Testican in Human Blood1

BaSalamah, Mohammad A.; Marr, Henry S.; Duncan, Andrew W.; Edgell, Cora‐Jean S.

doi:10.1006/bbrc.2001.4900

Cited by 17 publications

(16 citation statements)

References 21 publications

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“…We next tested whether Cow can be secreted, reminiscent of its human homolog Testican-2 [37]. We generated an EGFP-Cow fusion construct (SP-EGFP-Cow) and a construct of Cow with a truncated signal peptide (Flag-tSP-Cow).…”

Section: Resultsmentioning

confidence: 99%

Carrier of Wingless (Cow), a Secreted Heparan Sulfate Proteoglycan, Promotes Extracellular Transport of Wingless

Chang

Sun

2014

PLoS ONE

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Morphogens are signaling molecules that regulate growth and patterning during development by forming a gradient and activating different target genes at different concentrations. The extracellular distribution of morphogens is tightly regulated, with the Drosophila morphogen Wingless (Wg) relying on Dally-like (Dlp) and transcytosis for its distribution. However, in the absence of Dlp or endocytic activity, Wg can still move across cells along the apical (Ap) surface. We identified a novel secreted heparan sulfate proteoglycan (HSPG) that binds to Wg and promotes its extracellular distribution by increasing Wg mobility, which was thus named Carrier of Wg (Cow). Cow promotes the Ap transport of Wg, independent of Dlp and endocytosis, and this function addresses a previous gap in the understanding of Wg movement. This is the first example of a diffusible HSPG acting as a carrier to promote the extracellular movement of a morphogen.

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Section: Resultsmentioning

confidence: 99%

Carrier of Wingless (Cow), a Secreted Heparan Sulfate Proteoglycan, Promotes Extracellular Transport of Wingless

Chang

Sun

2014

PLoS ONE

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“…Similarly, the proteoglycan form of testican-1 is converted to smaller stable forms in samples of human blood plasma (27). At present the physiological significance of testican cleavage is not clear, but for testican-1 it was proposed that size conversion may be important for activation or decay.…”

Section: Discussionmentioning

confidence: 99%

Mouse Testican-2

Schnepp

Lindgren

Hülsmann

et al. 2005

Journal of Biological Chemistry

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Mouse testican-2 was cloned, sequenced, and shown to be a proteoglycan with a multidomain structure closely similar to that of the human ortholog, previously described as a calcium binding extracellular matrix molecule of the BM-40/SPARC/osteonectin family (Vannahme, C., Schü bel, S., Herud, M., Gö sling, S., Hü lsmann, H., Paulsson, M., Hartmann, U., and Maurer, P. (1999). J. Neurochem. 73, 12-20). Recombinant mouse testican-2 was used to prepare specific antibodies that allowed the detection of testican-2 in various brain structures but also in lung, testis, and in several endocrine glands. Although the testican-2 expressed in EBNA-293 cells carried both heparan sulfate and chondroitin/dermatan sulfate glycosaminoglycan chains, the tissue form always contained only heparan sulfate. Both tissue-derived and recombinant testican-2 carried Nlinked glycans. Tissue-derived forms of testican-2 were detected as proteoglycans of varying size, whereas a portion of the molecules produced by EBNA-293 cells were core proteins, lacking glycosaminoglycans. Both the proteoglycan and core protein forms of testican-2 inhibited neurite extension from cultured primary cerebellar neurons and may play regulatory roles in the development of the central nervous system.

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“…In man, testican-1 is expressed by neurons and by endothelial cells of a variety of tissues (36). An unstable 130-kDa form is present in blood and converted to a smaller stable form by unidentified plasma serine proteases (37).…”

mentioning

confidence: 99%

Characterization of SMOC-1, a Novel Modular Calcium-binding Protein in Basement Membranes

Vannahme¹,

Smyth²,

Miosge³

et al. 2002

Journal of Biological Chemistry

103

113

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We have isolated the novel gene SMOC-1 that encodes a secreted modular protein containing an EF-hand calcium-binding domain homologous to that in BM-40. It further consists of two thyroglobulin-like domains, a follistatin-like domain and a novel domain. Recombinant expression in human cells showed that SMOC-1 is a glycoprotein with a calcium-dependent conformation. Results from Northern blots, reverse transcriptase-PCR, and immunoblots revealed a widespread expression in many tissues. Immunofluorescence studies with an antiserum directed against recombinant human SMOC-1 demonstrated a basement membrane localization of the protein and additionally its presence in other extracellular matrices. Immunogold electron microscopy confirmed the localization of SMOC-1 within basement membranes in kidney and skeletal muscle as well as its expression in the zona pellucida surrounding the oocyte.

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Testican in Human Blood1

Cited by 17 publications

References 21 publications

Carrier of Wingless (Cow), a Secreted Heparan Sulfate Proteoglycan, Promotes Extracellular Transport of Wingless

Carrier of Wingless (Cow), a Secreted Heparan Sulfate Proteoglycan, Promotes Extracellular Transport of Wingless

Mouse Testican-2

Characterization of SMOC-1, a Novel Modular Calcium-binding Protein in Basement Membranes

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