Testing reticulate evolution of fourVitisspecies from East Asia using restriction‐site associated DNA sequencing

Abstract

Reticulate evolution is an important driving force of angiosperm evolution. It has been proposed as an important evolutionary process in Vitis L. subgenus Vitis. Events of natural hybridization and introgression of several taxa native to North America have been hypothesized and discussed. However, there is no convincing evidence of reticulate evolution reported for closely related Vitis taxa from East Asia. We aim to explore natural hybridization and introgression among four closely related Vitis taxa from East Asia (V. amurensis Ruprecht, V. romanetii Romanet du Caillaud, V. shenxiensis C. L. Li, and V. piasezkii Maximowicz) with the restriction‐site associated DNA sequencing technique. A total of 46 accessions, covering the potential morphological and geographic variation of each species, are sequenced. Our results show a complex evolutionary pattern of the four Vitis species. The phylogenetic inference suggests that V. amurensis is monophyletic, however, V. romanetii, V. shenxiensis, and V. piasezkii do not appear to be monophyletic. Significant signals of introgression in some accessions have been detected by population structure analyses. D‐statistics analysis and population structure analyses support the presence of introgression between V. shenxiensis/V. piasezkii and V. romanetii in sympatric populations, but a strong signal of admixture has not been recognized between distantly located populations. Our results provide strong evidence of reticulate evolution among V. romanetii, V. shenxiensis, and V. piasezkii.

On the recognition of the long neglected Vitis adenoclada Hand.-Mazz. (Vitaceae) from southern China

“…We hence regard Vitis davidii as a more likely parental species than V. romanetii due to their geographic distribution and the highly similar glandular trichome morphology. Ma et al (2018Ma et al ( , 2020 also showed that Vitis adenoclada and V. davidii grouped in one clade in the nuclear phylogeny, consistent with our hypothesis.…”

“…Li (1998) and Ren and Wen (2007) did not treat Vitis adenoclada Hand.-Mazz., which was described from Hunan by Han-del-Mazzetti (1925), in the Flora Reipublicae Ropularis Sinicicae and Flora of China treatments. Species delimitation of eastern Asian Vitis remains highly problematic (Li et al 1996;Wan et al 2008;Ma et al 2018Ma et al , 2020. Based on our recent field work and the examination of the type material and additional specimens, we herein propose to recognize the long-neglected Vitis adenoclada from southern China.…”

Back to the Origins: Background and Perspectives of Grapevine Domestication

“…The TreeMix model [ 117 ], developed to address historical relationships, showed a good efficiency when employed to resolve the evolution of crops and their wild relatives [ 118 , 119 ], but also unravelled the intricate relationship between grape hybrids [ 81 ]. Furthermore, the D-statistics analysis (ABBA-BABA tests) have already been applied to detect introgression among sympatric populations of Asian grapes providing interesting findings [ 120 ] while inferential tools for historical demography such as Approximate Bayesian Computation (ABC) methods could be used to estimate different demographic parameters such as divergence times and migration rates.…”

Nuclear and Chloroplast Sequences Resolve the Enigmatic Origin of the Concord Grape

“…Wan et al, 2013;Liu et al, 2016;Ma et al, 2018a;Wen et al, 2018a). Nevertheless, several studies have resolved Vitis subgenus Vitis into two main clades corresponding to their geographic distributional areas in Eurasia and North America (e.g., Zecca et al, 2012;Klein et al, 2018;Ma et al, 2018b;Wen et al, 2018a).…”