Testicular toxicity of boric acid (BA): Relationship of dose to lesion development and recovery in the F344 rat

Ku, Warren W.; Chapin, Robert E.; Wine, Robert N.; Gladen, Beth C.

doi:10.1016/0890-6238(93)90020-8

Cited by 135 publications

(85 citation statements)

References 29 publications

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“…In the last 20 years, several researches have shown that Boron may have some positive effects for human health (Ku et al, 1993;Brown et al, 1989). In addition, it has been shown that Boron has many regulative roles in macromineral metabolism (Brown et al, 1989;Hegsted et al, 1991), energy metabolism (Hunt et al, 1997) and on the immune system (Hunt and Idso, 1999).…”

mentioning

confidence: 99%

Dietary Boron Supplementation and its Impact on Semen Characteristics and Physiological Status of Adult Male Rabbits

Elkomy¹,

El-Hady²,

Elghalid³

2015

Asian J. of Poultry Science

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This study was carried out to assess the effect of Boron supplementation on male rabbit's reproductive performance. A total of 20 V. Line (V.L) adult rabbit bucks were divided into four equal experimental groups (5 bucks for each). Groups 2, 3 and 4 were fed rations supplemented with 17.5, 35 and 70 mg boron/kg feed in the form of boric acid (100, 200 and 400 mg boric acid, respectively) over 8 weeks. The first group was served as a control group. Results obtained by the Boron groups revealed that adding Boron to rabbit bucks rations at any tested doses resulted in a non-significant increase in the ejaculate volume of male rabbit by approximately 25% above the control group. Adding Boron at any dose exhibited a significant increase (p#0.01) in sperm concentration, total sperm output, sperm motility, live sperm and normal sperm compared to the control group and the high Boron dose had the highest effect on previous measurements compared to the low and medium Boron doses. Opposite trend was shown in the reaction time (libido) and seminal plasma fructose concentration which showed a significant decrease in these parameters in the Boron treated groups compared to the control. Plasma total protein, glucose, total lipids and triglycerides concentration were boosted in the Boron groups compared to the control (p#0.01) for glucose and triglycerides measurements. On the contrary, treated male rabbits with Boron at any tested doses resulted in a significant reduction in plasma cholesterol concentration. Blood and seminal plasma AST and ALT enzymes activities showed a tendency to decrease by Boron treatments compared to the control group. Treating bucks by different Boron doses resulted in a significant increase (p#0.01) in Total Antioxidant Capacity (TAC) to reach 110, 128 and 163% of blood plasma and 114, 121 and 122% of seminal plasma compared to the control value. On the other side, adding Boron to bucks rations did not affect the activity of seminal plasma alkaline phosphatase enzyme. Blood testosterone hormone had a tendency to increase in the Boron groups compared to the control group but this increase was not significant. It could be concluded that feeding rabbit bucks on rations containing the studied Boron doses results in a significant improvement in semen quality characteristics and had a positive effect on their physiological status.

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confidence: 99%

Dietary Boron Supplementation and its Impact on Semen Characteristics and Physiological Status of Adult Male Rabbits

Elkomy¹,

El-Hady²,

Elghalid³

2015

Asian J. of Poultry Science

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“…These include phthalate esters (Creasy et al, 1985;Creasy, 2001;Moffit et al, 2007;Richburg and Boekelheide, 1996), 1,3-dinitrobenzene (Hess, 1990) and methyl 2-benzimidazole carbamate (Correa and Miller, 2001;Nakai and Hess, 1997;Winder et al, 2001), all indicating the suppression of androgen production. Similar effects produced by boric acid (Ku et al, 1993;Kudo et al, 2000), halogenated acid (Linder et al, 1990(Linder et al, , 1994, methyl methanesulfonate (Kuriyama et al, 2005;Ozawa et al, 2000;Richburg and Boekelheide, 1996) and tributylin chloride (Omura et al, 2001) indicate estrogenlike effects. Failure of spermiation has also been shown in primates including men treated with hormonal contraceptives (McLachlan et al, 2002;O'Donnell et al, 2001;Russell, 1977).…”

