Testosterone content and variability in adult and young Romanov sheep

Abstract

In the article, the authors conducted a comprehensive study of the interior, including biochemical, physiological, physicochemical, cytogenetic, and other indicators in sheep of the Romanov breed of Western Siberia. For the study, blood serum samples were taken from 10 rams-producers and 30 lambs at four months. Conventional methods took blood samples. Analyses were performed in the biochemistry laboratory of Novosibirsk State Agrarian University using the technique of competitive enzyme immunoassay with the reagent kit “SteroidIFAtestosterone” for the quantitative determination of testosterone concentration in blood serum. In the sheep breeding area, the authors constantly monitored the content of heavy metals in water, soil, feed, organs, and tissues. The concentration of chemical elements did not exceed the permissible exposure limit. Enzyme-linked immunosorbent assay was performed on a Thermo Scientific Multiskan FC analyzer. The authors performed statistical processing of experimental data using standard methods of descriptive statistics, the statistical programming language, or the RStudio data analysis environment (using the functions DescrStats, Summary, Sd, Read. Table, Write. Table, Aes). The authors established the influence of the age of ontogenesis on the testosterone content in Romanov rams. Presented figure with two variation curves with high transgression. The average testosterone level was 1.8 times higher in lambs (0.774 mmol/l) than in lambs. Testosterone concentration was characterized by high phenotypic variability. The authors have identified reference intervals for testosterone in blood serum depending on environmental conditions in sheep of the Romanov breed of Western Siberia.