Testes morphology and the identification of transcripts of the hormonal pathways of the velvetbean caterpillar Anticarsia gemmatalis Hübner, 1818 (Lepidoptera: Erebidae)

Pezenti, Larissa Forim; Levy, Sheila Michele; Souza, Rui Fragassi; Sosa–Gómez, Daniel Ricardo; Rosa, Renata da

doi:10.1016/j.asd.2021.101111

Cited by 3 publications

(5 citation statements)

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“…Azadirachtin negatively affected the organization, distribution and development of germ cells in S. eridania larvae. The morphology of the testes of the southern armyworm S. eridania is similar to the morphology of the testes found for other lepidopteran species for the larval period 31–35 …”

Section: Discussionsupporting

confidence: 72%

“…The morphology of the testes of the southern armyworm S. eridania is similar to the morphology of the testes found for other lepidopteran species for the larval period. [31][32][33][34][35] Changes in the testes, such as reduction of cysts, were the most common symptoms found in azadirachtin or other plant-based insecticide expositions. 14,15,31,36 This symptom may be related to blocking or halting cell divisions in germ cells due to cell proliferation inhibition.…”

Section: Discussionmentioning

confidence: 99%

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Azadirachtin‐based insecticide impairs testis morphology and spermatogenesis of the southern armyworm Spodoptera eridania (Lepidoptera: Noctuidae)

Scudeler

Daquila

Carvalho

et al. 2023

Pest Management Science

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Background: In the search for alternative tools for integrated pest management, azadirachtin, a botanical insecticide, has been used with the most promising activity against Spodoptera spp., but the mechanism of cytotoxicity on reproductive organs remains unclear. Spodoptera eridania (Stoll, 1782) is a polyphagous pest with great economic importance that has become an important target to elucidate the action of azadirachtin on the reproductive organs of insect pests, helping to understand the deleterious effects caused by its exposure. This study evaluated the effects of chronic exposure to azadirachtin on the morphology and ultrastructure of S. eridania larval testes as well as larval development.Results: Azadirachtin exposure (6 or 18 mg a.i. L −1 ) caused a progressive increase in cumulative mortality and reduced gain in body mass after 5 days. Testicular structure indicated a reduction in their size with internal morphological changes such as spermatogonia, spermatogonial, spermatocytes and spermatid cysts in degeneration. The occurrence of cell death in germ and somatic cells was evidenced by the TUNEL technique. Electron microscopy revealed changes in cystic cells, such as cytoplasmic membrane rupture and cytoplasmic vacuolization. Chromatin compaction, changes in the rough endoplasmic reticulum and Golgi complex cisternae were observed in germ cells. Apoptotic bodies occurred between germ cell cysts.Conclusion: Azadirachtin damaged the testes of S. eridania larvae, and these changes compromised spermatogenesis and consequently the development of the reproductive potential of this specimen, making azadirachtin a promising botanical insecticide for application in integrated pest management programs.

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Azadirachtin‐based insecticide impairs testis morphology and spermatogenesis of the southern armyworm Spodoptera eridania (Lepidoptera: Noctuidae)

Scudeler

Daquila

Carvalho

et al. 2023

Pest Management Science

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“…Data were recorded daily. The sex of dead larvae was determined using the method described by Zhu 36 and Pezenti et al 37 If the larvae died, we dissected the gonads to determine larval sex. Larvae without testes were identified as females (Supporting Information, Fig.…”

Section: Survival Of Exposed Leaf Feeders and Stem Borers Under Diffe...mentioning

confidence: 99%

“…S1). [36][37][38][39][40] Also, the sex of dead pupae was determined from the caudal genitalia. The formulas for the survival rates of the four pests under different light intensities are as follows:…”

Section: Survival Of Exposed Leaf Feeders and Stem Borers Under Diffe...mentioning

confidence: 99%

Light intensity differentially mediates the life cycle of lepidopteran leaf feeders and stem borers

