Testicular Sperm Retrieval in Azoospermic Men

Pantke, P.; Diemer, Thorsten; Marconi, Marcelo; Bergmann, Martin; Steger, Klaus; Schuppe, Hans‐Christian; Weidner, W.

doi:10.1016/j.eursup.2008.07.006

Cited by 25 publications

(18 citation statements)

References 63 publications

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“…Remaining 71(88.75 %) were diagnosed as non-obstructive azoospermia patients. Obstructive azoospermia accounts for 35 % to 40 % cases of azoospermic males as per larger studies (6,11,13,14) . In our study, men with obstructive azoospermia planned for surgical retrieval are only 11.25 %.…”

Section: Discussionmentioning

confidence: 75%

“…Once fertilized, the embryo is implanted into female uterus for development. (10,11) The sperm recovery rates ranges from 90% to 100% for obstructive azoospermia using any of standard surgical technique (12) , but the sperm recovery rates for non-obstructive azoospermia range from 30-63% for TESE, and 43-63% for Micro-TESE; the rate for TEFNA is approximately 47%. (17) Out of all patients of azoospermia approximately 36% are due to obstructive azoospermia.…”

Section: Introductionmentioning

confidence: 99%

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Prospective Study of Predictive Factors and Outcome in Sperm Retrieval of Azoospermic Males: Initial Experience in Tertiary Care Centre

Kumar¹,

Specialist²

2018

jmscr

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Section: Discussionmentioning

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Prospective Study of Predictive Factors and Outcome in Sperm Retrieval of Azoospermic Males: Initial Experience in Tertiary Care Centre

Kumar¹,

Specialist²

2018

jmscr

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“…Das Risiko einer permanenten endokrinen Insuffizienz (Testosterondefizit) der therapierten Patienten durch testikuläre Gewebeentnahme ist dabei real, aber insgesamt doch gering [7].…”

Section: Die Intrazytoplasmatischen Spermatozoeninjektion (Icsi) Als unclassified

“…Mit der Einführung dieser reproduktionsmedizinischen Option entwickelten sich nun neue operative Verfahren, die notwendig geworden waren, um v. a. bei Patienten mit schwersten Spermatogenesestörungen, klinisch manifestiert durch das Vorliegen einer Azoospermie, die entsprechenden Spermatozoen zur Verfügung zu stellen [7,11]. Offene operative Verfahren konkurrierten hier anfangs mit perkutanen Verfahren, wobei sich insgesamt die offenen chirurgischen Verfahren in der Hand des Urologen durchgesetzt haben [5].…”

Section: Therapie Der Azoospermieunclassified

Therapie der Azoospermie

Diemer

Hauptmann

Weidner

2011

Urologe

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The introduction of intracytoplasmic sperm injection (ICSI) into the spectrum of assisted reproductive technologies has offered men who suffer from severe disorders of spermatogenesis and azoospermia the possibility of fathering a child. Different surgical techniques can be used to extract spermatozoa from these men from either the epididymis and/or the testis. Surgical sperm retrieval offers a treatment for patients with testicular and/or obstructive azoospermia in cases where microsurgical refertilization is not an option or has already failed. Among surgical techniques that have been explored over the years, microsurgical epididymal sperm aspiration (MESA) and testicular sperm extraction (TESE) have become the most popular. Percutaneous techniques (such as TEFNA) are available but have disadvantages versus open surgical procedures. Together with cryopreservation of extracted spermatozoa, these techniques facilitate retrieval of spermatozoa for several ICSI attempts by a single surgical intervention.

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“…The introduction of testicular sperm extraction (TESE) in combination with intracytoplasmic sperm injection (ICSI) raised hope in men with azoospermia to conceive their own biological child [3]. In the management of men with NOA, it is crucially important to develop procedures capable of predicting the presence of mature sperm, assessing its quality and successfully recovering testicular sperm from patients with NOA.…”

Section: Introductionmentioning

confidence: 99%

Molecular analysis of testis biopsy and semen pellet as complementary methods with histopathological analysis of testis in non-obstructive azoospermia

Eghbali

Sadeghi

Lakpour

et al. 2014

J Assist Reprod Genet

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Purpose Non-obstructive azoospermia (NOA) is one the many causes of male infertility (10 %) resulting from testicular failure. Multiple testicular biopsies fail to find mature sperm in at least 50 % of cases Therefore; hunting for sensitive and specific biomarkers of spermatogenesis that could better determine the fertility status in NOA can lead to improved management of male infertility. Therefore, we evaluated sperm production through analyses of germ cell-specific transcripts (DAZ, TSPY1, SPTRX3 and SPTRX1) in semen and testicular biopsies of men with azoospermia. Methods We collected semen (N=83) and testis biopsies (N= 31) from men with non-obstructive azoospermia. We later extracted RNA and synthesized cDNA using washed semen precipitate and testicular tissues. We also performed seminested PCR with designed specific primers. Using H&E method, an expert pathologist performed the histopathological evaluation. Having categorized the patients into three groups based on histopathological results, we calculated the agreement between molecular results of semen and tissues with histopathological findings for each patient using Kappa statistical test. Results Molecular findings of precipitated semen and testicular tissues were in disagreement with histopathological results in most cases. Molecular analysis of testis biopsies showed significant difference (Kappa coefficient=0.009, P value= 0.894) with histopathological results; TSPY1, DAZ, SPTRX3 and SPTRX1 were respectively detected in 94 %, 94 %, 17.6 % and 52.9 % of men diagnosed with germ cell aplasia.Capsule The molecular analysis of testicular tissues and semen precipitates for the presence of germ cell-specific transcripts is a diagnostic test that could be of help in the detection of active spermatogenesis in men with non-obstructive azoospermia.

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Testicular Sperm Retrieval in Azoospermic Men

Cited by 25 publications

References 63 publications

Prospective Study of Predictive Factors and Outcome in Sperm Retrieval of Azoospermic Males: Initial Experience in Tertiary Care Centre

Prospective Study of Predictive Factors and Outcome in Sperm Retrieval of Azoospermic Males: Initial Experience in Tertiary Care Centre

Therapie der Azoospermie

Molecular analysis of testis biopsy and semen pellet as complementary methods with histopathological analysis of testis in non-obstructive azoospermia

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