Tests for the viability of seeds

Freeland, P. W.

doi:10.1080/00219266.1976.9654060

Cited by 18 publications

(9 citation statements)

References 4 publications

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“…To check whether there existed a seed bank containing viable seeds, soil samples (depth 20 cm) were taken with a corer (9.51 cm^, total area sampled 76.1 cm^). The viability of seeds caught by the traps or sampled from snow or soil, was determined with the tetrazoliumtest (Freeland 1976).…”

Section: Methodsmentioning

confidence: 99%

Relationship between the seed rain and the establishment of vegetation in two areas abandoned after peat harvesting

Salonen

1987

Ecography

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In this study the number and species composition of diaspores dispersing into two newly abandoned peat harvesting areas in Finland were investigated. In an area abandoned six years earlier a total of 2978 living seeds m−2, representing 18 species, was captured by using water‐filled traps during two summers. In a one‐year‐old succession area the total number of living seeds m−2 was 2241, representing 16 plant species. The soil seed banks were found to be empty of viable seeds. In neither area there appeared to be any relation between number of dispersing seeds and of plant individuals of the same species in the pioneer vegetation. It is suggested that unfavourable conditions on the soil surface for seed germination and seedling growth of most dispersed species are majors factor in determining the structure of the established vegetation.

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Section: Methodsmentioning

confidence: 99%

Relationship between the seed rain and the establishment of vegetation in two areas abandoned after peat harvesting

Salonen

1987

Ecography

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“…Daisy 'seeds' are properly termed 'fruits', but the term 'seed' is used for simplicity. Initial seed viability was assessed on 8 replicates of 25 seeds of each species using tetrazolium (Freeland 1976). The small seeds of C. apiculatum and L squamatus stained poorly, so viability of these species was assessed visually.…”

Section: Seed Burialmentioning

confidence: 99%

Seed Longevity of Six Native Forbs in a Closed Themeda triandra Grassland

Lunt¹

1995

Aust. J. Bot.

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Seeds of six native forbs̵2Arthropodium strictum R.Br., Burchardia umbellata R.Br., Bulbine bulbosa (R.Br.) Haw., Chrysocephalum apiculatum (Labill.) Steetz, Craspedia variabilis Everett & Doust and Leptorhynchos squamatus (Labill.) Less.-were sown on and below the soil surface in a closed, native grassland dominated by Themeda triandra Forsskal. Replicate seed lots were recovered after 2, 4, 6, 9 and 12 months, and viability was assessed. Less than 7% of sown surface seeds of B. bulbosa, B. umbellata, C. variabilis and L. squamatus, and less than 10% of buried seeds of A. strictum, B. umbellata and C. variabilis remained viable after 12 months. Virtually all losses of Liliaceae seeds were due to germination. Fates of Asteraceae seeds were difficult to assess accurately after 6 months, but germination accounted for most seed losses. Burial significantly promoted longevity of B. bulbosa, C. variabilis and L. squamatus seeds. No obvious relationship existed between seed longevity and taxonomic group (Liliaceae versus Asteraceae) or seed mass, for surface or buried seeds; the response of the large-seeded lily, B. bulbosa, was most similar to that of the small-seeded daisy, L. squamatus. Of the six species, C. apiculatum appears to have the greatest potential to accumulate a soil seed bank beneath a closed grass canopy, owing to its small seed size, inhibition of germination beneath a closed canopy, both on and below the soil surface, and sustained viability of buried seeds. Naturally dispersed seeds of the other five species are likely to form smaller, transient or short-term seed banks.

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“…Within each microhabitat, the six lots of 25 fruits were placed in a row at 6 cm intervals, with the endpoints marked by stakes. Of the remaining 100 fruits, two additional lots of 25 were tested for viability in the lab at this time, using the tetrazolium test (Freeland 1976). A final two lots of 25 fruits were stored dry at 4°C in the dark in a constant cold chamber at the University of Canterbury.…”

Section: Methodsmentioning

confidence: 99%

A buried seed experiment using caryopses ofChionochloa macraZotov (Danthonieae: Poaceae), South Island, New Zealand

Spence

1990

New Zealand Journal of Botany

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A buried seed experiment was conducted in the alpine zone of the Craigieburn Range, South Island, New Zealand, with caryopses ofthe common large tussock grass Chionochloa macra Zotov. Overwinter survival of caryopses enclosed in the palea and lemma and buried 1 cm below the soil or litter surface averaged 78% of those initially viable. Summer mortality was high, with only 5.5% of ungerminated fruits still viable by autumn. The major losses from the seed bank of this species were, in order of importance, germination, summerembryo mortality, and winter embryo mortality. There were significantdifferences in fruit survival, germination, and success of seedling establishment in the two most important microhabitats for seedlings, Poa colensoi turf, and litter/bryophyte mat.

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Tests for the viability of seeds

Cited by 18 publications

References 4 publications

Relationship between the seed rain and the establishment of vegetation in two areas abandoned after peat harvesting

Relationship between the seed rain and the establishment of vegetation in two areas abandoned after peat harvesting

Seed Longevity of Six Native Forbs in a Closed Themeda triandra Grassland

A buried seed experiment using caryopses ofChionochloa macraZotov (Danthonieae: Poaceae), South Island, New Zealand

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