Testiculo Protective Effects of Ethanolic Roots Extract of Pseudocedrela kotschyi on Alloxan Induced Testicular Damage in Diabetic Rats

Ojewale, A.O.; Olaniyan, O. T.; Faduyile, Francis Adedayo; Odukanmi, Olugbenga Adeola; Oguntola, J. A.; Dare, B. J.

doi:10.9734/bjmmr/2014/5680

Cited by 10 publications

(9 citation statements)

References 25 publications

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“…These results suggested that the MLT might have a free radical scavenging activity and prevents pathological alteration caused by O 2 − and OH − . Indeed, similar findings have been reported on testis tissue that alloxan injection led to lower GSH and SOD activities [35,36] . The DM is characterized by a sudden and distinct loss in body weight, a symptom as also observed herein.…”

Section: Discussionsupporting

confidence: 85%

Melatonin Ratlarda Alloxan İle İndüklenen Diyabet Sonrası Oluşan Testiküler Hasarı ve Oksidatif Etkileri Hafifletir

Akman¹,

Şengül²,

Özkanlar³

et al. 2015

Kafkas Univ Vet Fak Derg

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The aim of this study was to examine the protective effect of melatonin (MLT) on the alloxan-induced post-diabetic testicular damage in rats. Forty-eight Sprague Dawley male rats were divided into four groups (n=12). Group C received 0.9% saline for 45 days, as control. Group DM (Diabetes Mellitus) was injected by 120 mg/kg single dose alloxan intraperitoneally (IP). Group MLT was treated by 10 mg/ kg/d MLT IP for 45 d. Group DM+MLT was treated by 10 mg/kg/d MLT IP for 45 days following alloxan injection. Six animals on days 15 and the remaining 6 on days 45 were sacrificed in each group after the initiation of the study. Testis tissues and blood samples were collected to investigate blood glucose, oxidant and antioxidant statues, sperm parameters, and histopathological and 8-hydroxy-2′-deoxyguanosine (8-OHdG)-immunopositive cell examinations. MLT treatment of diabetic rats prevented the increases in malondialdehyde (MDA) levels and 8-OHdG concentrations, and the decreases in the GSH and SOD levels observed in DM groups. Alloxan administration depressed the sperm concentration, the number of progressively motile spermatozoa, premature acrosome reaction (AR) and calcium ionophore A23187-induced AR rates, without any change in dead and/or abnormal sperm rates in DM group. Histopathological and immunohistochemical observations also revealed that MLT-treated diabetic rats had an enhanced histological architecture and DNA damage in testis. These findings suggest that MLT treatment prevents the testicular damage by declining the oxidative stress, but it did not recover the depressed sperm parameters in diabetic rats. Keywords: Diabetes, Melatonin, Rat, Testis Melatonin Ratlarda Alloxan İle İndüklenen Diyabet Sonrası Oluşan Testiküler Hasarı ve Oksidatif Etkileri Hafifletir ÖzetBu çalışmanın amacı, ratlarda alloxan ile oluşturulan diyabet sonrası gözlenen testiküler hasar üzerine melatonin'in (MLT) koruyucu etkisini incelemekti. Araştırmada kullanılan 48 adet Sprague Dawley ırkı erkek rat 4 gruba ayrıldı (n=12). Kontrol grubu olan Grup C'ye 45 gün süreyle %0.9 serum fizyolojik verildi. Grup DM'ye (Diabetes Mellitus) tek doz alloxan (120 mg/kg) intraperitoneal (IP) olarak enjekte edildi. Grup MLT'ye 45 gün süreyle 10 mg/kg/gün dozda MLT IP olarak enjekte edildi. Grup DM+MLT'ye alloxan enjeksiyonunu takiben 45 gün süreyle 10 mg/kg/gün dozda MLT IP olarak uygulandı. Her gruptan 6 hayvana araştırma başladıktan 15 gün sonra, diğer 6 hayvana ise 45 gün sonra ötenazi uygulandı. Kan glikoz seviyesi, oksidan ve antioksidan durumları, sperm parametreleri ve histopatolojik ile 8-hydroxy-2′-deoxyguanosine (8-OHdG)-immunopozitif hücre incelemeleri için testis dokusu ve kan örnekleri alındı. Diyabetik ratlara yapılan MLT uygulamasının, grup DM'de görülen malondialdehyde (MDA) seviyesi ile 8-OHdG yoğunluğundaki artışı ve glutathion (GSH) ile superoxide dismutase (SOD) seviyelerindeki azalmayı önlediği belirlendi. Alloxan uygulaması grup DM'de sperm yoğunluğu, motil sperm sayısı, prematüre akrozom reaksiyonu (AR) ve calcium io...

