Testing of antibiotic combinations in NDM-1-producing nosocomial carbapenem resistant Acinetobacter baumannii

Shabayek, Shimaa Mahmoud; El-damasy, Dalia A.; Hassanin, Omayma M.; Radwan, Sahar

doi:10.21608/aijpms.2021.52377.1007

Cited by 2 publications

(2 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, no antagonism activity was detected. Shabayek, El-Damasy [52] demonstrated an additive effect between meropenem and amikacin in the treatment of multi-drug resistant Acinetobacter. The combination efficacy of meropenem and amikacin against metallo-βlactamases producing Acinetobacter strains was discussed by Esadoglu, Ozer [53].…”

Section: Discussionmentioning

confidence: 99%

Comparative Investigation into the Roles of Imipenem:Cyclodextrin Complexation and Antibiotic Combination in Combatting Antimicrobial Resistance in Gram-Negative Bacteria

Farhan,

El-Baky,

Ahmed

et al. 2023

Pharmaceuticals

View full text Add to dashboard Cite

Extensively drug-resistant (XDR), multidrug-resistant (MDR) and pandrug-resistant (PDR) Gram-negative microorganisms (GNBs) are considered a significant global threat. β-lactam and aminoglycoside combinations and imipenem:cyclodextrin inclusion complexes were studied for the treatment of lethal GNBs. This is because of the broad empiric coverage of the two drugs and their possession of different spectra of activity. Two cyclodextrins (β- and hydroxy propyl β-cyclodextrins) were utilized for inclusion complex formation with imipenem using the physical and kneading methods. In silico investigation using the molecular docking and Fourier-infrared spectroscopy (FTIR) were employed to estimate binding constant and confirm complex formation, respectively. The in vitro effects of amikacin and imipenem combination in comparison to the effect of imipenem-β- and hydroxy propyl β-cyclodextrin (CD) complexes against Klebsiella spp. and Acinetobacter baumannii were studied. The isolated microorganisms’ antimicrobial responsiveness to various antibiotics (19 antibiotics) was evaluated. It was found that piperacillin/tazobactam and gentamycin (resistance rates were 33.3% and 34%, respectively) were the most effective antimicrobials. The in vitro studies have been performed by the checkerboard technique and time-killing assay. The studied combination of amikacin and imipenem showed a substantial drop in bacterial count (p < 0.05). The in vitro studies demonstrated a synergism for the investigated combination. Conventional PCR was used in molecular studies to identify the resistance genes bla IMP and aac (6′)-Ib. The blaIMP and aac (6′)-Ib were recorded in 38.2% and 3.6% of the studied isolates, respectively. The in vitro studies showed synergistic effects among the tested antibiotics with FICIs of ≤0.5. Finally, the study compared the reduction in bacterial count between the tested antibiotic combinations and imipenem:CD physical and kneaded mixtures. Imipenem:CD inclusion complexes demonstrated a significant bacterial count reduction over the antibiotic combination. These results highlight the emerging role of CDs as safe biofunctional excipients in the combat against superbug bacterial resistance.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparative Investigation into the Roles of Imipenem:Cyclodextrin Complexation and Antibiotic Combination in Combatting Antimicrobial Resistance in Gram-Negative Bacteria

Farhan,

El-Baky,

Ahmed

et al. 2023

Pharmaceuticals

View full text Add to dashboard Cite

show abstract

“…NDM-1 were classified as superbugs that were reportedly known to be impossible to treat. Using one of the detection techniques in a previous study performed in Egypt, the percentage of presence of NDM-1 gene was 44.44% of the MBLs producers (76).…”

Section: Resistancementioning

confidence: 94%

Acinetobacter baumannii: An Overview of Virulence Factors, Resistance Mechanisms and Treatment Options of the Emerging Pathogen

Badie,

Basyony,

El-damasy

et al. 2024

ERU Research Journal

View full text Add to dashboard Cite

In the current healthcare system, Acinetobacter baumannii is unquestionably one of the most aggressive bacteria causing nosocomial infections including pneumonia, bacteremia and wound infections. The frequent occurrence of Acinetobacter baumannii infections and outbreaks highlights the urgent requirement for using efficient antimicrobial agents to treat such cases due to the availability of few effective ones. This prompted the Centers for Disease Control and Prevention (CDC) to consider Acinetobacter baumannii as an urgent threat. Recent research on the virulence factors and resistance mechanisms of Acinetobacter baumannii is necessary to comprehend and combat this threat. Herein, we overviewed different virulence factors and resistance mechanisms involved in the pathogenesis of Acinetobacter baumannii. Virulence factors include outer membrane protein A (porins), biofilm formation, metal acquisition systems and lipopolysaccharide whereas antimicrobials resistance mechanisms involve β-lactamases, aminoglycosides-modifying enzymes, efflux systems, reduced membrane permeability and alteration of the target site of the antibiotics. Finally, a summary of potential and innovative therapies for infections caused by Acinetobacter baumannii was discussed.

show abstract

Testing of antibiotic combinations in NDM-1-producing nosocomial carbapenem resistant Acinetobacter baumannii

Cited by 2 publications

References 5 publications

Comparative Investigation into the Roles of Imipenem:Cyclodextrin Complexation and Antibiotic Combination in Combatting Antimicrobial Resistance in Gram-Negative Bacteria

Comparative Investigation into the Roles of Imipenem:Cyclodextrin Complexation and Antibiotic Combination in Combatting Antimicrobial Resistance in Gram-Negative Bacteria

Acinetobacter baumannii: An Overview of Virulence Factors, Resistance Mechanisms and Treatment Options of the Emerging Pathogen

Contact Info

Product

Resources

About