Xu,

Wu,

Luo

et al. 2024

Pest Management Science

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BACKGROUNDLeaf feeders, such as Spodoptera frugiperda and S. litura, and stem borers Ostrinia furnacalis and Chilo suppressalis, occupy two different niches and are well adapted to their particular environments. Borer larvae burrow and inhabit interior of stems, which are relatively dark. By contrast, the larvae of leaf feeders are exposed to sunlight during feeding. We therefore designed series of experiments to evaluate the effect of light intensity (0, 2000, and 10 000 lx) on these pests with different feeding modes.RESULTSThe development of all four pests was significantly delayed at 0 lx. Importantly, light intensity affected the development of both male and female larvae of borers but only significantly affected male larvae of leaf feeders. Furthermore, the proportion of female offspring of leaf feeders increased with increasing light intensity (S. frugiperda: 33.89%, 42.26%, 57.41%; S. litura: 38.90%, 51.75%, 65.08%), but no significant differences were found in stem borers. This research also revealed that the survival rate of female leaf feeders did not vary across light intensities, but that of males decreased with increasing light intensity (S. frugiperda: 97.78%, 85.86%, 61.21%; S. litura: 95.83%, 73.54%, 58.99%).CONCLUSIONThese results improve understanding of how light intensity affects sex differences of important lepidopteran pests occupying different feeding niches and their ecological interactions with abiotic factors in agroecosystems.This article is protected by copyright. All rights reserved.

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“…Most studies are associated with hormonal activity in the accessory glands; however, its primary function is related to stimulating the reproduction of females during copulation (Parthasarathy et al, 2009). It is known that JH mediates indirect morphogenetic relationships between different tracts and physiological systems with male sexual development in certain insect species (Contreras-Garduño et al, 2011;Flatt & Kawecki, 2007;Fry, 2006;Pezenti et al, 2021). Additionally, the ingestion of methoprene, a JH analog, promotes faster development of testes in Bactrocera tryoni Froggatt (Diptera: Tephritidae) (Adnan et al, 2020).…”

Section: Pyriproxyfen On Male Reproductionmentioning

confidence: 99%

Morphological and Morphometric Parameters of the Reproductive Organs of Euschistus heros (Hemiptera: Pentatomidae) Treated With a Sublethal Juvenile Hormone Analog

Cremonez¹,

Matsumoto²,

Perier³

et al. 2023

JAS

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The stinkbug Euschistus heros is an important pest of Brazilian agricultural production with increasing importance in the Neotropical region. High reproductive potential and increased risk of resistance to insecticides are determining factors influencing its pest status. This work evaluated the effects of pyriproxyfen on the reproduction and morphological development of the reproductive system of E. heros. Pyriproxyfen was utilized at sublethal concentrations (LC30) in fourth-instar nymphs (N4). Sublethal pyriproxyfen resulted in a reduction in fertility and fecundity of 2 and 12% when only the males were treated, 47 and 53% when only the females were treated, and 32 and 46% when females and males were treated. Basal oocytes were larger and more previtellogenic in newly emerged pyriproxyfen-treated females. The nuclear area of testicular accessory cells shrunk after 10 and 15 days of age in treated insects. Similarly, testicular shape was altered, changing from an ellipsoid to an obpyriform pattern, characterized by hypertrophy of the basal region over time. The apparent area of the organ grew until ten days of age, then shrunk at 15 days of age. In summary, pyriproxyfen treatments disrupted the normal development of N4 of E. heros and diminished the reproductive potential of female and male adults.

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Testes morphology and the identification of transcripts of the hormonal pathways of the velvetbean caterpillar Anticarsia gemmatalis Hübner, 1818 (Lepidoptera: Erebidae)

Cited by 3 publications

References 57 publications

Azadirachtin‐based insecticide impairs testis morphology and spermatogenesis of the southern armyworm Spodoptera eridania (Lepidoptera: Noctuidae)

Azadirachtin‐based insecticide impairs testis morphology and spermatogenesis of the southern armyworm Spodoptera eridania (Lepidoptera: Noctuidae)

Light intensity differentially mediates the life cycle of lepidopteran leaf feeders and stem borers

Morphological and Morphometric Parameters of the Reproductive Organs of Euschistus heros (Hemiptera: Pentatomidae) Treated With a Sublethal Juvenile Hormone Analog

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