Testicular denervation-induced nuclear changes in Leydig cell of hemicastrated adult rats

Bao-chang, Zhu; Cavicchia, Juan C.; Chiocchio, Sara R.

doi:10.1016/s0040-8166(98)80027-x

Cited by 9 publications

(5 citation statements)

References 32 publications

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“…Anatomical, pharmacological and physiological data (Hib, 1976;Laitinen and Talo, 1981;Pholpramool and Triphon, 1984;Mirabella et al, 2006Mirabella et al, , 2008Squillacioti et al, 2008Squillacioti et al, , 2009 suggest that the role of adrenergic and cholinergic inner- (Farrel and Lyman, 1937;Sjostrand, 1965;Mirabella et al, 2007) and blood flow (Baumgarten and Holstein, 1968;Kuwahara and Frick, 1974;Damber et al, 1982;Billups et al, 1990;Santamaria et al, 1995). Epithelial exo/endocytic events and ionic exchange between cellular and luminal compartments are other processes that are influenced by autonomic innervations (Mayerhofer et al, 1992;Chan et al, 1994;Kempinas et al, 1995;Lamano-Carvalho et al, 1996;Zhu et al, 1998). Immunohistochemical studies on the mammalian epididymis generally reveal the presence of noradrenergic, cholinergic and peptidergic nerves which have a different distribution within the epididymis (Larsson, 1977;Alm et al, 1978Alm et al, , 1980Greenberg et al, 1985;LamanoCarvalho, 1986;Schindermeiser et al, 1989;Tainio, 1994;Lakomy, et al, 1997;Gürtler, 2001).…”

Section: Innervation and Immunohistochemical Characteristics Of Epidimentioning

confidence: 99%

Innervation and Immunohistochemical Characteristics of Epididymis in Alpaca Camelid (Vicugna Pacos)

Liguori

Paino

Squillacioti

et al. 2013

Italian Journal of Animal Science

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Alpacas (Vicugna pacos) are domesticated camelids indigenous to south America and recently also bred in Europe and Italy for their high quality wool. There is little data available regarding the innervation of the male reproductive tract of this species. In the present study, the distribution of protein gene product 9.5 (PGP 9.5), neuropeptide Y (NPY), tyrosine hydroxilase (TH), calcitonin gene related peptide (CGRP) and substance P (SP) was analyzed in the epididymis by using immunohistochemical methods. Specimens of the caput, corpus and cauda epididymis were fixed in Bouin's fluid and processed for immunohistochemistry analysis with primary antibodies against PGP 9.5, NPY, TH, CGRP and SP. Immunopositivity to PGP 9.5 and TH and NPY was observed in nerve fibre bundles and in single nerve fibres contained into the peritubular connective tissue. Many TH and NPY immunopositive cells were found to innervate blood vessels. Rare CGRP and SP immunopositive nerves were observed. Several PGP 9,5 and NPY immunopositive epithelial cells were observed in the caput epididymis. The results of the present study suggest a role for the innervations in modulate reproductive functions in the alpaca epididymis.

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Section: Innervation and Immunohistochemical Characteristics Of Epidimentioning

confidence: 99%

Innervation and Immunohistochemical Characteristics of Epididymis in Alpaca Camelid (Vicugna Pacos)

Liguori

Paino

Squillacioti

et al. 2013

Italian Journal of Animal Science

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“…Our previous studies in adult rats also indicate that, although the testis innervation comes from both the SSN and ISN, the latter contributes markedly (Zhu et al ., 1995), and ISN is necessary for the compensatory rise of testosterone induced by hemicastration (Zhu et al ., 2000) through Leydig cells (Zhu et al ., 1998).…”

Section: Introductionmentioning

confidence: 99%

Administration of acetylcholine to the spermatic nerve plexus inhibits testosterone secretion in an in vitro isolated rat testis–nerve plexus system

et al. 2002

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Strong evidence indicated that spermatic nerves are involved in the regulation of testosterone secretion. Our previous work showed that the inferior spermatic nerves play a more significant role than the superior ones in the regulation of testosterone secretion. However, it is unknown whether traditional neurotransmitters are involved in this regulation. In order to evaluate this point, the present experiments were carried out in an in vitro system where an isolated testis-spermatic nerve plexus preparation was incubated in two separate containers, one for the testis and the other for the nerve plexus and both interconnected by the inferior spermatic nerves. Both tissues were maintained in the same environmental conditions except for the neurotransmitter treatment, applied only to the nerve plexus. Acetylcholine can significantly inhibit the secretion of testosterone until the end of incubation. The present experiments suggest that the secretion of testosterone could be regulated, at least in part, by acetylcholine through the inferior spermatic nerves.

