changes in the aforementioned epigenetic mechanisms to study how epigenetic signatures evolve over time. As for changes in DNA methylation, we found isotretinoin to have strong demethylating effects, while antibiotic treatment had only a moderate impact. Isotretinoin-mediated demethylation resolved after a washout phase, not supporting an association between isotretinoin treatment and IBD. Regarding microRNA and mRNA expression, isotretinoin and doxycycline, but not metronidazole, potentially induce long-term changes in microRNA/mRNA expression profiles towards the down-regulation of immune responses. Analysis of time-dependent DNA methylation showed stable marks over a time frame of 4 weeks. Furthermore, novel microRNAs were identified (e.g., microRNA-877-3p), which might be of relevance in IEC development.

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Tetracycline inhibits the nitric oxide synthase activity induced by endotoxin in cultured murine macrophages

Cited by 40 publications

References 31 publications

Tetracyclines inhibit nitrosothiol production by cytokine-stimulated osteoarthritic synovial cells

Tetracyclines inhibit nitrosothiol production by cytokine-stimulated osteoarthritic synovial cells

Does orally administered doxycycline reach the tear film?

Large-Scale Integrative Analysis of Epigenetic Modifications Induced by Isotretinoin, Doxycycline and Metronidazole in Murine Colonic Intestinal Epithelial Cells

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