Testosterone Assays: Guidelines for the Provision of a Clinical Biochemistry Service

Ismail, A. A. A.; Astley, Peter; Cawood, Marion L.; Short, Frances; Wakelin, Kate; Wheeler, Michael J.

doi:10.1177/000456328602300202

Cited by 19 publications

(5 citation statements)

References 22 publications

(3 reference statements)

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“…11 This lack of specificityis thought to relate to the use in the direct methods of antisera raised against testosterone conjugated to protein via C-19and highlightsyet another area of immunoassay methodology i.e, antisera specificity, which can affect assay validity.…”

Section: Reference Methodsmentioning

confidence: 99%

Quality Assurance of Hormone Analyses

Burrin

1988

Ann Clin Biochem

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Section: Reference Methodsmentioning

confidence: 99%

Quality Assurance of Hormone Analyses

Burrin

1988

Ann Clin Biochem

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“…Laboratory analysis for plasma glucose were all carried out through the glucose oxidase enzymatic method with same brands of laboratory reagents purchased from Randox Laboratory manufacturers, United Kingdom, while serum TT was analyzed via competitive enzyme immunoassay method with reagents sourced from Monoblind Incorporated, United States. 12,13 To maintain and monitor analytical precision and accuracy, three levels of commercial control sera, sourced from the same reagent manufacturers, were interspersed in each run of the laboratory analysis.…”

Section: Exclusion Criteriamentioning

confidence: 99%

Relationship between glucose homeostatic disorders and testosterone in adult Nigerian women with polycystic ovary syndrome

Amadi

Green

2018

Int J Reprod Contracept Obstet Gynecol

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Background: Polycystic ovary syndrome (PCOS) is associated with hyperandrogenemia which is hypothesized to induce glucose homeostastic disorders among women with the syndrome. This study was thereby set to investigate the status of glucose homeostatic disorders and their relationship with testosterone among women with PCOS in Port Harcourt, Nigeria.Methods: This was a retrospective study of 224 women with PCOS who had visited a tertiary hospital in Nigeria over a 10-year period. The laboratory characteristics of these patients were evaluated. Records on age, fasting plasma glucose (FPG) and serum total testosterone (TT) were collected and analyzed using Shapiro-Wilk, descriptive, chi-square, Fisher’s exact, two-way analysis of variance, and Pearson’s tests. A p-value <0.05 was considered significant.Results: Glucose homeostatic disorders were documented in 49.1% of the study population. Impaired fasting glucose (IFG) and diabetes mellitus (DM) was observed among 37.5% and 11.6% of the study population respectively. There was difference in the mean TT levels among those with normal FPG, IFG, and DM (P<0.001). Those with DM had higher TT levels (2.47 ± 0.83 nmol/l) than those with IFG (2.12 ± 0.63 nmol/l) and normal glucose homeostatic status (1.63 ± 0.47 nmol/l). TT correlated positively with FPG among the overall study population (r=0.465; p-value = < 0.001), among those with IFG (r = 0.591; p-value=0.004), and among those with DM (r=0.404; p-value =0.012).Conclusions: Glucose homeostatic disorders are common among PCOS patients in our environment, especially among those with higher testosterone levels. This warrants screening of all PCOS patients for glucose disorders as part of their management strategies.

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“…The assays were performed on the RANDOX (Randox Laboratories Limited, UK) using standard kit reference methods. Total testosterone, LH & FSH were estimated on the Advia Centaur'CP immunoassay system by chemiluminescence method [6,7] . TSH was quantitated by immunoradiometric assay by IRMA kit (IRMAK -9) [8] .…”

Section: Routine Biochemical Investigationsmentioning

confidence: 99%

Comparison of Dehydroepiandrosterone Sulfate to Free Testosterone and Luteinizing Hormone to Follicular Stimulating Hormone Ratios in Polycystic Ovarian Syndrome Women

Pahwa¹,

Bala²,

Gupta³

et al. 2017

JGNB

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Objective: To compare Dehydroepiandrosterone Sulfate to Free Testosterone (DHEAS/FT) and Luteinizing Hormone to Follicular Stimulating Hormone (LH/FSH) ratios in Polycystic Ovarian Syndrome (PCOS) women. Material and Methods: The present study was done in the Department of Biochemistry in collaboration with Department of Obstetrics and Gynaecology in which 30 PCOS cases and 30 ages matched healthy controls were enrolled. Fasting venous blood samples were collected from cases and controls for routine biochemical and hormone analysis after obtaining written consent & complete history. Results: We found that free testosterone of cases (10.3 ± 17.1 pg/dL) showed significantly higher values than controls (1.84 ± 1.4 pg/dL) with p = 0.011 and had significantly positive correlation with Body Mass Index (BMI) with p = 0.014. DHEAS and FSH of cases had no significant raised value than controls (p > 0.05). LH of cases (12.7 ± 8.9 mIU/mL) had significantly higher than controls (7.02 ± 6.2 mIU/mL) with p = 0.006. DHEAS/FT of cases (0.815 ± 0.95) showed significantly lower than controls (1.922 ± 1.53) with p = 0.001. LH/ FSH ratio (2.25 ± 2.3) of cases was significantly higher than controls (1.01 ± 0.72) with p = 0.008. LH/FSH ratio had 30% sensitivity and 93% specificity, but DHEAS/FT ratio had 16.7% sensitivity and 100% specificity for PCOS. Conclusion: In conclusion, DHEAS/FT ratio can be considered as a specific marker than LH/FSH ratio and LH/FSH ratio as a better sensitive marker than DHEAS/FT ratio in PCOS women.

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Testosterone Assays: Guidelines for the Provision of a Clinical Biochemistry Service

Cited by 19 publications

References 22 publications

Quality Assurance of Hormone Analyses

Quality Assurance of Hormone Analyses

Relationship between glucose homeostatic disorders and testosterone in adult Nigerian women with polycystic ovary syndrome

Comparison of Dehydroepiandrosterone Sulfate to Free Testosterone and Luteinizing Hormone to Follicular Stimulating Hormone Ratios in Polycystic Ovarian Syndrome Women

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