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“…Furthermore, various neurotransmitters display a variety of trophic effects, when available during developmental stages, in various organs including the genital tract (Buznikov et al, 1999). Ac- cordingly, published evidence suggests that autonomic innervation might be required for cell differentiation, development, and structural integrity of various male reproductive tract organs (Hodson, 1965;Nagai et al, 1982;Gerendai et al, 1984Gerendai et al, , 1989Bergh et al, 1987;Zhu et al, 1998;Gutiérrez-Ospina et al, 1999;Chow et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…Such anatomical relationships, together with pharmacological and physiological data (Hib, 1976;Laitinen and Talo, 1981;Pholpramool and Triphrom, 1984), suggest that the major role of the adrenergic and cholinergic innervation in adult animals is to control excurrent duct system contraction, sperm transport through it, and blood flow (Baumgarten et al, 1968;Kuwahara and Frick, 1974;Damber et al, 1982;Billups et al, 1990;Santamaria et al, 1995). Epithelial exo/endocytotic events and ionic exchange between cellular and luminal compartments are other processes that are influenced by autonomic innervation in adult animals (Mayerhofer et al, 1992;Chan et al, 1994;Kempinas et al, 1995;Lamano-Carvalho et al, 1996;Zhu et al, 1998).…”

mentioning

confidence: 99%

“…Pharmacological and surgical denervation experiments have also documented the importance of autonomic, and especially adrenergic, innervation for sustaining both the normal growth pattern and the rate of development of testes and other male reproductive organs (Nagai et al, 1982;Gerendai et al, 1984Gerendai et al, , 1989Bergh et al, 1987;Zhu et al, 1998;Chow et al, 2000). In fact, autonomic innervation is necessary to maintain mature ovary (Burden and Lawrence, 1977;Gerendai et al, 1978;Burden et al, 1981Burden et al, , 1983 and testicular functional and structural integrity (Hodson, 1965;Nagai et al, 1982;Bergh et al, 1987;Lamano-Carvalho et al, 1996;Zhu et al, 1998;Chow et al, 2000). It has also been suggested that autonomic innervation is even required for the process of gonadal sex determination and/or differentiation in some vertebrate species (Gutiérrez-Ospina et al, 1999).…”

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confidence: 99%

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Segment‐Specific Decrease of Both Catecholamine Concentration and Acetylcholinesterase Activity Are Accompanied by Nerve Refinement in the Rat Cauda Epididymis During Sexual Maturation

SILVA,

QUEIRÓZ,

NETO

et al. 2002

Journal of Andrology

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In the present work, histochemical and biochemical studies were conducted to analyze changes in the pattern of autonomic innervation during sexual maturation, using the rat epididymis as a model. Glyoxylic acid histochemistry and immunohistochemical studies against dopamine β‐hydroxylase (DβH) and acetylcholinesterase (AChE) indicated a reduction in the amount of catecholaminergic and AChE‐positive neurons, fibers, and puncta detected in the cauda epididymis of adult rats (120 days old), when compared to immature (40 days) and young adult (60 days) animals. No obvious age‐related variations were detected in the few catecholaminergic and AChE‐positive fibers and puncta present in the caput region. AChE‐positive fibers were found sorting out among epithelial cells and ending free upon the epithelial surface or into the tubular lumen of the cauda region of adult rats. Furthermore, a positive staining for AChE in epithelial cells was also detected in the caput and cauda epididymis in all ages studied. Biochemical analysis confirmed a significant decrease in noradrenaline concentration as well as AChE activity in the cauda epididymis with sexual maturation. Immunohistochemical studies against microtubule‐associated protein 1B (MAP 1B), a neuronal cytoskeletal marker, further substantiated the quantitative changes observed in catecholaminergic and AChE‐positive neuronal elements in the cauda epididymis. Thus, our results documented segment‐specific variations in noradrenaline concentration and AChE activity during epididymal sexual maturation and suggest that such variations result, at least in part, from the refinement of the autonomic innervation pattern with age.

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Testicular denervation-induced nuclear changes in Leydig cell of hemicastrated adult rats

Cited by 9 publications

References 32 publications

Innervation and Immunohistochemical Characteristics of Epididymis in Alpaca Camelid (Vicugna Pacos)

Innervation and Immunohistochemical Characteristics of Epididymis in Alpaca Camelid (Vicugna Pacos)

Administration of acetylcholine to the spermatic nerve plexus inhibits testosterone secretion in an in vitro isolated rat testis–nerve plexus system

Segment‐Specific Decrease of Both Catecholamine Concentration and Acetylcholinesterase Activity Are Accompanied by Nerve Refinement in the Rat Cauda Epididymis During Sexual Maturation

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