Testosterone and FSH modulate Sertoli cell extracellular secretion: Proteomic analysis

Mancuso, Francesca; Calvitti, Mario; Milardi, Domenico; Grande, Giuseppe; Falabella, G.; Arato, Iva; Giovagnoli, Stefano; Vincenzoni, Federica; Mancini, Francesca; Nastruzzi, Claudio; Bodo, Maria; Baroni, Tiziano; Castagnola, Massimo; Marana, Riccardo; Pontecorvi, Alfredo; Calafiore, Riccardo; Luca, Giovanni

doi:10.1016/j.mce.2018.04.001

Cited by 26 publications

(18 citation statements)

References 45 publications

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“…At stage VII-VIII of the epithelial cycle, the preleptotene and leptotene spermatocytes must move through the BTB and enter the adluminal compartment 10 . Most researchers focused on the synchronization of spermatogenesis currently, but few of them have addressed the issue from the perspective of Sertoli cells [11][12][13][14][15][16][17] .…”

Section: Introductionmentioning

confidence: 99%

Multiple signaling pathways in Sertoli cells: recent findings in spermatogenesis

2019

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The functions of Sertoli cells in spermatogenesis have attracted much more attention recently. Normal spermatogenesis depends on Sertoli cells, mainly due to their influence on nutrient supply, maintenance of cell junctions, and support for germ cells’ mitosis and meiosis. Accumulating evidence in the past decade has highlighted the dominant functions of the MAPK, AMPK, and TGF-β/Smad signaling pathways during spermatogenesis. Among these pathways, the MAPK signaling pathway regulates dynamics of tight junctions and adherens junctions, proliferation and meiosis of germ cells, proliferation and lactate production of Sertoli cells; the AMPK and the TGF-β/Smad signaling pathways both affect dynamics of tight junctions and adherens junctions, as well as the proliferation of Sertoli cells. The AMPK signaling pathway also regulates lactate supply. These signaling pathways combine to form a complex regulatory network for spermatogenesis. In testicular tumors or infertile patients, the activities of these signaling pathways in Sertoli cells are abnormal. Clarifying the mechanisms of signaling pathways in Sertoli cells on spermatogenesis provides new insights into the physiological functions of Sertoli cells in male reproduction, and also serves as a pre-requisite to identify potential therapeutic targets in abnormal spermatogenesis including testicular tumor and male infertility.

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Section: Introductionmentioning

confidence: 99%

Multiple signaling pathways in Sertoli cells: recent findings in spermatogenesis

2019

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“…In vitro studies also confirm this aspect. Indeed, highly purified pre-pubertal Sertoli porcine, incubated for 48 h with FSH or FSH and testosterone, showed the presence of Sertolian extracellular vesicles containing specific mRNAs [ 20 ]. In detail, the proteomic analysis identified 29 proteins related to receptor binding activity.…”

Section: Discussionmentioning

confidence: 99%

Retrospective Monocentric Clinical Study on Male Infertility: Comparison between Two Different Therapeutic Schemes Using Follicle-Stimulating Hormone

