Testicular sperm extraction by Trucut needle and milking of seminiferous tubules: a technique with high yield and patient acceptability

Steele, E. K.; Kelly, JD; Lewis, Sheena; McNally, J. A.; Sloan, James M.; McClure, Neil

doi:10.1016/s0015-0282(00)00655-5

Cited by 18 publications

(8 citation statements)

References 4 publications

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“…Testicular sperm retrieval and preparation (Steele et al, 2000) Brie¯y, the spermatic cord was located and 10 ml of 0.5% bupivacaine injected around it. The epididymis was then located and a 21 G butter¯y needle used to aspirate the sperm into Biggers±Whitten± Whittingham (BWW) (Biggers et al, 1971) media.…”

Section: Subjectsmentioning

confidence: 99%

An algorithm to predict pregnancy in assisted reproduction

Lewis

O’Connell²,

Stevenson

et al. 2004

Human Reproduction

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Section: Subjectsmentioning

confidence: 99%

An algorithm to predict pregnancy in assisted reproduction

Lewis

O’Connell²,

Stevenson

et al. 2004

Human Reproduction

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show abstract

“…More recently, simple sperm aspiration procedures such as testicular (TESA) 17 and percutaneous epididymal (PESA) 18 have been introduced. The introduction of Trucut needle testicular extraction 19 using a needle wider (14‐gauge) than traditional aspiration needles (19–21‐gauge) has allowed an increase in the numbers of sperm retrieved using non‐invasive techniques (mean of approximately 100,000 per retrieval). The question therefore arises that if a simple PESA or TESA are as effective in terms of retrieval rates, ICSI outcome 20 and patient satisfaction 21,22 as an open MESA with few complications 23 and neither require the expertise of a urological surgeon, why can they not be performed routinely within an IVF unit by suitably trained gynaecologists or perhaps nursing staff?…”

Section: Sperm Retrieval Techniquesmentioning

confidence: 99%

REVIEW: Assisted reproduction in the azoospermic couple

Nicopoullos

Ramsay

Almeida

et al. 2004

BJOG

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“…Testicular biopsies were obtained from men with obstructive azoospermia (n ϭ 25) attending the Regional Fertility Centre, Belfast as previously reported (Steele et al, 2000). Patients with obstructive azoospermia were classified as men with two azoospermic semen analyses, normal FSH and LH levels and a normal karyotype to exclude men with non-obstructive azoospermia.…”

Section: Subjectsmentioning

confidence: 99%

“…Testicular biopsy and sperm preparation (Steele et al, 2000) Briefly the spermatic cord was located and 10 ml of 0.5% bupivacaine injected around it. Five minutes later the testis was firmly palpated to ensure numbness.…”

Section: Preparation Of Ejaculated Sperm By Percoll Density Centrifugmentioning

confidence: 99%

A comparison of mitochondrial and nuclear DNA status in testicular sperm from fertile men and those with obstructive azoospermia

O’Connell

McClure²,

Lewis

2002

Human Reproduction

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BACKGROUND: Mitochondria are vital to sperm as their motility powerhouses. They are also the only animal organelles with their own unique genome; encoding subunits for the complexes required for the electron transfer chain. METHODS: A modified long PCR technique was used to study mitochondrial DNA (mtDNA) in ejaculated and testicular sperm samples from fertile men undergoing vasectomy (n ⍧ 11) and testicular sperm from men with obstructive azoospermia (n ⍧ 25). Nuclear DNA (nDNA) fragmentation was measured by an alkaline gel electrophoresis (comet) assay. RESULTS: Wild-type mtDNA was detected in only 60% of fertile men's testicular sperm, 50% of their ejaculated sperm and 46% of testicular sperm from men with obstructive azoospermia. The incidence of mitochondrial deletions in testicular sperm of fertile and infertile men was not significantly different, but the mean size of the deletions was significantly less in testicular sperm from fertile men compared with men with obstructive azoospermia (P < 0.02). NDNA fragmentation in testicular sperm from fertile men and men with obstructive azoospermia was not significantly different. CONCLUSION: Multiple mtDNA deletions are common in testicular and ejaculated sperm from both fertile and infertile men. However, in males with obstructive azoospermia, the mtDNA deletions in testicular sperm are of a larger scale.Key words: human sperm/male infertility/mitochondrial and nuclear DNA/obstructive azoospermia/ROS

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Testicular sperm extraction by Trucut needle and milking of seminiferous tubules: a technique with high yield and patient acceptability

Cited by 18 publications

References 4 publications

An algorithm to predict pregnancy in assisted reproduction

An algorithm to predict pregnancy in assisted reproduction

REVIEW: Assisted reproduction in the azoospermic couple

A comparison of mitochondrial and nuclear DNA status in testicular sperm from fertile men and those with obstructive azoospermia

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