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Section: Discussionsupporting

confidence: 85%

Melatonin Ratlarda Alloxan İle İndüklenen Diyabet Sonrası Oluşan Testiküler Hasarı ve Oksidatif Etkileri Hafifletir

Akman¹,

Şengül²,

Özkanlar³

et al. 2015

Kafkas Univ Vet Fak Derg

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“…This disparity demonstrates that liver problems mainly come from a HFD consumed over a long period [15]. The significant increase of ALP and AST may be due to the damage and leakage of enzymes through cellular membranes [26]. It may also be caused by the damage to the lysosomal membrane from the lysosome in the extracellular fluid [26].…”

Section: Discussionmentioning

confidence: 99%

“…The significant increase of ALP and AST may be due to the damage and leakage of enzymes through cellular membranes [26]. It may also be caused by the damage to the lysosomal membrane from the lysosome in the extracellular fluid [26]. Interestingly, the levels of ALP, AST, and ALT in all SBH-treated groups (500 mg/kg, 750 mg/kg, and 1000 mg/kg) are between the levels of normal rats and control obese rats.…”

Section: Discussionmentioning

confidence: 99%

Supplementation of Stingless Bee Honey from Heterotrigona itama Improves Antiobesity Parameters in High‐Fat Diet Induced Obese Rat Model

Rafie

Syahir

Ahmad

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Heterotrigona itama is a common stingless bee species found in Southeast Asia. Studies on the health benefits of its honey are limited in comparison with other stingless bee species. This study examines the antiobesity benefits found in stingless bee honey (SBH) from H. itama. The parameters used to measure the benefits were weight change, morphological structures, and biochemical characteristics. The research was conducted by using rats that were given a high-fat diet (HFD). In total 48 male Sprague Dawley (SD) rats were given a formulated HFD to increase the levels of obesity, the HFD was administered with a value of 0.68 g/cm2. The duration of the treatment was six weeks, and the results show that the induction obesity using the HFD was successful. Following this, the rats were then treated with SBH (at dosages of 1000 mg/kg, 750 mg/kg or 500 mg/kg), with orlistat or with a placebo. Compared with typical obesity treatment methods, the one that used the three dosages of SBH showed a higher reduction in body mass index (BMI), percentage of body weight gain, adiposity index, and relative organ weight (ROW). The levels of liver enzymes (ALT, AST, and ALP) were also significantly lower in SBH-treated groups. The levels of triglycerides and LDL-cholesterol were significantly lower, while the level of HDL-cholesterol was significantly higher in comparison with the control obese group. In terms of morphological structures, the number of adipocyte cells was reduced, and the hepatocytes found in the liver were less prone to rupturing when treated with SBH. In conclusion, the administration of SBH led to an improvement in indicators associated with obesity reduction. SBH also possesses a hepatoprotective potential which can reduce the health risks related to obesity.

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“…[34] Our data showed that STZinduced diabetic rats have severe loss in body weight which is consistent with the findings of other investigators. [35,36] This marked reduction in body weight may be breakdown of structural tissue protein in diabetic state. Diabetes causes remarkable increase in skeletal muscle catabolism along with reduction in protein biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

Untitled

Maiti¹

2017

IJGP

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Background: Tamarindus indica Linn. (Caesalpiniaceae) is a well-known traditional plant used by the tribes as well as rural sectors of tropical countries. Locally, it is named as Tantul and recognized as therapeutic uses of different diseases. Aim: The present study aimed to evaluate the efficacy of hydromethanolic extract of T. indica Linn. seed in streptozotocin (STZ)-induced diabetic testicular dysfunction correction. Materials and Methods: STZ-induced diabetic state has been established here by single intramuscular injection of STZ (6 mg/100 g body weight in citrate buffer, pH 4.5) resulted hyperglycemia. STZ-induced experimental diabetes resulted testicular dysfunctions evaluated by sperm count, sperm motility, viability, and seminal vesicle fructose content as well as by the level of serum testosterone. Testicular oxidative stress has been observed by the monitoring of catalase, peroxidase, superoxide dismutase, and glutathione-S-transferase activities. Quantification of thiobarbituric acid reactive substances and conjugated diene in testicular tissue was assessed. General and metabolic toxicity assessment parameters were studied. Results: Administration of hydromethanolic extract to STZ-induced diabetic rat at the dose of 80 mg/100 g body weight/day for 28 days resulted a significant protection in fasting blood glucose, serum insulin, and serum testosterone levels (P < 0.05) along with the correction of testicular above-mentioned parameters as well as semen analysis parameters toward the control level (P < 0.05). This extract has no general toxic effect on the body weight, along with no metabolic toxicity that was reflected here by the assessment of serum glutamate oxaloacetate transaminase and pyruvate transaminase activities. Conclusion: These data demonstrated that T. indica significantly improved STZ-induced diabetic complication in rat testis. This study suggested that hydromethanolic extract might have a protective role against oxidative stress-induced impairment in testicular functions in diabetic rats.

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Testiculo Protective Effects of Ethanolic Roots Extract of Pseudocedrela kotschyi on Alloxan Induced Testicular Damage in Diabetic Rats

Cited by 10 publications

References 25 publications

Melatonin Ratlarda Alloxan İle İndüklenen Diyabet Sonrası Oluşan Testiküler Hasarı ve Oksidatif Etkileri Hafifletir

Melatonin Ratlarda Alloxan İle İndüklenen Diyabet Sonrası Oluşan Testiküler Hasarı ve Oksidatif Etkileri Hafifletir

Supplementation of Stingless Bee Honey from Heterotrigona itama Improves Antiobesity Parameters in High‐Fat Diet Induced Obese Rat Model

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