Testicular Abnormalities in Male Rats After Lactational Exposure to Nonylphenols

Lee, Ping C.; Arndt, Paul; Nickels, Katherine C.

doi:10.1385/endo:11:1:61

Cited by 39 publications

(25 citation statements)

References 25 publications

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“…In this paper, the level of NP in the animal body was not estimated, however the effects observed at 5 weeks might be partly dependent on this phenomenon. Several authors have observed that NP causes testicular abnormalities such as a lack of differentiation of seminiferous tubules and decreased sperm count and motility [37][38][39]. In the present study, the diminished sperm count after treatment with NP did not correlate with decreased testes weights.…”

Section: Discussioncontrasting

confidence: 53%

Combined action of X-rays and nonylphenol on mouse sperm

Dobrzyńska

2011

Open Life Sciences

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The aim of this study was to assess the effects of 2-weeks’ X-ray and/or nonylphenol (NP) exposure on male mice’s sperm count and quality. Pzh:SFIS mice were exposed to X-rays (0.05 Gy, 0.10 Gy, 0.20 Gy) or to nonylphenol (25 mg/kg bw, 50 mg/kg bw, 100 mg/kg bw) or to both agents (0.05 Gy + 25 mg/kg bw NP, 0.10 Gy + 50 mg/kg bw NP). At 24 h and 5 weeks after the end of exposure the sperm count, morphology and frequency of DNA damage in the male germ cells were estimated. Each agent alone diminished sperm count and morphology. The dose of 0.05 Gy of X-rays decreased the frequency of DNA damage. Combined exposure to lower doses of both agents significantly improved sperm morphology and decreased the level of DNA damage compared to one agent alone. Combined exposure to higher doses reduced the frequency of DNA damage compared to the effect of the appropriate dose of NP. Results of combined exposure to low doses of both agents suggest that 0.05 Gy of X-rays stimulate the DNA damagecontrol system and in consequence repair of DNA caused by X-rays and NP. It may be correlated with increased antioxidant capacity.

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Section: Discussioncontrasting

confidence: 53%

Combined action of X-rays and nonylphenol on mouse sperm

Dobrzyńska

2011

Open Life Sciences

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“…NP could induce oxidative stress, cytotoxicity and apoptosis through endoplasmic reticulum stress in testicular sertoli cell in rat. Exposure of NP to neonatal rat has adverse effects with various reproductive parameters including decrease in the size of testis, reduced sperm count and sperm motility (Lee et al, 1999). In our experiments, NP 30 μM significantly decreased percentages of most boar sperm characteristics such as motility, viability, membrane integrity and mitochondrial activity for each 3, 6 or 9 hr incubation period (p<0.05).…”

Section: Discussionsupporting

confidence: 46%

“…In our experiments, NP 30 μM significantly decreased percentages of most boar sperm characteristics such as motility, viability, membrane integrity and mitochondrial activity for each 3, 6 or 9 hr incubation period (p<0.05). These results indicate that NP as endocrine disruptor also has direct detrimental effect on sperm characteristics despite of indirect effect mediated through the testicular tissue degeneration (Lee et al, 1999;El-Dakdoky and Helal, 2007). Addition of 10, 50 or 100 μM of DBP decreased overall boar sperm characteristics with dosedependency for 3, 6 or 9 hr incubation period (p<0.05).…”

Section: Discussionmentioning

confidence: 79%

Effects of Endocrine Disruptors (NP, DBP and BPA) on Sperm Characteristics and Development of IVF Embryos in Pig

