Testicular tissue cryopreservation is the preferred method to preserve spermatogonial stem cells prior to transplantation

Onofre, Jaime; Kadam, Prashant; Baert, Yoni; Goossens, Ellen

doi:10.1016/j.rbmo.2019.10.016

Cited by 22 publications

(14 citation statements)

References 35 publications

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“…We believe that these insights will have potential translational value in the development of fertility restoration strategies for childhood cancer survivors. Cryopreservation of ITT is a preferred method over isolated spermatogonial stem cells banking [24]. Handling of ITT prior to cryopreservation or in vitro culture may influence testicular architecture, endocrine function, and spermatogonial proliferation.…”

Section: Discussionmentioning

confidence: 99%

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Impact of Temperature and Time Interval Prior to Immature Testicular-Tissue Organotypic Culture on Cellular Niche

et al. 2020

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Cryopreservation of immature-testicular-tissue (ITT) prior to gonadotoxic treatment, while experimental, is the only recommended option for fertility preservation in prepubertal boys. The handling and manipulation of ITT before cryopreservation could influence the functionality of cells during fertility restoration, which this study explored by evaluating cellular niche and quality of mouse ITT subjected to various temperatures and time durations in vitro. ITT from 6-day-old mice were handled at ultraprofound-hypothermic, profound-hypothermic, and mild-warm-ischemic temperatures for varying time periods prior to 14-day organotypic culture. Viability, functionality, synaptonemal complex and chromatin remodeling markers were assessed. Results have shown that cell viability, testosterone level, and in vitro proliferation ability did not change when ITT were held at ultraprofound-hypothermic-temperature up to 24 h, whereas cell viability was significantly reduced (P < 0.01), when held at profound-hypothermic-temperature for 24 h before culture. Further, cell viability and testosterone levels in cultured cells from profound-hypothermic group were comparable to corresponding ultraprofound-hypothermic group but with moderate reduction in postmeiotic cells (P < 0.01). In conclusion, holding ITT at ultraprofound-hypothermic-temperature is most suitable for organotypic culture, whereas short-term exposure at profound-hypothermic-temperature may compromise postmeiotic germ cell yield post in vitro culture. This data, albeit in mouse model, will have immense value in human prepubertal fertility restoration research.

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Section: Discussionmentioning

confidence: 99%

“…Cryopreservation of ITT is a preferred method over isolated spermatogonial stem cells banking [ 24 ]. Handling of ITT prior to cryopreservation or in vitro culture may influence testicular architecture, endocrine function, and spermatogonial proliferation.…”

Section: Discussionmentioning

confidence: 99%

Impact of Temperature and Time Interval Prior to Immature Testicular-Tissue Organotypic Culture on Cellular Niche

et al. 2020

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“…Reliable cryopreservation methods also ensure the optimal protection of the reproductive potential by maintaining the health of SSCs within the biopsy [16]. Research has shown that the cryopreservation of the testicular biopsy tissue as a piece is preferable to the cryopreservation of the testicular cell suspension after dissociation of the biopsy as the viability of SSCs is better preserved in testicular biopsies [6]. Biopsy cryopreservation protocols do not only safeguard the viability, longevity, and proliferation rate of the SSCs but also increase their fecundity rate.…”

Section: Testis Biopsy and Cryopreservationmentioning

confidence: 99%

“…Nonetheless, before SSCT, an aliquot of the cell suspension after propagation is recommended for safety testing (e.g. infectious contamination) [6]. This step could also include a safety check for the presence of original cancer cells [25] or resorting for leukemic cells in order to increase the purity of the SSCs [27].…”

Section: Eliminating Infiltrated Cancer Cells From the Biopsymentioning

confidence: 99%

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Fathering a child after childhood cancer treatments

et al. 2022

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Among many deleterious ramifications of oncological treatments, there is permanent male infertility due to the damage to spermatogonial stem cells (SSC) in the testes after chemotherapy or irradiation. For those patients that cannot produce sperm before cancer treatment, because of prepubertal age, there are no clinical options available to father a child. To preserve fertility in childhood cancer patients, freezing of a testis biopsy is already offered before cancer treatment, while fertility treatment options using this biopsy are still under development, including spermatogonial stem cell transplantation (SSCT). SSCT requires isolation and in vitro propagation of spermatogonial stem cells from the cryopreserved biopsy, followed by autologous transplantation back to the adult cancer survivor. Given the implications of this potential stem cell therapy to recipients, their partners, and future offspring, we here aim to thoroughly appraise the state-of-the-art of SSCT focussing on safety for both patient and his future children.

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Cryopreservation of Testicular Stem Cells and Its Application in Veterinary Science

Patra

Bhaskar

Gupta

2021

Stem Cells in Veterinary Science

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Testicular tissue cryopreservation is the preferred method to preserve spermatogonial stem cells prior to transplantation

Cited by 22 publications

References 35 publications

Impact of Temperature and Time Interval Prior to Immature Testicular-Tissue Organotypic Culture on Cellular Niche

Impact of Temperature and Time Interval Prior to Immature Testicular-Tissue Organotypic Culture on Cellular Niche

Fathering a child after childhood cancer treatments

Cryopreservation of Testicular Stem Cells and Its Application in Veterinary Science

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