Testing a Method for Evaluation of the Viability of Biofilm-Forming Bacteria after Exposure to Disinfectants

Nemchenko, U. M.; Voropaeva, N. M.; Sitnikova, K. O.; Belkova, N. L.; Savilov, E. D.

doi:10.1007/s10517-023-05967-z

Cited by 1 publication

(1 citation statement)

References 13 publications

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“…Surfactants' impact on biofilm development was tested against T20, T80, and SDS, at concentrations of 1%, 2%, and 5%, and 0.1%, 0.2%, and 0.5%, respectively. Researchers have frequently employed the reduction of tetrazolium salts to gauge the metabolic activity of microbial biofilms across various experimental contexts [39,40,[43][44][45][46][47]. This technique has revealed a consistent correlation between metabolic activity and the reduction of tetrazolium salts.…”

Section: Resultsmentioning

confidence: 99%

Surfactants’ Interplay with Biofilm Development in Staphylococcus and Candida

Aonofriesei

2024

Pharmaceutics

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The capacity of micro-organisms to form biofilms is a pervasive trait in the microbial realm. For pathogens, biofilm formation serves as a virulence factor facilitating successful host colonization. Simultaneously, infections stemming from biofilm-forming micro-organisms pose significant treatment challenges due to their heightened resistance to antimicrobial agents. Hence, the quest for active compounds capable of impeding microbial biofilm development stands as a pivotal pursuit in biomedical research. This study presents findings concerning the impact of three surfactants, namely, polysorbate 20 (T20), polysorbate 80 (T80), and sodium dodecyl sulfate (SDS), on the initial stage of biofilm development in both Staphylococcus aureus and Candida dubliniensis. In contrast to previous investigations, we conducted a comparative assessment of the biofilm development capacity of these two taxonomically distant groups, predicated on their shared ability to reduce TTC. The common metabolic trait shared by S. aureus and C. dubliniensis in reducing TTC to formazan facilitated a simultaneous evaluation of biofilm development under the influence of surfactants across both groups. Our results revealed that surfactants could impede the development of biofilms in both species by disrupting the initial cell attachment step. The observed effect was contingent upon the concentration and type of compound, with a higher inhibition observed in culture media supplemented with SDS. At maximum concentrations (5%), T20 and T80 significantly curtailed the formation and viability of S. aureus and C. dubliniensis biofilms. Specifically, T20 inhibited biofilm development by 75.36% in S. aureus and 71.18% in C. dubliniensis, while T80 exhibited a slightly lower inhibitory effect, with values ranging between 66.68% (C. dubliniensis) and 65.54% (S. aureus) compared to the controls. Incorporating these two non-toxic surfactants into pharmaceutical formulations could potentially enhance the inhibitory efficacy of selected antimicrobial agents, particularly in external topical applications.

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Section: Resultsmentioning

confidence: 99%

Surfactants’ Interplay with Biofilm Development in Staphylococcus and Candida

Aonofriesei

2024

Pharmaceutics

View full text Add to dashboard Cite

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Testing a Method for Evaluation of the Viability of Biofilm-Forming Bacteria after Exposure to Disinfectants

Cited by 1 publication

References 13 publications

Surfactants’ Interplay with Biofilm Development in Staphylococcus and Candida

Surfactants’ Interplay with Biofilm Development in Staphylococcus and Candida

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