Testosterone identifies hatchling sex for Mojave desert tortoises (Gopherus agassizii)
M. A. Walden,
Kevin J. Loope,
Elizabeth A. Hunter
et al.
Abstract:The threatened Mojave desert tortoise (Gopherus agassizii) exhibits temperature-dependent sex determination, and individuals appear externally sexually monomorphic until sexual maturity. A non-surgical sex identification method that is suitable for a single in situ encounter with hatchlings is essential for minimizing handling of wild animals. We tested (1) whether plasma testosterone quantified by enzyme-linked immunosorbent assay differentiated males from females in 0–3 month old captive hatchlings, and (2) … Show more
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