Yuh

Cheong²,

Kim³

et al. 2013

Journal of Animal Science and Technology

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This study was to examine single or combined in vitro effects of environmental endocrine disruptors on boar sperm characteristics, oxidative stress damage in sperm and development of porcine IVF embryos. Addition of various concentration of NP (10, 20, 30μM), DBP (10, 50, 100 μM) and BPA (1, 5 or 10 μg/ml) on boar sperm characteristics such as percentages of sperm motility, viability, membrane integrity and mitochondrial activity were dose-dependently decreased within 3, 6 or 9 hr incubation period (p<0.05). The overall detrimental effects increased with incubation time increasement. NP, DBP and BPA showed the detrimental effects on sperm membrane and mitochondria of energy production organelles affecting cell viability with the dependancy of dose and incubation time.In combination effects, NP (10 μM) + DBP (10 μM) significantly decreased boar general sperm characteristics for 3 or 6 hr incubation period compared with control (p<0.05). When both of NP and DBP concentrations (NP; 30 μM, DBP; 100 μM) increase, the detrimental effects on sperm characteristics were larger than those of low concentration combination (p<0.05). The inhibitory effects of NP (30 μM) + BPA (10 μg/ml) on sperm characteristics were larger than those of NP (10 μM) + BPA (1 μg/ml) (p<0.05). DBP (100 μM) + BPA (10 μg/ml) decreased sperm characteristics compared with the low concentration combination (DBP 10 μM + BPA 1 μg/ml, p<0.05). This result indicates the detrimental effects of both chemicals on sperm characteristics were dose dependent. Addition of NP (30 μM) + DBP (100 μM), NP (30 μM) + BPA (10 μg/ml), DBP (10 μM) + BPA (1 μg/ml) or DBP (100 μM) + BPA (10 μg/ml) significantly increased lipid peroxidation for 3 or 6 hr incubation period (p<0.05) compared with no addition control.NP (≥ 20 μM) decreased the percentages of IVF embryo development from morulae and blastocyst stages (p<0.05) and its detrimental effects were dose-dependant. BPA 0, 1, 5 or 10 μg/ml decreased significantly and dose-dependently the percentage of morulae plus and blastocysts (p<0.05). Combinations of DBP (100 μM) plus NP (30 μM) and DBP (100 μM) plus BPA (10 μg/ml) did not affect on morulae and blastocyst development, but NP (30 μM) plus BPA (10 μg/ml) has significant detrimental effect on embryo development at these stages (p<0.05). These overall results indicate that the partial detrimental effects on boar sperm characteristics and embryo development by NP, DBP, BPA or the combination of these chemicals might be due to the increasement of lipid peroxidation and free radical formation in the cell and there were no specific interaction effects on boar sperm and embryo degeneration among the combined treatments.

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“…Exposure to nonylphenol decreases the diameter of seminiferous tubule, lumen and epithelial thickness leading to testicular atrophy and cryptorchidism (de Jager et al 1999a). Gestational, lactational or direct exposure to nonylphenol has been shown to decrease testicular and epididymal masses (de Jager et al 1999b;Lee et al 1999). Nonylphenol produced a reduction in the activity of 17a-hydroxylase/C 17,20 -lyase in testicular homogenates of neonatal, pubertal and adult rats (Laurenzana et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Effect of nonylphenol on the antioxidant system in epididymal sperm of rats

Chitra

Latchoumycandane

Mathur

2002

Archives of Toxicology

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Nonylphenol, an environmental contaminant, has been shown to induce reproductive abnormalities in male rats. The nature and mechanism of action of nonylphenol on the epididymal sperm has not been elucidated. In the present study we have sought to investigate whether administration of nonylphenol induces oxidative stress in rat epididymal sperm. Nonylphenol was administered orally to male rats at 1, 10 and 100 microg/kg body weight per day for 45 days. Twenty-four hours after the last treatment, rats were weighed and killed using anaesthetic ether. The body weight of the animals treated with nonylphenol did not show any significant change. The weights of the testes and epididymides decreased significantly whereas the weights of seminal vesicles and ventral prostate remained unchanged at all doses of nonylphenol in treated rats. Epididymal sperm were collected by cutting the epididymides into small pieces in Ham's F-12 medium at 32 degrees C. Administration of nonylphenol decreased the epididymal sperm counts in a dose-dependent manner. The activities of antioxidant enzymes superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase decreased significantly while the levels of H(2)O(2) generation and lipid peroxidation increased significantly in the animals treated with nonylphenol when expressed in terms of milligram protein and milligram DNA. The activity of alpha-glucosidase, a negative control against antioxidant enzymes, in the sperm of nonylphenol-treated rats did not show any significant change at any of the doses. The results suggest that graded doses of nonylphenol elicit depletion of antioxidant defence system in sperm, indicating nonylphenol-induced oxidative stress in the epididymal sperm of rats.

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Testicular Abnormalities in Male Rats After Lactational Exposure to Nonylphenols

Cited by 39 publications

References 25 publications

Combined action of X-rays and nonylphenol on mouse sperm

Combined action of X-rays and nonylphenol on mouse sperm

Effects of Endocrine Disruptors (NP, DBP and BPA) on Sperm Characteristics and Development of IVF Embryos in Pig

Effect of nonylphenol on the antioxidant system in epididymal sperm of rats

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