Testicular Androgen Binding Protein (ABP) — A Parameter of Sertoli Cell Secretory Function

Hansson, V.; Weddington, S. C.; Næss, Oddvar; Attramadal, Arne; French, Frank S.; Kotite, Nicolas J.; Nayfeh, Shihadeh N.; Ritzén, E. M.; Hagenäs, Lars

doi:10.1007/978-1-4613-4440-7_23

Cited by 33 publications

(16 citation statements)

References 14 publications

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“…One of the major proteins regulated by FSH and secreted by Sertoli cells into the lumen is ABP [22,26]. In the present study, LM immunocytochemistry revealed that ABP was expressed intensely in Sertoli cells, but the reaction was dramatically reduced in FORKO mice, a finding confirmed by the Western blot analyses.…”

Section: Discussionsupporting

confidence: 84%

“…Regulation of ABP by FSH and testosterone has been demonstrated [25], although whether one or both are required for complete function remains to be resolved [22]. ABP also displays a stage-specific expression pattern within the seminiferous epithelium and its secretion has often been regarded as an index of Sertoli cell function [22,26]. Recent work with transgenic mice overexpressing rat ABP has shed more light on the role of ABP in spermatogenesis.…”

Section: Introductionmentioning

confidence: 99%

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Structural and Functional Modifications of Sertoli Cells in the Testis of Adult Follicle-Stimulating Hormone Receptor Knockout Mice1

Grover

Sairam

Smith

et al. 2004

Biology of Reproduction

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Follicle stimulating hormone (FSH) plays important roles during testicular development and in the maintenance of spermatogenesis in the adult. However, the cellular events or pathways that FSH regulates to achieve these effects in Sertoli cells, where the FSH receptors (FSH-R) are located, is still not fully elucidated. The development of FSH-R knockout (FORKO) mice provides a model to examine alterations in testicular structure and function in its absence. To this end, light (LM) and electron microscopic (EM) analyses of perfusion-fixed testes of wild-type and FORKO mice of different ages were performed. Under the LM, a significant reduction was noted in the profile area of seminiferous tubules of FORKO mice compared with their wild-type counterparts at different ages. In addition, FORKO testes revealed large irregularly shaped spaces within the seminiferous epithelium, extending from the base to the lumen. Such spaces were often separated by anastomotic cords of spherical germ cells or completely surrounded elongating spermatids. This phenotype was restricted to half or less of the circumference of only some tubules, but was seen at all stages. EM analyses revealed that the spaces corresponded to an apparent accumulation of fluid in the Sertoli cell cytoplasm, coincident with an absence of the fine flocculent ground substance seen in wild-type mice. However, the Sertoli organelles, while less prominent, appeared intact and to be floating in the enlarged fluid-filled cytoplasm. Functionally, androgen-binding protein (ABP), a major secretory protein of Sertoli cells, was dramatically reduced in FORKO mice. These results suggest that FSH-R signaling normally maintains water balance in Sertoli cells in addition to regulating ABP production.

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Section: Discussionsupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Structural and Functional Modifications of Sertoli Cells in the Testis of Adult Follicle-Stimulating Hormone Receptor Knockout Mice1

Grover

Sairam

Smith

et al. 2004

Biology of Reproduction

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“…HORMONAL CONTROL OF ABP CONCENTRATIONS Chronic FSH treatment of immature or long-term hypophysectomized adult rats in creased testicular and epididymal ABP levels, suggesting that both synthe sis and transport to the epididymis were stimulated (21,46). These effects of FSH are similar in hypophysectomized or immature hormone-deficient animals.…”

Section: Synthesis and Metabolism Of Abpmentioning

confidence: 74%

Extracellular Androgen Binding Proteins

Bardin¹,

Musto²,

Gunsalus³

et al. 1981

Annu. Rev. Physiol.

113

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“…It has also been proposed as a biochemical marker for Sertoli cell function and formation of the blood-testis barrier (43). Furthermore, ABP bound to androgen has been found to contribute to the regulation of fertility and spermatogenesis in various experimental models (44,45).…”

Section: Discussionmentioning

confidence: 99%

Infertility with defective spermatogenesis and hypotestosteronemia in male mice lacking the androgen receptor in Sertoli cells

Chang

Chen

Yeh

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

413

293

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Androgens and the androgen receptor (AR) play important roles in male fertility, although the detailed mechanisms, particularly how androgen͞AR influences spermatogenesis in particular cell types, remain unclear. Using a Cre-Lox conditional knockout strategy, we generated a tissue-specific knockout mouse with the AR gene deleted only in Sertoli cells (S-AR ؊/y ). Phenotype analyses show the S-AR ؊/y mice were indistinguishable from WT AR mice (B6 AR ؉/y ) with the exception of testes, which were significantly atrophied. S-AR ؊/y mice were infertile, with spermatogenic arrest predominately at the diplotene premeiotic stage and almost no sperm detected in the epididymides. S-AR ؊/y mice also have lower serum testosterone concentrations and higher serum leuteinizing hormone concentrations than B6 AR ؉/y mice. Further mechanistic studies demonstrated that S-AR ؊/y mice have defects in the expression of anti-Mü llerian hormone, androgen-binding protein, cyclin A1, and sperm-1, which play important roles in the control of spermatogenesis and͞or steroidogenesis. Together, our Sertoli cell-specific AR knockout mice provide in vivo evidence of the need for functional AR in Sertoli cells to maintain normal spermatogenesis and testosterone production, and ensure normal male fertility. knockout mice ͉ anti-Mü llerian hormone ͉ testosterone

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Testicular Androgen Binding Protein (ABP) — A Parameter of Sertoli Cell Secretory Function

Cited by 33 publications

References 14 publications

Structural and Functional Modifications of Sertoli Cells in the Testis of Adult Follicle-Stimulating Hormone Receptor Knockout Mice1

Structural and Functional Modifications of Sertoli Cells in the Testis of Adult Follicle-Stimulating Hormone Receptor Knockout Mice1

Extracellular Androgen Binding Proteins

Infertility with defective spermatogenesis and hypotestosteronemia in male mice lacking the androgen receptor in Sertoli cells

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