Testicular cell transplantation into the human testes

Faes, Katrien; Tournaye, Herman; Goethals, Laurence; Lahoutte, Tony; Hoorens, Anne; Goossens, Ellen

doi:10.1016/j.fertnstert.2013.06.016

Cited by 31 publications

(24 citation statements)

References 28 publications

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“…Unfortunately, this SSC transplantation procedure is technically demanding in species with several efferent ducts because, in these species they tend to be short, thin and highly convoluted ( Figure 3). This anatomical condition corresponds to most farm animals [88] , primates [89] and humans [90] . Xenogeneic transplantation of SSC cell suspensions into the mouse testis has been attempted by several groups [91] .…”

mentioning

confidence: 92%

Spermatogonial stem cells: Current biotechnological advances in reproduction and regenerative medicine

Aponte

2015

WJSC

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mentioning

confidence: 92%

Spermatogonial stem cells: Current biotechnological advances in reproduction and regenerative medicine

Aponte

2015

WJSC

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“…In the treatment of male infertility, spermatogonial stem cell transplantation (SSCT) was first reported by Brinster in 1994 [3] and has since been established as a technological breakthrough in stem cell research and the study of Sertoli cell-germ cell interactions. Autologous, homologous, and exogenous SSCT has been carried out in various species including rodents, bovines, monkeys and even humans [3,4,5,6]. Since spermatogonial stem cells (SSCs) transmit genetic information to their offspring, homologous and exogenous SSCT will inevitably be challenged in terms of reproductive ethics [7].…”

Section: Introductionmentioning

confidence: 99%

Potential Spermatogenesis Recovery with Bone Marrow Mesenchymal Stem Cells in an Azoospermic Rat Model

Zhang

Liu

Peng

et al. 2014

IJMS

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Non-obstructive azoospermia is the most challenging type of male infertility. Stem cell based therapy provides the potential to enhance the recovery of spermatogenesis following cancer therapy. Bone marrow-derived mesenchymal stem cells (BMSCs) possess the potential to differentiate or trans-differentiate into multi-lineage cells, secrete paracrine factors to recruit the resident stem cells to participate in tissue regeneration, or fuse with the local cells in the affected region. In this study, we tested whether spermatogenically-induced BMSCs can restore spermatogenesis after administration of an anticancer drug. Allogeneic BMSCs were co-cultured in conditioned media derived from cultured testicular Sertoli cells in vitro, and then induced stem cells were transplanted into the seminiferous tubules of a busulfan-induced azoospermatic rat model for 8 weeks. The in vitro induced BMSCs exhibited specific spermatogonic gene and protein markers, and after implantation the donor cells survived and located at the basement membranes of the recipient seminiferous tubules, in accordance with what are considered the unique biological characteristics of spermatogenic stem cells. Molecular markers of spermatogonial stem cells and spermatogonia (Vasa, Stella, SMAD1, Dazl, GCNF, HSP90α, integrinβ1, and c-kit) were expressed in the recipient testis tissue. No tumor mass, immune response, or inflammatory reaction developed. In conclusion, BMSCs might provide the potential to trans-differentiate into spermatogenic-like-cells, enhancing endogenous fertility recovery. The present study indicates that BMSCs might offer alternative treatment for the patients with azoospermatic infertility after cancer chemotherapy.

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“…The technique of SSC transplantation introduced by Brinster and Avarbock (1994) has been developed in detail on rodent models. The conditions for injecting SSCs into human testes have been optimised (Faes et al 2013). Recently differentiation of human and murine pluripotent stem cells (PSC) to germ cells has been demonstrated (Nakaki et al 2013;Durruthy Durruthy et al 2014) providing a source of germ cells for use in further experimentation and potential clinical applications.…”

Section: Discussionmentioning

confidence: 99%

Neonatal testicular cell transplantation restores murine spermatogenesis damaged in the course of herpes simplex virus-induced orchitis

Малолина

Kulibin

Кущ

2016

Reprod. Fertil. Dev.

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Genital tract infection and inflammation may affect male fertility, causing germ and Sertoli cell loss. We determined if testicular cell transplantation is effective at repairing testicular injury induced by herpes simplex virus (HSV) orchitis. ROSA26 mice were used as donors and the recipients were C57BL/6 mice after HSV testicular inoculation; some of the recipients were treated with the antiviral drug acyclovir (ACV). ACV reduced the amount of HSV antigen in testes on Day 3 after transplantation and enhanced the efficacy of transplantation at Day 30. In recipient testes, donor Sertoli cells formed new seminiferous tubules; significantly more new tubules were observed in the testes of ACV-treated mice compared with mice not treated with ACV (17.8% vs 3.6%). Over half (50.4%) of new tubules in ACV-treated testes contained germ cells and round spermatids were detected in 14.2% of new tubules compared with 15.9% and 5.3% in testes not treated with ACV, respectively. At Day 150 the seminiferous epithelium was completely recovered in some donor tubules and elongated spermatids were observed inside it. Thus, our findings reveal the effectiveness of the combination of antiviral therapy with neonatal testis-cell transplantation for the restoration of spermatogenesis damaged by viral infection.

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Testicular cell transplantation into the human testes

Cited by 31 publications

References 28 publications

Spermatogonial stem cells: Current biotechnological advances in reproduction and regenerative medicine

Spermatogonial stem cells: Current biotechnological advances in reproduction and regenerative medicine

Potential Spermatogenesis Recovery with Bone Marrow Mesenchymal Stem Cells in an Azoospermic Rat Model

Neonatal testicular cell transplantation restores murine spermatogenesis damaged in the course of herpes simplex virus-induced orchitis

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