Testicular dysfunction and antioxidative defense system of Swiss mice after chromic acid exposure

Acharya, Usha; Mishra, Monalisa; Tripathy, Rashmi R.; Mishra, Ishri

doi:10.1016/j.reprotox.2005.11.004

Cited by 67 publications

(41 citation statements)

References 36 publications

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“…In vivo model studies are necessary for studying the effects of oxidative stress on testis function and sperm quality. A number of chemical components have been identified to induce oxidative stress in animal models ( [53,3,46,46,15]). TBHP is a well-known ROS inducer which has been used for induction of oxidative stress and damages in male reproductive system and spermatogenesis ( [27,26,1]).…”

Section: Discussionmentioning

confidence: 99%

TBHP-induced oxidative stress alters microRNAs expression in mouse testis

Fatemi

Sanati

Shamsara

et al. 2014

J Assist Reprod Genet

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Purpose Reactive oxygen species (ROS) and oxidative stress is one of the main reasons of male infertility. MicroRNAs (miRNAs) regulate multiple intracellular processes. Alterations in miRNAs expression may occur in different conditions and diseases. In this study, the effect of oxidative stress induced by tertiary-butyl hydroperoxide (TBHP) on the expression of candidate miRNAs in mouse testis was investigated.Methods After determining median lethal dose (LD 50 ), TBHP was intraperitoneally (ip) injected at the dilution of 1:10 LD 50 into the adult male mice for 2weeks, and then testis tissues were removed in order to assay the ROS level. Total RNA was extracted and the expression of five miRNAs was quantified by reverse transcription-real time polymerase chain reaction (RT-qPCR).Results The flow cytometry analysis showed a significant increase in ROS level in testis. The expression of three out of five selected miRNAs, including miR-34a, miR-181b and miR-122a, showed some degrees of changes following exposure to oxidative stress. These miRNAs are involved in antioxidant responses, inflammation pathway and spermatogenesis arrest. Conclusions In conclusion, TBHP alters the miRNA expression profile of testis which might play a potential role in oxidative and antioxidative responses and spermatogenesis.

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Section: Discussionmentioning

confidence: 99%

TBHP-induced oxidative stress alters microRNAs expression in mouse testis

Fatemi

Sanati

Shamsara

et al. 2014

J Assist Reprod Genet

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“…Recent studies in a few mammal species like mice (Pereira et al 2005;Acharya et al 2006), monkey (Aruldhas et al 2005;Subramanian et al 2006) and human (Li et al 2001;Danadevi et al 2003) determined that chromium acts as a reproductive toxicant. The focus of the present study was therefore, to investigate the Cr (VI) induced ovarian histopathology of a teleost fish, Channa punctatus both on acute and chronic exposures during the preparatory phase of the reproductive cycle.…”

mentioning

confidence: 99%

Histopathological Effects of Hexavalent Chromium in the Ovary of a Fresh Water Fish, Channa punctatus (Bloch)

Mishra

Mohanty

2008

Bull Environ Contam Toxicol

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The histopathological effects of hexavalent chromium (Cr VI) in the ovary of a fresh water teleost, Channa punctatus were investigated. An exposure-dependent alteration in ovarian histology is reported. For both acute and chronic exposures to Cr (VI), the percentages of atretic oocytes were increased; this increase was more pronounced in the acute exposure group. A decrease in percentage of vitellogenic oocytes was observed in the chronic exposure group indicating impairment of vitellogenesis. The hepatocellular vacuolization and atrophy along with pyknotic nuclei in both acute and chronic chromium exposed fish liver supports the vitellogenic impairment. The observed alterations may be due to both direct cytotoxic effect of Cr (VI) on the ovary as well as mediation by overall systemic toxicity affecting other vital organs.

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“…Chromium, for example, is a testicular toxicant that stimulates lipid peroxidation and suppresses antioxidant enzyme activities as well as ascorbate levels in the testes. 113 Additional studies in monkeys have also shown that chromium administration decreases not only inhibit the classical array of antioxidant enzymes in the testes but also diminishes the testicular content of GSH as well as vitamins A,E and C, while H 2 O 2 production and hydroxyl radical formation are increased. 34 Additional transition metals such as iron also induce lipid peroxidation, protein carbonyl expression and lipid soluble antioxidant depletion in testicular tissue with the consequent disruption of spermatogenesis.…”

Section: Disruption Of the Antioxidant Status Of The Testesmentioning

confidence: 99%