Testing of anti-human lymphocyte sera in chimpanzees and lower primates

Balner, H.; Eysvoogel, V. P.; Cleton, F. J.

doi:10.1097/00007890-196807000-00025

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“…The GBMRAb appeared as early as the 21st day and persisted in most animals studied serially. There was no correlation between the presence of GBMRAb and the in vivo immunosuppressive effect reported to us by the suppliers of 20 ALG's tested (Balner [18]) (Table III).…”

Section: Resultsmentioning

confidence: 72%

Glomerular basement membrane—reactive antibody in anti-lymphocyte globulin

Wilson¹,

Dixon²,

Fortner³

et al. 1971

J. Clin. Invest.

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A B S T R A C T Equine immunoglobulin was detected along the glomerular basement membrane of three human homograft recipients who had been treated with equine anti-lymphocyte globulin. Anti-lymphocyte globulins, given these patients, were obtained by immunization of horses with lymphocytes from human spleens and/or lymph nodes and contained glomerular basement membrane-reactive antibodies. Quantitative paired-label isotope experiments (in rats) demonstrated that 30-170 IPg/ml of kidney-fixing antibodies were present in these preparations. The anti-lymphocyte globulins formed a line of identity with a sheep anti-human glomerular basement serum when reacted against collagenase-solubilized human glomerular basement membrane in double diffusion in agar. The renal fixation of these antibodies was blocked by absorption with human glomerular basement membrane, but not by buffy-coat leukocytes, indicating that they were directed specifically toward antigens in the basement membrane and were not crossreacting anti-lymphocyte antibodies.Anti-lymphocyte globulin preparations for human use were studied for glomerular basement membrane-reactive antibodies by a direct immunofluorescent assay in rats. Anti-lymphocyte globulin from 13 of 20 horses, and 7 of 10 serum pools from horses immunized with lymphocytes derived from solid lymphoid organs (spleen, thymus, lymph node, tonsil), contained glomerular basement membrane-reactive antibodies. Sera from 18 horses injected with thoracic duct cells or cultured lymphoblasts This is publication 465 from the

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Section: Resultsmentioning

confidence: 72%