Testosterone Secretion by Foetal Rat Testes in Vitro

Picon, R.

doi:10.1677/joe.0.0710231

Cited by 116 publications

(55 citation statements)

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“…These findings are similar to those observed in other species such as rats [6,14,20], sheep [21] and pigs [16]. In rats, the onset of testicular testosterone production has been reported to start on Day 15.5 p.c.…”

supporting

confidence: 89%

Immunolocalization of Steroidogenic Enzymes in the Fetal, Neonatal and Adult Testis of the Shiba Goat

et al. 2005

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supporting

confidence: 89%

Immunolocalization of Steroidogenic Enzymes in the Fetal, Neonatal and Adult Testis of the Shiba Goat

et al. 2005

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“…These results suggest that GnRH-R mRNA is translated into a functional receptor protein very soon after its formation, but this cannot be verified by immunodetection, as antibodies against GnRH-R are not available at the present time. It is well documented that, in vivo, after reaching its maximum around 18·5 dpc, testosterone production by the testis remains unchanged (Warren et al 1984), or decreases when it is expressed per cell (Picon 1976, Tapanainen et al 1984, Saez 1994. During the same period, the density of cells producing testosterone per testis has been reported to be constant (Tapanainen et al 1984, Kerr & Knell 1988, Saez 1994.…”

Section: Discussionmentioning

confidence: 98%

Fetal expression of GnRH and GnRH receptor genes in rat testis and ovary

Botte

Chamagne²,

Carre³

et al. 1998

Journal of Endocrinology

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The identification of gonadal gonadotropin-releasing hormone receptors (GnRH-R) and evidence of direct inhibitory effects of GnRH agonists upon steroidogenesis in adult rat gonads, lend credence to a putative intragonadal role of a locally secreted GnRH or GnRH-like peptide. Using reverse transcription-polymerase chain reaction followed by Southern blot hybridization and sequencing, we identified, both in the ovary and in the testis of fetal and adult rats, a fully processed GnRH messenger RNA (mRNA), the sequence of which, in adult testis, was identical to that found in the hypothalamus. We also detected in the testis, but not in the ovary, a transcript containing the first intron.The ontogeny of GnRH and GnRH-R gene expression was studied in rat gonads from 14·5 to 21·5 days postcoitum (dpc), using dot blot hybridization of total RNA. During this period, the levels of cyclophilin mRNA normalized to total RNA remained unchanged. Thus, we used cyclophilin as an internal standard. GnRH mRNA was detected in the ovary at 18·5 dpc, four days later than in the testis, and similar levels were found in both sexes at birth. GnRH-R mRNA was present at 14·5 dpc in the testis and at 15·5 dpc in the ovary, with the levels at 21·5 dpc being 2·4 times higher in the testis than in the ovary. GnRH and GnRH-R mRNA levels increased in both sexes in late fetal development, but this increase appeared two days sooner in the ovary compared with the testis, thus supporting the hypothesis that expression of the GnRH and GnRH-R genes is regulated in a sexdependent manner during fetal development. In all cases, expression of GnRH and GnRH-R preceded gonadotropin receptors in the gonads and initiation of gonadotropin secretion by the pituitary.

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“…However, functional gonadal steroids can be detected in the blood stream of the rat in as early as the last week of gestation [27,28] In the learning process, there are two components: short-term memory for recent events -working memory [29] and long-term acquired memory for past eventsreference memory [30].…”

Section: Introductionmentioning

confidence: 99%

Prenatal testosterone improves the spatial learning and memory by protein synthesis in different lobes of the brain in the male and female rat

et al. 2008

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Abstract:The high density of the steroid hormone receptors in the structures of temporal lobe involved in learning and memory, such as the hippocampus, perirhinal cortex, entorhinal cortex and amigdaloid complex, shows that there must be a direct relationship between gonadal hormones and organizational effects of steroid hormones in those structures during development of the nervous system. The present study was undertaken in order to investigate the effect of testosterone administration during the third week of gestation on the spatial memory formation of the offspring rats and the level of soluble proteins in the temporal lobe and frontal lobe of brain, as evidence of important organizational effects of androgens during prenatal development in brain sexual dimorphism. Animals have received testosterone undecanoate on days 14, 15, 16 and 19, 20, 21 of gestation. Learning and memory tests were started 100 days after the testosterone treatment. At the end of the experiments, the temporal and frontal lobes of brain were removed for assessing the level of soluble proteins. Testosterone treatment significantly improved spontaneous alternations percentage of male offspring in Y-maze task comparative with female offspring and reference memory in radial 8 arm-maze task (decreasing in number of reference memory errors in both male and female offspring groups), suggesting effects of both short and long-term memory. Also, testosterone significantly increased the brain soluble protein level of treated female rats in 14-16 prenatal days compared with the control group as well as the brain soluble protein level of treated male rats. These results suggest that steroid hormones play an important role in the spatial learning and memory formation by means of protein synthesis in different lobes of the brain.

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Testosterone Secretion by Foetal Rat Testes in Vitro

Cited by 116 publications

References 0 publications

Immunolocalization of Steroidogenic Enzymes in the Fetal, Neonatal and Adult Testis of the Shiba Goat

Immunolocalization of Steroidogenic Enzymes in the Fetal, Neonatal and Adult Testis of the Shiba Goat

Fetal expression of GnRH and GnRH receptor genes in rat testis and ovary

Prenatal testosterone improves the spatial learning and memory by protein synthesis in different lobes of the brain in the male and female rat

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