Condorelli

Cannarella

Crafa

et al. 2021

JCM

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Follicle-stimulating hormone (FSH) is a therapeutic option in patients with idiopathic oligozoospermia and normal FSH serum levels. However, few studies have evaluated which dose of FSH is more effective. The aim of this study was to compare the clinical efficacy of the two most frequently used FSH treatment regimens: 75 IU daily vs. 150 IU three times a week. Patients were retrospectively assigned to two groups. The first group (n = 24) was prescribed highly purified FSH (hpFSH) 75 IU/daily (Group A), and the second group (n = 24) was prescribed hpFSH 150 IU three times a week (Group B) for three months. Before and after treatment, each patient underwent semen analysis, evaluation of the percentage of DNA-fragmented spermatozoa, assessment of testicular volume (by ultrasonography), and measurement of FSH and total testosterone (TT) serum levels. Treatment with hpFSH significantly improved conventional sperm parameters. In detail, sperm concentration increased significantly after treatment only in Group A, whereas total sperm count, percentage of spermatozoa with progressive motility, normal morphology, or alive improved significantly in both groups. Interestingly, the percentage of sperm DNA fragmentation decreased significantly in both groups after treatment with hpFSH. FSH serum levels were expectably higher at the end of the treatment than before hpFSH was administered to both groups. Remarkably, TT serum levels only increased significantly in Group A. Finally, testicular volume was significantly higher in Group A after treatment, while it did not change significantly compared to baseline in Group B. The percentage of FSH responders did not differ significantly between the two groups (8/24 vs. 6/24). The daily administration of hpFSH 75 IU seems more effective than using 150 IU three times a week. However, this therapeutic scheme implies a higher number of injections and slightly higher costs.

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“…As a neuroprotective agent, testosterone acts through multiple mechanisms, including attenuation of synaptic stripping (Bahnasy, El-Heneedy, & El-Seidy, 2018; Jones, Durica, & Jacob, 1997) and Glial Fibrillary Acidic Protein (GFAP), mediation of the central glial response (Coers, Tanzer, & Jones, 2002; Delchev & Georgieva, 2018; Jones, Coers, Storer, Tanzer, & Kinderman, 1999), and enhancement of the antioxidant enzymes (Ahlbom, Prins, & Ceccatelli, 2001), heat shock protein expression (Mancuso et al, 2018; Tetzlaff, Tanzer, & Jones, 2007; Zhang et al, 2004), ribosomal response (Kinderman & Jones, 1993), and the neurotrophin brain-derived neurotrophic factor (Verhovshek, Cai, Osborne, & Sengelaub, 2010).…”

Section: Discussionmentioning

confidence: 99%

Sex Differences and Role of Gonadal Hormones on Glutamate Level, After Spinal Cord Injury in Rats: A Microdialysis Study

Samandari

Hassanpour-Ezatti

Fakhri

et al. 2018

Basic Clin. Neurosci. J.

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Introduction: Sex differences in outcomes of Spinal Cord Injury (SCI) suggest a sex-hormone-mediated effect on post-SCI pathological events, including glutamate excitotoxicity. This study aimed to investigate the importance of gonadal hormones on glutamate release subsequent to SCI in rats. Methods: After laminectomy at T8–T9, an electrolytic lesion was applied to the spinothalamic tracts of male and female rats. Using spinal microdialysis, we assessed glutamate levels at the site of lesion in both intact and gonadectomized rats for 4 hours. In this way, we examined the sex differences in the glutamate concentrations. Results: The peak retention time of glutamate level was 10.6 min and spinal glutamate concentration reached a maximum level 40 min following SCI. In male SCI rats, gonadectomy caused a significant elevation of glutamate level (P<0.001) following injury which was maximum 40 min post-SCI as well. However, no significant alterations were seen in gonadectomized female rats. Conclusion: The significant differences in glutamate levels between both intact and gonadectomized SCI male and female rats show the sex-hormone-related mechanisms underlying the molecular events in the second phase of SCI. It seems that the role of male gonadal hormones to prevent glutamate excitotoxicity is more prominent. The exact mechanisms of these hormones on the functional recovery after SCI should be clarified in further studies.

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Testosterone and FSH modulate Sertoli cell extracellular secretion: Proteomic analysis

Cited by 26 publications

References 45 publications

Multiple signaling pathways in Sertoli cells: recent findings in spermatogenesis

Multiple signaling pathways in Sertoli cells: recent findings in spermatogenesis

Retrospective Monocentric Clinical Study on Male Infertility: Comparison between Two Different Therapeutic Schemes Using Follicle-Stimulating Hormone

Sex Differences and Role of Gonadal Hormones on Glutamate Level, After Spinal Cord Injury in Rats: A Microdialysis Study

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