Testis-specific and developmentally induced expression of a ghrelin gene-derived transcript that encodes a novel polypeptide in the mouse

Tanaka, Minoru; Hayashida, Yukinobu; Nakao, Nobuhiro; Nakai, Norihiro; Nakashima, Kenichiro

doi:10.1016/s0167-4781(01)00304-9

Cited by 82 publications

(38 citation statements)

References 15 publications

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“…A testis-specific and developmentally-induced ghrelin gene-derived transcript has been reported [29]. Tena-Sempere and co-workers [33] provided evidences for expression of both ghrelin and its functional receptor in the male gonad by demonstrating gene expression throughout extrauterine development of rat testis and its disappearing in experiments in which Leydig cells were selectively destroyed.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, target cells are expected to respond to both exogenous and endogenous ghrelin, the last being involved in local paracrine/autocrine regulatory mechanism modulating cell function including target cells in testis. In human and mammalian testis the gene expression for both ghrelin and GHSR-1a was evidenced [28] and the cells with ghrelin and GHSR-1a expression in testis were documented although physiological role of these proteins in seminiferous epithelium seems to need further elucidation [21,[29][30][31][32][33][34][35][36].…”

Section: Introductionmentioning

confidence: 99%

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Immunohistochemical and hybridocytochemical study on ghrelin signalling in the rat seminiferous epithelium.

Lukaszyk

Rafinska²,

P³

et al. 2010

Folia Histochem Cytobiol.

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Abstract:The results of presented study demonstrate expression of ghrelin, its functional receptor GHSR-1a and their genes in spermatogenic cells of rat testis suggesting their functioning within seminiferous epithelium. The immunohistochemical and hybrydocytochemical expression, of proteins and transcripts, was estimated taking into account the cycle of seminiferous epithelium and phases of spermatogenesis. Both transcripts and ghrelin was found to show nuclear expression and scarcely cytoplasmic. Expression of genes for ghrelin and GHSR-1a was shown in early spermatocytes and round spermatids representing transcriptional phases of meiosis and spermiogenesis. Ghrelin was evidenced to show nuclear expression in two stage-specific windows, in late spermatogonia, in spermatocytes up to early pachytenes, and again in spermatids of acrosome and early maturation phase of spermiogenesis. In late pachytenes, secondary spermatocytes, round spermatids, maturing spermatids and spermatozoa the reaction is lacking. With two types of antibodies against the GHSR-1a used the two different patterns of immunostaining was evidenced suggesting two isoforms of GHSR-1a. The first evidenced GHSR1a in cytoplasm of spermatocytes, cell membrane and acrosomes of spermatids, Sertoli cell processes and heads of spermatozoa. With second type of antibodies the immunostaining marks all steps of evolution of acrosome in spermatids. It is believed that site of ghrelin expression in seminiferous epithelium may indicate its role in local regulations, not excepting the intracellular signalling. Immunostaining pattern for GHSR-1a seems to suggest both its participation in the cross-talk among the cells and also process of furnishing gametes with GHSR-1a for its response to ghrelin in seminal plasma or female reproductive tract.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Immunohistochemical and hybridocytochemical study on ghrelin signalling in the rat seminiferous epithelium.

Lukaszyk

Rafinska²,

P³

et al. 2010

Folia Histochem Cytobiol.

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“…Another splicing variant was detected in the mouse testis (Tanaka et al, 2001b). This variant, a ghrelin gene-derived transcript (GGDT), comprises the 68-bp 5'-unique sequence located between exons 3 and 4 of the ghrelin gene, and the remaining 252 bp sequence identical to the exons 4 and 5 of mouse ghrelin gene.…”

Section: Ghrelin Gene-derived Transcriptsmentioning

confidence: 99%

Ghrelin, a Gastric Hormone with Diverse Functions

Li¹,

Luo²,

Li³

et al. 2012

Chemical Biology

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“…Tanaka et al (2001) identified a testis-specific ghrelin gene derived transcript in the mouse. This shows that ghrelin gene expression is ubiquitous and in this case this alternatively spliced variant could play a role in local control of spermatogenesis and in the synthesis of testosterone.…”

Section: Control Of the Hypothalamic-pituitary Axes: Human Studiesmentioning

confidence: 99%

Ghrelin and reproductive disorders

Repaci

Gambineri

Pagotto

et al. 2011

Molecular and Cellular Endocrinology

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Ghrelin is an important factor involved in most of the metabolic and hormonal signals which adapt the reproductive functions in conditions of altered energy balance. Moreover, the coordinated role of leptin and ghrelin appears in fact to have a specific role in the regulation of puberty. Systemic action of ghrelin on the reproductive axis involves the control of the hypothalamic-pituitary-gondal axis. In addition, it has been shown that ghrelin may directly act at a gonadal level in both females and males. Available data also demonstrate that sex steroid hormones and gonadotropins may in turn regulate the gonadal effect of ghrelin, as documented by studies performed in females with the polycystic ovary syndrome and in hypogonadal men. Notably, recent studies also confirm a potentially important role for ghrelin in fetal and neonatal energy balance, and specifically in allowing fetal adaptation to an adverse intrauterine environment.

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Testis-specific and developmentally induced expression of a ghrelin gene-derived transcript that encodes a novel polypeptide in the mouse

Cited by 82 publications

References 15 publications

Immunohistochemical and hybridocytochemical study on ghrelin signalling in the rat seminiferous epithelium.

Immunohistochemical and hybridocytochemical study on ghrelin signalling in the rat seminiferous epithelium.

Ghrelin, a Gastric Hormone with Diverse Functions

Ghrelin and reproductive disorders

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