Tethered capsule endomicroscopy enables less invasive imaging of gastrointestinal tract microstructure

Gora, Michalina J.; Sauk, Jenny; Carruth, Robert W.; Gallagher, Kevin A.; Suter, Melissa J.; Nishioka, Norman S.; Kava, Lauren; Rosenberg, Mireille; Bouma, Brett E.; Tearney, Guillermo J.

doi:10.1038/nm.3052

Cited by 263 publications

(227 citation statements)

References 13 publications

Supporting

Mentioning

224

Contrasting

Unclassified

Order By: Relevance

“…57 and 58 demonstrated 19.6 µm and 15.7 µm transverse resolutions, respectively), and an SNR of 29 dB from a 3-µm diameter microsphere illuminated by a pulse energy of 500-nJ in a 9.2 µm beam diameter. As shown in the in vivo and ex vivo image demonstrations, the major benefit of the OR-PAEM over existing IVM techniques [44][45][46][47][48][49][50][51][52][53][54][55][56] lies in its label-free angiographic imaging capability, which provides critical image in experimental biology and clinical medicine. Although other groups [57,58] developed endoscopic devices with optical focusing and achieved an even smaller probe diameter (i.e., ~1.1 mm in the case of ref.…”

Section: Discussionmentioning

confidence: 99%

“…OR-PAM realizes the key benefits of PAT in biological experimentation through its high-resolution imaging capability; further, its unique optical absorption-based contrast mechanism enables it to complement conventional highresolution microscopy tools, such as confocal microscopy [44][45][46][47][48][49], two-photon microscopy [44][45][46][50][51][52][53], and optical coherence tomography [44][45][46][53][54][55][56]. So far, considerable efforts have been made to improve the spatial resolution [15], scanning speed [7,12], and functional imaging capability [4][5][6][7][8][9] of OR-PAM.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Label-free optical-resolution photoacoustic endomicroscopy in vivo

Yang

Chen

et al. 2015

SPIE Proceedings

View full text Add to dashboard Cite

Intravital microscopy techniques have become increasingly important in biomedical research because they can provide unique microscopic views of various biological or disease developmental processes in situ. Here we present an opticalresolution photoacoustic endomicroscopy (OR-PAEM) system that visualizes internal organs with a much finer resolution than conventional acoustic-resolution photoacoustic endoscopy systems. By combining gradient index (GRIN) lens-based optical focusing and ultrasonic ring transducer-based acoustic focusing, we achieved a transverse resolution as fine as ~10 μm at an optical working distance of 6.5 mm. The OR-PAEM system's high-resolution intravital imaging capability is demonstrated through animal experiments.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Label-free optical-resolution photoacoustic endomicroscopy in vivo

Yang

Chen

et al. 2015

SPIE Proceedings

View full text Add to dashboard Cite

show abstract

“…Tethered capsule OCT (also referred to as tethered capsule endomicroscopy, TCE) was reported by Gora et al 73 in 2013. The capsule (which measures 24.8 mm by 12.8 mm) is swallowed by the patient, rather than being introduced by endoscope, and provides 3D imaging via a pull-back manoeuvre (FIG.…”

Section: Microbiota Changesmentioning

confidence: 99%

“…4). This technology is considerably less invasive than endoscopy, as the capsule relies on natural peristalsis to pass through the gastrointestinal tract rather than being externally manoeuvred, and can be undertaken in unsedated (adult) patients 73 . TCE has been applied to the assessment of Barrett oesophagus, with volumetric images of the entire oesophagus collected in as little as 60 s (REF.…”

Section: Microbiota Changesmentioning

confidence: 99%

The potential role of optical biopsy in the study and diagnosis of environmental enteric dysfunction

Thompson

Hughes

Anastasova

et al. 2017

Nat Rev Gastroenterol Hepatol

View full text Add to dashboard Cite

| Environmental enteric dysfunction (EED) is a disease of the small intestine affecting children and adults in low and middle income countries. Arising as a consequence of repeated infections, gut inflammation results in impaired intestinal absorptive and barrier function, leading to poor nutrient uptake and ultimately to stunting and other developmental limitations. Progress towards new biomarkers and interventions for EED is hampered by the practical and ethical difficulties of cross-validation with the gold standard of biopsy and histology. Optical biopsy techniques -which can provide minimally invasive or noninvasive alternatives to biopsy -could offer other routes to validation and could potentially be used as point-of-care tests among the general population. This Consensus Statement identifies and reviews the most promising candidate optical biopsy technologies for applications in EED, critically assesses them against criteria identified for successful deployment in developing world settings, and proposes further lines of enquiry. Importantly, many of the techniques discussed could also be adapted to monitor the impaired intestinal barrier in other settings such as IBD, autoimmune enteropathies, coeliac disease, graft-versus-host disease, small intestinal transplantation or critical care. Members of the board and experts met to discuss the horizon scan in London, UK, in November 2015 and San Francisco, USA, in February 2016. Literature searches were performed using Web of Science, as well as through the advice of the advisory board and experts. The results of the horizon scan are presented in this Consensus Statement, which entails a review of EED, a discussion of technologies deemed relevant to EED (based on literature searches and the opinions of the advisory board and experts), and a series of recommendations for the future application of the rele vant technologies to the study and diagnosis of EED. In the Recommendations section, each technology has been assessed according to its suitability for application to EED and this assessment includes an evaluation of the cost, invasiveness and appropriateness for developing countries of each device and/or technique. This assessment was performed in a qualitative manner based on currently available information (for example, the present cost and size of the devices) and the opinions of the authors as to feasible future develop ment. Technique suitability for use in developing countries was determined in a qualitative manner after consideration of the need for advanced infrastructure and high levels of training for either system use or data analysis. Probable deployment time scales have also been assigned to each technique based on the current states of development and the degree of valid ation work that will be required before application to EED. These timescales have been tentatively defined as short-term (suitable for deployment immediately or within ~1 year), mediumterm (suit able for deployment within ~1-3 years) and long-term (suitable for deployme...

show abstract

“…Ainsi l'optical frequency domain imaging (OFDI) est une méthode dérivée de la tomographie par cohérence optique (OCT) qui permet, en temps réel, d'obtenir des images de la microstructure d'un tissu à la fois en surface et en profondeur. Incorporée dans une capsule ingérable par le patient ( Figure 1A), elle a récemment été utilisée par une équipe du Massachusetts general hospital (Boston) chez 24 patients [3,4]. L'examen durait en moyenne cinq minutes.…”

Section: L'endobrachyoesophage : Un Modèle De Pathologie Précancéreuseunclassified