Testicular biometry, ultrastructure, cellular cytometry and hormonal profile in relation to age in Egyptian buffaloes.

Abstract

This study was conducted on a number of (30) buffaloes in Qalubia. The age ranged from 12 to 36 month. There were divided into two groups: group (I) and group (II), their ages ranged from <18 month and ≥18 till 36 months, respectively. Testosterone levels in the blood, testicular biometry by the optical and the electronic microscope, and finally flow cytometry of the testicular cells were estimated. it was concluded that: The testosterone hormone level in the blood in group (I) and in group (II) was 1.9 ± 0.48 and 1.07 ± 0.35 ng/ ml serum prospectively, weight, length and width of the testicle in group (I) was 75± 13.8 gm., 7.3 ± 0.48cm and 4.22 ± 0.31 cm, respectively, and in group (II) was 118.38 ± 11.29 gm., 8.12 ± o.63 cm, and 5.0 ± 0.23 cm, respectively. The diameter of the seminiferous tubules in group (I) was 99.90 ± 17.4µm, while in group (II) was 104.6 ± 15.82 µm. The number of Leydig cells in group (I) was 40.14 ± 15.52, and in group (II) was 49.16 ± 27.99. The lining epithelium thickness of the seminiferous tubules in group (I) was 26.06 ± 5.51 µm, and in group (II) was 27.64 ±5.52 µm, while degree of cell degeneration in group (I) was 6.12±0.79 and in group (II) was 4.62±0.66. By using flow cytometer there was difference between 2 age group in the testicular cell and apoptosis. An electron micrograph showing difference in the testicular cells' ultrastructure Buffalo bull Electron microscope.