Section: Discussionmentioning

confidence: 75%

Testicular spermiation failure in rats exposed prenatally to 3,3',4,4',5-pentachlorobiphenyl

et al. 2010

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-Testicular spermatogenesis was studied in 7-, 10-, 13-and 17-week-old Sprague-Dawley rats whose dams had been administered intragastrically with 2.5, 25, or 250 ng of 3,3′,4,4′,5-pentachlorobiphenyl (PCB126) or vehicle on days 13-19 of gestation. The 250 ng groups among the 7-, 10-and 13-week-old offspring showed significant inhibition of mature spermatid release (spermiation), but 17-weekold offspring did not show this. These alterations were not observed in other PCB126 and vehicle groups, and no germ cell or Sertoli cell degeneration were observed in any group. Spermiation failure at puberty appeared in those rats born to dams exposed 250 ng/kg PCB126 on days 13-19 of gestation was reversible change that recovered at adulthood. Because the serum testosterone, luteinizing hormone and folliclestimulating hormone concentrations were similar in the PCB126 and vehicle groups, a direct endocrine cause for the observed effects was unlikely.

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“…Several small pilot studies were performed to ascertain that boric acid would affect rats, and to estimate appropriate sacrifice times. For the definitive study, 6 In preliminary pilot studies, three rats/group/time consumed diet containing 2000, 3000, 4500, 6000, and 9000 ppm BORIC ACID, and were killed at weekly intervals for up to 9 weeks. In the definitive study, young adult male rats were exposed to boric acid-containing powdered feed at 0, 3000, 4500, 6000, and 9000 ppm, and 6 rats from each group were sacrificed at weekly intervals for 9 weeks (6 10 3. returned to pelleted control feed after the 9-week exposure, and sacrificed after 1, 2, 3, and 4 cycles of spermatogenesis (8,16,24, and 32 weeks, respectively).…”

Section: Male Pathogenesis Studymentioning

confidence: 99%

The reproductive toxicity of boric acid.

Chapin

1994

Environ Health Perspect

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Previous studies on the reproductive toxicity of boric acid have indicated that male rodents suffer testicular atrophy after treatment. There were, however, no studies of the potential effects on female fertility or on the neonate. In addition, no study described the development of the testicular lesion, thought to be related to the mechanism of toxicity. A Reproductive Assessment by Continuous Breeding (RACB) study using mice exposed to boric acid at 1000, 4500, and 9000 ppm in the diet indicated that there are probably multiple sites of action, although male fertility appears very sensitive. Possible effects on female fertility cannot be separated from potential developmental toxicity and need additional investigation.Decrements in sperm motility were observed at all exposure levels, and testicular atrophy was confirmed in high-and middle-dose-group males.This was investigated further by timed serial-sacrifice studies using 9000 ppm in the diet of rats, which found that the first lesion seen in the testis was an inhibition of spermiation (release of mature spermatids). With continued dosing, this was followed by a disorganization of the normal ordered layering of the seminiferous epithelium, germ cell sloughing and death, and finally, atrophy. Subsequent studies using additional doses (2000, 3000, 4500, 6000, and 9000 ppm) found that it was possible to observe inhibited spermiation that did not progress to atrophy (4500 ppm and below) within the 9-week exposure period. Also, once atrophic (from 9000-and 6000-ppm exposures), there was no return of spermatogenesis after refeeding of normal diet for 8, 16, 24, or 32 weeks, despite the presence of a normal-sized population of spermatogonia that was seen to divide. No effects were observed in rats treated with 2000 ppm. Testicular boron concentrations remained steady during exposure at the same level as found in blood, and declined to background levels within 72 hr after cessation of exposure. Bone boron levels were greater than those found in blood, and maintained slightly elevated levels even 32 weeks after the cessation of exposure. These studies document the effects of boric acid on functional and structural aspects of the male reproductive system; demonstrate that the tissue concentration of boron, and not total dose, is important for the testicular toxicity; and show that there is a threshold for these effects. These studies also suggest that the potential for recovery from boric acidinduced testicular atrophy should be examined in other species to evaluate the significance of the persistent atrophy seen in the rats. -Environ Health Perspect 102(Suppl 7): 87-91 (1994)

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Testicular toxicity of boric acid (BA): Relationship of dose to lesion development and recovery in the F344 rat

Cited by 135 publications

References 29 publications

Dietary Boron Supplementation and its Impact on Semen Characteristics and Physiological Status of Adult Male Rabbits

Dietary Boron Supplementation and its Impact on Semen Characteristics and Physiological Status of Adult Male Rabbits

Testicular spermiation failure in rats exposed prenatally to 3,3',4,4',5-pentachlorobiphenyl

The reproductive toxicity of boric acid.

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