Testosteron- versus Dihydrotestosteron-Effekte auf permanente Plattenepithelkarzinomzellinien des Larynx

Abstract

Whereas stimulating effects of androgenic hormones on the laryngeal mucosa and their tumours have been reported in the literature, we are faced with the highest incidence of laryngeal carcinomas in the presence of a reduced androgen signal from the testes associated with aging. The discrepancies between reports in the literature and our own recent experiences with in vitro application of testosterone on permanent laryngeal squamous carcinoma cell lines, initiated this current examination of testosterone, dihydrotestosterone (DHT) and cyproterone acetate effects on two different laryngeal cancer cell lines. No DHT and cyproterone acetate effects on the androgen receptor negative line UM-SCC11B were found. However, growth of the HEp-2 line was significantly inhibited undergoing the cyproterone acetate application and significantly enhanced after DHT application. Both lines underwent a dose-dependent growth inhibition after testosterone application. These effects seem to be cytostatic rather than cytotoxic. The mechanisms leading to these effects can only be discussed hypothetically at present. Furthermore, if one takes into consideration the decrease of serum testosterone levels in aging males and the near normal levels of DHT in serum and tissues, so one may assume an imbalance between testosterone and DHT as an important cofactor in the genesis of laryngeal cancer. Current research knowledge on the basics of benign prostate hyperplasia, several experimental and clinical reports in the ENT literature together with our own experimental results, are leading to a new and hopeful therapeutic opportunity for the future, involving the blocking of 5 alpha reductase as the enzyme which manages the DHT formation from testosterone.(ABSTRACT TRUNCATED AT 250 WORDS)

Hormonal markers of aging in men with laryngeal carcinoma

“…Despite a variety of factors known to promote malignant changes in the laryngeal mucosa (eg, tobacco smoke, excessive alcohol consumption, occupational exposure to dusts, gases, and smoke4, 16–18) these factors do not explain sufficiently the imbalance between the sexes in the occurrence of the disease. So far, the hypothesis of hormone dependency of laryngeal carcinoma has not been proved 10–12, 19–22…”

“…A range of studies describing sex hormone concentrations in patients with cancer of the larynx have not brought clear results. Some authors suggested hyperandrogenism or hypoandrogenism as factors predisposing to laryngeal carcinoma; others emphasized the role of an imbalance between total testosterone and DHT 11, 21. Fillipov et al23 found low levels of total testosterone in each of 166 examined patients.…”

“…It seems especially intriguing in the context of well‐known androgen dependency of the vocal organ (resemblance with prostate cancer) 8, 9. Although the role of hormonal status in the etiology of laryngeal carcinoma has been postulated in the literature, studies performed so far have been inconclusive 10–13…”

Dihydrotestosteron- versus Gesamttestosteronwerte im Serum von Patienten mit Larynxkarzinomen und chronischer Laryngitis

“…In einer offensichtlichen Mangelsituation käme auch die Substitution von Testosteron in Frage. In Anbetracht der eher zytostatisch als zytotoxischen Wirkungen der Sexualsteroide auf die Tumorzelle [15] scheint eine Anwendung bei ausgedehnten Tumoren nicht sinnvoll. Hoffnungen können u. E. für die Behandlung von Praneoplasien bzw.…”

Der Nachweis humaner Papillomvirus (HPV) DNA in formalinfixierten invasiven Plattenepithelkarzinomen des Larynx mit der Polymerase Chain Reaction (PCR)*

“…Deitmer (1983) beobachtete zwei Typen von Frauen mit Larynxkarzinomen: ältere Frauen, die rauchten, und jungere Frauen, die Zeichen einer Virilisierung aufwiesen (6). Schon Jahrzehnte wird uber den Zusammenhang der Genese von Laiynxkarzinomen und Veränderungen der Androgen/Ostrogenbalance diskutiert (10,19). Untersuchungen an normalem Larynxgewebe, das ja hormonaktiv ist, zeigten einen Uberwiegen- Angaben ttber die Rauch-und Trinkgewohnheiten wurden der Ananmese entnommen.…”