Testicular collections as a technique to increase milt availability in sauger (sander canadensis)

Blawut, Bryan; Wolfe, Barbara A.; Moraes, Christa R.; Sweet, Douglas J.; Ludsin, Stuart A.; Silva, Marco A. Coutinho da

doi:10.1016/j.anireprosci.2019.106240

Cited by 10 publications

(3 citation statements)

References 24 publications

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“…In contrast, it was reported a similar total motility of the sperm collected by stripping and abdominal massage in zebrafish compared to the dissection 29 or even higher total motility and progressive motility of the sperm collected by testes dissection in sauger ( Sander canadensis ) 30 and medaka 13 . However, in the mentioned study in sauger, a higher number of sperm with abnormal morphology was reported in testicular sperm samples 30 . An additional factor contributing to the lower sperm motility parameters in testicular sperm recorded in the present study, alongside those mentioned earlier, could be attributed to our study’s methodology wherein testicular sperm was obtained from the same fish that underwent milt collection through stripping at the short time prior to testes dissection.…”

Section: Discussionmentioning

confidence: 92%

“…These results are similar to the findings from previous studies using smelt (Osmerus eperlanus L.) 27 and Eurasian perch (Perca fluviatilis) 28 . In contrast, it was reported a similar total motility of the sperm collected by stripping and abdominal massage in zebrafish compared to the dissection 29 or even higher total motility and progressive motility of the sperm collected by testes dissection in sauger (Sander canadensis) 30 and medaka 13 . However, in the mentioned study in sauger, a higher number of sperm with abnormal morphology was reported in testicular sperm samples 30 .…”

Section: Sperm Collection Methodsmentioning

confidence: 92%

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Sperm handling and management in the teleost model fish Japanese medaka (Oryzias latipes)

Sayyari,

Krogenæs,

Mayer

et al. 2024

Sci Rep

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Japanese medaka (Oryzias latipes) has been used as a model organism in different research fields, including reproductive physiology. Sperm motility is the most important marker for male fertility in fish and, thus, reproduction success. However, because of small volume of ejaculate and short motility duration, it is still challenging to manage the sperm collection and analysis in small model fish. In the present study, we aimed to investigate sperm motility and to optimize sperm collection, short-term sperm storage, and cryopreservation in Japanese medaka (Oryzias latipes). Using two different approaches for sperm collection: testes dissection and abdominal massage, different housing conditions and activating the sperm with different activation solutions, we investigated immediate sperm motility. In the second part of this study, we used different osmolalities of immobilization solution, Hank's Balanced Salt Solution (HBSS) for sperm storage at 0, 2 and 3 h after sperm collection. Finally, the sperm were cryopreserved using methanol as cryoprotectant and HBSS as extender at two different osmolalities, and post-thaw sperm motility was investigated. The highest post-activating sperm motility was achieved in the groups activated by the extender at 300 mOsm/kg. The quality of sperm remained unaffected by co-housing with females or with males only. Furthermore, Hanks’ Balanced Salt Solution (HBSS) with an osmolality of 600 mOsm/kg demonstrated its efficacy as a suitable extender for sperm storage, preserving motility and progressivity for 3 h. The highest post-thaw motility was around 35%. There were no significant differences between post-thaw motility in different groups. We also found that post-thaw incubation on ice can maintain the motility of the sperm for up to one hour after thawing.

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Section: Discussionmentioning

confidence: 92%

Section: Sperm Collection Methodsmentioning

confidence: 92%

Sperm handling and management in the teleost model fish Japanese medaka (Oryzias latipes)

Sayyari,

Krogenæs,

Mayer

et al. 2024

Sci Rep

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“…As saugeye production is highly variable due to limited sperm production per individual sauger compounded by fluctuations in populations and weather conditions, assisted production strategies are necessary for sustaining the multimillion dollar recreational fishery industry in Ohio reservoirs. Previous research has focused on managing sperm use (Blawut et al., 2018), long‐term storage of excess spermatozoa using cryopreservation (Blawut et al., 2020) and utilizing testicular harvest to enhance sperm yield (Blawut, Wolfe, Moraes, Sweet, et al., 2020). However, sperm quality (i.e., morphology, maturation and motility) can vary as a result of the unintended consequences of these assisted reproduction techniques and thus could adversely impact saugeye production.…”

Section: Introductionmentioning

confidence: 99%

Spatial and Temporal Changes in Testis Morphology and Sperm Ultrastructure of the Sportfish Sauger (Sander canadensis)

et al. 2021

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The objective of this study was to assess testicular morphology and spermatozoal structure spatially within the reproductive tract and temporally among seasons in the sauger (Sander canadensis). The testis exists as two separate lobes joined at the urogenital pore and were characterised as unrestricted lobular with seminiferous tubules terminating at the ventral periphery and coalescing dorsally on the main sperm duct. Differences were observed between the pre‐breeding season (November) and breeding season (March), with every stage of spermatogenesis occurring in spermatocysts in pre‐breeding season in contrast to only spermatozoa being present in the tubules and main duct during the breeding season. Longitudinal folds in the main duct epithelium increased in number with increasing proximity to the urogenital pore, greatly increasing epithelial height regardless of season. Sauger spermatozoa consisted of an ovoid head, a midpiece containing 2 – 4 mitochondria incorporated into the head and a single flagellum containing an asymmetrical lateral ribbon. Motile spermatozoa were found throughout the testis during the breeding season. A decrease in sperm concentration was quantified moving proximally, suggesting a hydration effect by the main duct epithelium during the breeding season. These observations fill an important knowledge gap regarding reproductive biology of this impactful recreational fish species.

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Gonadal steroid fluctuations and reproduction results of European grayling (Thymallus thymallus L.) from broodstock farmed in a recirculation aquaculture system

Szmyt

Piech

Panasiewicz

et al. 2021

Animal Reproduction Science

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Testicular collections as a technique to increase milt availability in sauger (sander canadensis)

Cited by 10 publications

References 24 publications

Sperm handling and management in the teleost model fish Japanese medaka (Oryzias latipes)

Sperm handling and management in the teleost model fish Japanese medaka (Oryzias latipes)

Spatial and Temporal Changes in Testis Morphology and Sperm Ultrastructure of the Sportfish Sauger (Sander canadensis)

Gonadal steroid fluctuations and reproduction results of European grayling (Thymallus thymallus L.) from broodstock farmed in a recirculation aquaculture system

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