Testicular chromatin activation in hypophysectomized rats

Chiu, Jen‐Fu; Thomson, Judy; Hnilica, Lubomir S.

doi:10.1016/0005-2787(76)90185-4

Cited by 4 publications

(1 citation statement)

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“…Several reports have been published on changes in R N A synthesis, RNA content and RNA polymerase activity in the testis, measured in vivo or in vitro after in vivo stimulation with follitropin (Means 1971;Abney et al 1974;Reddy & Villee 1975) or testosterone propionate (Chiu et al 1976). Moreover, Parvinen & Siiderstrom (1976) have reported that the incorporation of 3H-uridine in testicular tubules, isolated from adult rats 3 days after hypophysectomy, was increased when the tubules were incubated in the presence of follitropin and testosterone.…”

Section: Discussionmentioning

confidence: 99%

Effect of Hypophysectomy on Ribonucleic Acid Synthesis in Primary Spermatocytes Isolated from Rat Testes

et al. 1979

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RNA synthesis was examined in pachytene spermatocytes isolated from intact immature rats and from hypophysectomized immature rats (2–4 days after hypophysectomy). Hypophysectomy did not result in changes in the synthesis and turnover of “heterogeneous” RNA as well as the synthesis and processing of rRNA from spermatocytes. The uptake and incorporation of 3H‐uridine in the spermatocyte population isolated from hypophysectomized rats, however, was decreased. These contradictory results were explained by radioautographic experiments which showed that 25 ± 6% of the pachytene spermatocytes isolated from hypophysectomized rats were unlabelled after incubation with 3H‐uridine, whereas only 3 ± 2% of the pachytene spermatocytes from intact rats were unlabelled. It appeared therefore that in the absence of gonadotropic hormones from the pituitary gland, RNA synthesis in many spermatocytes remained unchanged for at least several days, although an increased number of the spermatocytes became inactive.

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Section: Discussionmentioning

confidence: 99%