Testing biological activity of model Maillard reaction products: studies on gastric smooth muscle tissues

Abstract

Water-soluble Maillard reaction products obtained from five different model systems were investigated for their effects upon the mechanical activity of rat gastric smooth muscle. Most of the total Maillard reaction products applied at concentration of 1.5 mg/ml evoked contractions; among them the product obtained from arginine and glucose (Arg-Glc) produced the most powerful contractions. The product obtained from glycine and ascorbic acid (Gly-AsA) was the only one that brought about relaxation response. The high molecular weight fractions (>3,500 Da) isolated from the reaction systems Arg-Glc and Gly-AsA demonstrated effects similar in type and amplitude to those evoked by non-fractioned reaction products. The results obtained suggest that moieties of molecules acting upon the muscle tonus originate mainly from lysine and arginine residues; that these structures are available in both low and high molecular pools in similar concentrations, and most likely these fragments act upon membrane-located cellular structures involved in calcium transport.

Effects of Coffee and Its Components on the Gastrointestinal Tract and the Brain–Gut Axis

“…Moreover, melanoidins did not significantly alter the latency of expulsion of a bead inserted 3 cm into the colorectum, suggesting that the motor agents (intrinsic and extrinsic innervation, smooth muscle, and ICCs) involved in the colonic propulsion at this level were not altered by dietary exposure to coffee silverskin-derived melanoidins and that these may have potential to be used as a functional food ingredient [178]. Interestingly, Argirova et al (2010) [185] showed that melanoidins act upon muscle tone and might facilitate Ca 2+ influx into the cells of isolated gastric muscle layers. Thus, these compounds might exert their pro-motility action not only through a fiber effect, but also through the direct activation of gastrointestinal smooth muscle cells, which needs to be confirmed to occur also using isolated intestinal muscle tissue.…”

Melanoidins produced by the Maillard reaction: Structure and biological activity

“…Brown MRPs at low concentrations provoked concentration-dependent contraction, whereas brown MRPs at high concentrations induced muscle relaxation (Argirova, Stefanova, Krustev, & Turiiski, 2010).…”

Impact of freeNε-carboxymethyllysine, its precursor glyoxal and AGE-modified BSA on serotonin release from human parietal cells in culture

“… 37 In addition, several studies showed comparable effects of free and protein-linked CML in different cell lines. 10 , 11 As CML formation in the stomach has been reported to increase in the presence of catechols, 20 and several Maillard reaction products have been indicated to exert influence on the mechanical activity of the gastric smooth muscle, 17 we assessed the effect of CML on human parietal cells in culture, deriving from an adenocarcinoma of the stomach (HGT-1 cells). Furthermore, we have recently shown this cell line to be capable of serotonin biosynthesis and release upon stimulation.…”

“…In a study by Morioka et al , 16 positive Glycer-AGE immunostaining was observed in the stomachs of healthy rats. Argirova and colleagues 17 assessed the effects of water-soluble Maillard reaction products (MRPs), derived from different model systems, on the mechanical activity of the gastric smooth muscle from rats. Several of the compounds applied in this work led to contractions, among which the reaction products formed between glucose and arginine produced the strongest effect.…”