Testosterone replacement therapy and cardiovascular disease

Auerbach, Jeremy M.; Khera, Mohit

doi:10.1038/s41443-021-00516-6

Cited by 4 publications

(2 citation statements)

References 91 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Testosterone has multiple physiological functions. However, recent studies show that the expression level of testosterone is highly associated with the occurrence, development, and prognosis of various diseases, including cardiovascular disease, depression, obesity, and prostate cancer [ 8 – 10 ]. For example, Kumar and Banu et al pointed out that estradiol and testosterone could promote the growth and metastasis of PTC [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Testosterone promotes the migration, invasion and EMT process of papillary thyroid carcinoma by up-regulating Tnnt1

Jiang

et al. 2023

J Endocrinol Invest

View full text Add to dashboard Cite

Purpose To explore the key genes and molecular pathways in the progression of thyroid papillary carcinoma (PTC) promoted by testosterone using RNA-sequencing technology, and to provide new drug targets for improving the therapeutic effect of PTC. Methods Orchiectomy (ORX) was carried out to construct ORX mouse models. TPC-1 cells were subcutaneously injected for PTC formation in mice, and the tumor tissues were collected for RNA-seq. The key genes were screened by bioinformatics technology. Tnnt1 expression in PTC cells was knocked down or overexpressed by transfection. Cell counting kit-8 (CCK-8), colony formation assay, scratch assay and transwell assay were adopted, respectively, for the detection of cell proliferation, colony formation, migration and invasion. Besides, quantification real-time polymerase chain reaction (qRT-PCR) and western blot were utilized to determine the mRNA and protein expression levels of genes in tissues or cells. Results Both estradiol and testosterone promoted the growth of PTC xenografts. The key gene Tnnt1 was screened and obtained by bioinformatics technology. Functional analysis revealed that overexpression of Tnnt1 could markedly promote the proliferation, colony formation, migration, invasion, and epithelial-to-mesenchymal transition (EMT) process of PTC cells, as well as could activate p38/JNK pathway. In addition, si-Tnt1 was able to inhibit the cancer-promoting effect of testosterone. Conclusion Based on the outcomes of bioinformatics and basic experiments, it is found that testosterone can promote malignant behaviors such as growth, migration, invasion and EMT process of PTC by up-regulating Tnnt1 expression. In addition, the function of testosterone may be achieved by activating p38/JNK signaling pathway.

show abstract

Section: Introductionmentioning

confidence: 99%

Testosterone promotes the migration, invasion and EMT process of papillary thyroid carcinoma by up-regulating Tnnt1

Jiang

et al. 2023

J Endocrinol Invest

View full text Add to dashboard Cite

show abstract

“…Besides, testosterone is a widely prescribed drug. Several studies have found that testosterone plays a crucial role in male gonadal dysfunction, cardiovascular disease, and type 2 diabetes [5][6][7]. Research has also demonstrated that testosterone can result in abnormal proliferation of prostatic epithelial and stromal tissues [8,9].…”

Section: Introductionmentioning

confidence: 99%

Testosterone promotes human foreskin fibroblast growth through miR-143-3p targeting IGFBP-3

2023

Journal of Men's Health

View full text Add to dashboard Cite

Testosterone is an important male hormone, which could improve the maintenance and recovery of gonadal function in males as well as the repair of human hypospadias and cell fibrosis. Our study focused on investigating the regulatory effect of testosterone in human foreskin fibroblasts (HFF-1) and regulatory mechanisms involved. In this study, HFF-1 cells were treated with testosterone, and cell viability and migration were assessed by cell counting kit-8 (CCK8) and Transwell assays. The expression levels of androgen receptor (AR), miR-143-3p and insulin-like growth factor binding protein-3 (IGFBP-3) were measured by quantitative real-time PCR (qRT-PCR), Western blotting, and immunofluorescence. In addition, a potential binding site for miR-143-3p on IGFBP-3 was predicted and its direct binding was further confirmed by a dual luciferase reporter assay. These results showed that testosterone increased the viability and migration of HFF-1 cells. Testosterone could down-regulate miR-143-3p and up-regulate IGFBP-3 and AR. Overexpression of miR-143-3p hindered HFF-1 cell viability and negatively regulated IGFBP-3, whereas inhibition of IGFBP-3 impeded cell viability and migration. Furthermore, miR-143-3p was found to directly bind to IGFBP-3. Overexpression of IGFBP-3 countered the regulation of HFF-1 cells by miR-143-3p mimics. In conclusion, this study showed that testosterone promoted the proliferation and migration of HFF-1 cells and AR signaling, at least via the miR-143-3p/IGFBP-3 axis. This discovery presents a novel insight for testosterone application in male disorders like hypospadias.

show abstract

Aromatase Inhibitors May Increase the Risk of Cardiometabolic Complications in Adolescent Boys

Besci,

Akçura,

Acinikli

et al. 2023

Pediatr Cardiol

View full text Add to dashboard Cite

Testosterone replacement therapy and cardiovascular disease

Cited by 4 publications

References 91 publications

Testosterone promotes the migration, invasion and EMT process of papillary thyroid carcinoma by up-regulating Tnnt1

Testosterone promotes the migration, invasion and EMT process of papillary thyroid carcinoma by up-regulating Tnnt1

Testosterone promotes human foreskin fibroblast growth through miR-143-3p targeting IGFBP-3

Aromatase Inhibitors May Increase the Risk of Cardiometabolic Complications in Adolescent Boys

Contact Info

Product

Resources

About