Testosterone concentration in the seminal plasma of cocks

Abstract

Testosterone concentrations in the seminal plasma of cocks ranged from 0.46 to 5.05 ng/ml and were substantially lower than in blood plasma. No significant variation was noted in seminal plasma testosterone concentrations during the light phase of the day, whereas the concentration in blood declined over this period. Spermatozoal concentration and seminal testosterone decreased in the third sample of the semen collected sequentially at 3 h intervals. Testosterone concentrations in seminal plasma (1.57 +/- 0.17 ng/ml) and in the semen from the ductus deferens (1.34 +/- 0.24 ng/ml) were not significantly different.

Genetic differences in yolk testosterone levels influence maternal hormone deposition in the second laying cycle in Japanese quails

“…Testosterone concentrations were measured in both yolk extracts (20 μL aliquot) and plasma samples (50 μL aliquot) by radioimmunoassay using [1,2,6,7-3 H]-T (Amersham Biosciences, UK, specific activity 3.52 TBq/mmol) and a specific antibody generated in rabbits against testosterone-3-(carboxy-methyl) oxime bovine serum albumin conjugate. The cross-reactivity of the antiserum was 9.6% with 5α-dihydrotestosterone, 0.1% with androstenediol and lower than 0.1% with other steroids (Zeman et al, 1986). Yolk and plasma samples were run separately within 4 assays with a mean intra-assay variation coefficient of 4% and inter-assay variation coefficient of 5.2%.…”

Matrilineal inheritance of a key mediator of prenatal maternal effects

“…) and a specific antibody generated in rabbits against testosterone-3-(carboxy-methyl) oxime bovine serum albumin conjugate [25]. The sensitivity of the assay was 1.62 + 0.17 pg per tube.…”

Preliminary results on intersexual differences in gene expression of chemokine K203 in mononuclear cells of chicken

“…Testosterone was measured using [1,2,6, H(N)]-testosterone (Perkin Elmer, USA, specific activity 63.47 Ci/mmoL) and the specific antibody generated in rabbits against testosterone 3-(O-carboxymethyl)oxime bovine serum albumin (BSA) conjugate (Zeman et al, 1986). Bound and free fractions were separated with dextrancoated charcoal and centrifuged with 3000 g at +4 °C for 10 min.…”

“…Hormone concentrations were measured by radioimmunoassay (Zeman et al, 1986) without extraction in males and after extraction with a mixture of diethyl ether/petroleum ether in females (Okuliarova et al, 2011). Testosterone was measured using [1,2,6, H(N)]-testosterone (Perkin Elmer, USA, specific activity 63.47 Ci/mmoL) and the specific antibody generated in rabbits against testosterone 3-(O-carboxymethyl)oxime bovine serum albumin (BSA) conjugate (Zeman et al, 1986).…”